Schlack‐Kumpf Reaction

Abstract

The synthesis of 2‐thiohydantoins from α‐amino acids with thiocyanate and acetic anhydride is simply called as the Johnson reaction. This reaction has been used to sequence peptides and proteins from the C‐terminal therefore, this reaction is known as the Schlack–Kumpf reaction. The study finds that C‐terminal amino acids and other amino acids have difficulty forming the corresponding 2‐thiohydantoin derivatives. This reaction has been used for the preparation of 2‐thiohydantoins and for the sequencing of peptide.