Schistosome transcriptome: insights and perspectives for functional genomics

Verjovski-Almeida, Sérgio; Leite, Luciana C. C.; Dias-Neto, Emmanuel; Menck, C. F. M.; Wilson, R. A.

doi:10.1016/j.pt.2004.04.012

Cited by 44 publications

(23 citation statements)

References 22 publications

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“…In 2003 the transcriptomes of S. mansoni (Verjovski-Almeida et al 2003) and S. japonicum (Hu et al 2003) were published in Nature Genetics, giving insights and perspectives for functional genomics (Verjovski-Almeida et al 2004). Six years later, in 2009, the genome sequences from both parasites were published in Nature (Berriman et al 2009, Zhou et al 2009).…”

Section: The Genome and Transcriptome Of Schistosomesmentioning

confidence: 99%

Non-coding RNAs in schistosomes: an unexplored world

Oliveira

Carvalho

Maracaja-Coutinho

et al. 2011

An. Acad. Bras. Ciênc.

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Non-coding RNAs (ncRNAs) were recently given much higher attention due to technical advances in sequencing which expanded the characterization of transcriptomes in different organisms. ncRNAs have different lengths (22 nt to >1,000 nt) and mechanisms of action that essentially comprise a sophisticated gene expression regulation network. Recent publication of schistosome genomes and transcriptomes has increased the description and characterization of a large number of parasite genes. Here we review the number of predicted genes and the coverage of genomic bases in face of the public ESTs dataset available, including a critical appraisal of the evidence and characterization of ncRNAs in schistosomes. We show expression data for ncRNAs in Schistosoma mansoni. We analyze three different microarray experiment datasets: (1) adult worms' large-scale expression measurements; (2) differentially expressed S. mansoni genes regulated by a human cytokine (TNF-α) in a parasite culture; and (3) a stage-specific expression of ncRNAs. All these data point to ncRNAs involved in different biological processes and physiological responses that suggest functionality of these new players in the parasite's biology. Exploring this world is a challenge for the scientists under a new molecular perspective of host-parasite interactions and parasite development.

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Section: The Genome and Transcriptome Of Schistosomesmentioning

confidence: 99%

Non-coding RNAs in schistosomes: an unexplored world

Oliveira

Carvalho

Maracaja-Coutinho

et al. 2011

An. Acad. Bras. Ciênc.

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“…On another front, identification of potential chemotherapeutic targets for existent drugs or rational-drug design has recently been pushed forward by the publication of the S. mansoni (Verjovski-Almeida et al 2003) and S. japonicum (Hu et al 2003) transcriptomes and the launching of functional-genomic and proteomic analyses (McManus et al 2004;Ashton et al 2001;Verjovski-Almeida et al 2004;Fitzpatrick et al 2005).…”

Section: Identification Of New Chemotherapeutic Targetsmentioning

confidence: 99%

Schistosomiasis—a century searching for chemotherapeutic drugs

2006

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Schistosomiasis affects 200 million individuals in underdeveloped and developing regions and is a growing concern for travelers worldwide. There has been evidence of resistance to the praziquantel-based therapy and reports of acute-disease manifestation; therefore, other drugs affecting different stages of the schistosome parasites life cycle and alternative therapeutic regimens should be developed and become accessible. The present review results from a comprehensive search in the scientific literature for substances and compounds tested in the past centennial for schistosomiasis therapy. We gathered over 40 drugs providing information on therapeutic action in humans or animal model, toxicity, susceptible Schistosoma stages, species, etc. The drugs were grouped according to their known metabolic effects on the parasite, whether they are on membrane structure and function, carbohydrate metabolism, protein synthesis and function, or on nucleic acid metabolism. We discuss the current knowledge of drug-target interactions, their mechanism of action and possible therapy combinations. Furthermore, based in the literature and in our own experience with large-scale Schistosoma mansoni genome and transcriptome analyses, we put forward several recently described gene products that are promising target candidates for existing or new drugs.

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“…In the pre-genome era of schistosome research, this approach was used and yielded a large number of new genes (Franco et al, 1995;Oliveira, 2001) cDNA clones were also widely distributed to the research community. More recently, larger scale projects on S. mansoni have immensely expanded the knowledge and made mining the information much more effective (Oliveira and Johnston, 2001;Hu et al, 2003;VerjovskiAlmeida et al, 2003;Verjovski-Almeida et al, 2004;Oliveira, unpublished effort of the RGMG). For S. japonicum one large project was published .…”

mentioning

confidence: 99%

The Schistosoma mansoni transcriptome: An update

Oliveira¹

2007

Experimental Parasitology

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Large scale EST sequencing projects have been carried out for S. mansoni and S. japonicum. This update will briefly review the most recent accomplishments in the area and discuss the use of EST data for the purposes of gene discovery, gene model development, genome annotation and SNP analysis. In addition, the use of ESTs for studying other features of the transcriptome such as splice site and transcription initiation variants will be discussed as well as approaches to assigning function to unknown transcripts. Although EST sequencing has contributed much for schistosome research, other data mining possibilities exist, including the identification of putative drug and vaccine targets.

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Schistosome transcriptome: insights and perspectives for functional genomics

Cited by 44 publications

References 22 publications

Non-coding RNAs in schistosomes: an unexplored world

Non-coding RNAs in schistosomes: an unexplored world

Schistosomiasis—a century searching for chemotherapeutic drugs

The Schistosoma mansoni transcriptome: An update

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