Schizosaccharomyces pombe as a model organism for studies of chromosome segreration

Požgajová, Miroslava; Androvic, Peter; Trakovická, Anna

doi:10.15414/afz.2015.18.02.48-52

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…Meiosis, although an event during sexual reproduction, is also induced in nitrogen deficient media, and the literature describes that it allows DNA repair, homologous recombination events, and adaptive survival advances [ 59 ]. Remarkably, several Duf999 family proteins, involved in rapidly and efficiently healing double-strands breaks near sub-telomeres and to maintain cell viability, were overexpressed in glycerol/acetate.…”

Section: Discussionmentioning

confidence: 99%

High-throughput transcriptome sequencing and comparative analysis of Escherichia coli and Schizosaccharomyces pombe in respiratory and fermentative growth

et al. 2021

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In spite of increased complexity in eukaryotes compared to prokaryotes, several basic metabolic and regulatory processes are conserved. Here we explored analogies in the eubacteria Escherichia coli and the unicellular fission yeast Schizosaccharomyces pombe transcriptomes under two carbon sources: 2% glucose; or a mix of 2% glycerol and 0.2% sodium acetate using the same growth media and growth phase. Overall, twelve RNA-seq libraries were constructed. A total of 593 and 860 genes were detected as differentially expressed for E. coli and S. pombe, respectively, with a log2 of the Fold Change ≥ 1 and False Discovery Rate ≤ 0.05. In aerobic glycolysis, most of the expressed genes were associated with cell proliferation in both organisms, including amino acid metabolism and glycolysis. In contrast in glycerol/acetate condition, genes related to flagellar assembly and membrane proteins were differentially expressed such as the general transcription factors fliA, flhD, flhC, and flagellum assembly genes were detected in E. coli, whereas in S. pombe genes for hexose transporters, integral membrane proteins, galactose metabolism, and ncRNAs related to cellular stress were overexpressed. In general, our study shows that a conserved "foraging behavior" response is observed in these eukaryotic and eubacterial organisms in gluconeogenic carbon sources.

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Section: Discussionmentioning

confidence: 99%

High-throughput transcriptome sequencing and comparative analysis of Escherichia coli and Schizosaccharomyces pombe in respiratory and fermentative growth

et al. 2021

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Design, synthesis, pharmacological evaluation and DNA interaction studies of binuclear Pt(II) complexes with pyrazolo[1,5‐a]pyrimidine scaffold

Lunagariya

Thakor

Waghela

et al. 2018

Applied Organom Chemis

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Substituted pyrazolo[1,5-a]pyrimidine ligands were synthesized by cyclization, using 3-(thiophen-2-yl)-1H-pyrazol-5-amine with substituted enones (3-phenyl-1-(pyridin-2-yl)prop-2-en-1-one) in presence of KOH and DMF as solvent to form cyclic aromatic compounds. The substituted pyrazolo[1,5-a] pyrimidine based binuclear Pt II complexes containing neutral tetradentated ligands have general formula [Pt 2 (5a-5f)Cl 4 ], (where, (5a -5f) = pyrazolo[1,5-a] pyrimidine ligand). This compounds were characterized by physicochemical and spectroscopic method like elemental analyses, UV-Visible, FT-IR, EDX, TGA, molar conductivity, magnetic susceptibility measurements, mass spectroscopy, 1 H and 13 C NMR method. The square planar geometry was predicted by electronic spectral study. All Pt II compounds were evaluated by antimicrobial assay, in vitro brine shrimp assay, in vivo cellular level bioassay using S. Pombe cells and anti-tuberculosis study. LC 50 (50% lethal concentration) values of compounds are observed between 6.450 -102.07 μg/mL. UV-vis absorption titration, competitive displacement assay, molecular docking and viscosity measurement were carried out to examine the binding type and binding strength of complexes. The binding studies suggest partial intercalative binding mode of the complexes and the observed binding constant (K b ) values are found in the order of 6d > 6b > 6c > 6a > 6e > 6 f. The anti-proliferative cytotoxicity of the synthesized Pt II complexes (6a-6f) were tested against the HCT-116 (Human Colorectal Carcinoma) cancer cell line.

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Schizosaccharomyces pombe as a model organism for studies of chromosome segreration

Cited by 2 publications

References 25 publications

High-throughput transcriptome sequencing and comparative analysis of Escherichia coli and Schizosaccharomyces pombe in respiratory and fermentative growth

High-throughput transcriptome sequencing and comparative analysis of Escherichia coli and Schizosaccharomyces pombe in respiratory and fermentative growth

Design, synthesis, pharmacological evaluation and DNA interaction studies of binuclear Pt(II) complexes with pyrazolo[1,5‐a]pyrimidine scaffold

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