Schistosoma mansoni: Continuous Variation in Susceptibility of the Vector Snail of Schistosomiasis, Biomphalaria tenagophila I. Self-Fertilization—Lineage

Mascara, Douglas; Kawano, Toshie; Magnanelli, Antonio Carlos; Silva, Rosângela P.S.; ́Anna, Osvaldo Augusto Sant; Morgante, João Stenghel

doi:10.1006/expr.1999.4444

Cited by 7 publications

(5 citation statements)

References 30 publications

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“…Most of the studies on variation of snail susceptibility were interested in experimental selection of host stocks yielding either 0% or approaching 100% infection frequencies when exposed to only one particular strain of schistosome (Mascara et al, 1999;Richards et al, 1992;Webster and Woolhouse, 1998). As we know that a host strain un-susceptible to one parasite strain could be totally susceptible to another one, such an approach gives limited information about the real efficiency level of its defense system against the diversity of…”

Section: Selection Of Multi-parasite (Un)-susceptible Host Phenotypesmentioning

confidence: 99%

Multi-parasite host susceptibility and multi-host parasite infectivity: A new approach of the Biomphalaria glabrata/Schistosoma mansoni compatibility polymorphism

Thèron

Rognon

Gourbal

et al. 2014

Infection, Genetics and Evolution

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In this study, we analyze the degree of susceptibility/un-susceptibility of five strains of Biomphalaria glabrata from different geographical origins successively challenged with a panel of 4 Schistosoma mansoni strains. A total of 20 homopatric and heteropatric host-parasite combinations were tested with exposure doses of 1, 10, 20, 30 and 50 miracidia per individual host. By doing this, we characterized each B. glabrata strain by its "multi-parasite susceptibility phenotype" that reflects better the efficiency of their defense mechanism against not only one, but a diversity of schistosome stocks. In the same time, all the S. mansoni strains used were characterized, by their "multi-host infectivity phenotype" that reflects the level of infectivity they display when confronted to diverse snail populations. Based on these results it is possible to select different homogenous stocks of snails with different spectrum of susceptibility/un-susceptibility for several parasite strains. This will be a useful tool for future functional studies conducted to understand the genetics and molecular basis of the compatibility polymorphism in this host/parasite model.

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Section: Selection Of Multi-parasite (Un)-susceptible Host Phenotypesmentioning

confidence: 99%

Multi-parasite host susceptibility and multi-host parasite infectivity: A new approach of the Biomphalaria glabrata/Schistosoma mansoni compatibility polymorphism

Thèron

Rognon

Gourbal

et al. 2014

Infection, Genetics and Evolution

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show abstract

“…The schistosomiasis vector mollusks, genus Biomphalaria, show different levels of susceptibility to infection by Schistosoma mansoni (Ruiz, 1957;Corrêa, 1963, 1978;Mascara et al, 1999). Susceptibility may vary in relation to geographic areas, the local population, individuals, and individuals of different ages (Richards, 1984).…”

Section: Introductionmentioning

confidence: 99%

Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection

Oliveira

Levada²,

Zanotti-Magalhães³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. The relationships between schistosomiasis and its intermediate host, mollusks of the genus Biomphalaria, have been a concern for decades. It is known that the vector mollusk shows different susceptibility against parasite infection, whose occurrence depends on the interaction between the forms of trematode larvae and the host defense cells. These cells are called amebocytes or hemocytes and are responsible for the recognition of foreign bodies and for phagocytosis and cytotoxic reactions. The defense cells mediate the modulation of the resistant and susceptible phenotypes of the mollusk. Two main types of hemocytes are found in the Biomphalaria hemolymph: the granulocytes and the hyalinocytes. We studied the variation in the number (kinetics) of hemocytes for 24 h after exposing the parasite to genetically selected and non-selected strains of 2437 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (4): 2436-2445 (2010) Differences in the number of hemocytes in B. tenagophila Biomphalaria tenagophila, susceptible or not to infection by Schistosoma mansoni. The differences were analyzed referred to the variations in the number of hemocytes in mollusks susceptible or not to infection by S. mansoni. The hemolymph of the selected and non-selected snails was collected, and hemocytes were counted using a Neubauer chamber at six designated periods: 0 h (control, non-exposed individuals), 2 h, 6 h, 12 h, 18 h and, 24 h after parasite exposure. Samples of hemolymph of five selected mollusks and five non-selected mollusks were separately used at each counting time. There was a significant variation in the number of hemocytes between the strains, which indicates that defense cells have different behaviors in resistant and susceptible mollusks.

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“…Thus, the resulting phenotype would be determined by several genes in the young mollusk and by only one gene in the adult. According to Mascara et al (1999), the resistant phenotype would be the outcome of a great number of alleles present in a small number of loci or the outcome of few alleles in multiple loci in "linkage". The resisting phenotype would be the result of an "alteration" (change) in any of these different loci related to the susceptibility phenotype.…”

Section: Introductionmentioning

confidence: 99%

Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis

Oliveira¹,

Silva²,

Manzano³

et al. 2010

Genet. Mol. Res.

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ABSTRACT. Despite the implementation control programs, schistosomiasis continues to spread throughout the world. Among modern control strategies, vector control is currently being emphasized. Within this context, analysis of the genetic variability of intermediate host snails (Biomphalaria spp) is important because it allows identification of specific sequences of the genome of this mollusk related to suscep- 1451©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 9 (3): 1450-1459 (2010) Genetic variability between Biomphalaria glabrata strains tibility/resistance to Schistosoma mansoni infection. We investigated Brazilian albino (non-pigmented) and pigmented (wild type) strains of Biomphalaria glabrata; these strains differ in their susceptibility to S. mansoni infection. Genetic variability was studied by RAPD-PCR using different random primers. The electrophoretic patterns resulting from amplification showed specific polymorphic markers for the albino and pigmented strains of B. glabrata. This information will help in the identification and isolation of genes specifically related to susceptibility, demonstrating that RAPD-PCR is an appropriate and efficient methodological approach for analysis of the genetic variability of schistosomiasis vectors.

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Schistosoma mansoni: Continuous Variation in Susceptibility of the Vector Snail of Schistosomiasis, Biomphalaria tenagophila I. Self-Fertilization—Lineage

Cited by 7 publications

References 30 publications

Multi-parasite host susceptibility and multi-host parasite infectivity: A new approach of the Biomphalaria glabrata/Schistosoma mansoni compatibility polymorphism

Multi-parasite host susceptibility and multi-host parasite infectivity: A new approach of the Biomphalaria glabrata/Schistosoma mansoni compatibility polymorphism

Differences in the number of hemocytes in the snail host Biomphalaria tenagophila, resistant and susceptible to Schistosoma mansoni infection

Genetic differences between strains of Biomphalaria glabrata (Planorbidae) that are susceptible and unsusceptible to schistosomiasis

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