Schisandrin B protects against LPS-induced inflammatory lung injury by targeting MyD88

Zhu, Wenzhen; Luo, Wei; Han, Jibo; Zhang, Qiuyan; Ji, Lijun; Самородов, Александр В.; Павлов, В. Н.; Zhuang, Zaishou; Yang, Daona; Yin, Lilin; Li-jiang, Huang; Liang, Guang; Huh, Joo Young; Wang, Yi

doi:10.1016/j.phymed.2022.154489

Cited by 14 publications

(4 citation statements)

References 44 publications

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“…Foreign inflammation-inducing substances such as LPS can initiate the downstream NF-κB/MAPK inflammatory cascade by binding to the transmembrane protein TLR4 on the cell surface, which promotes gene transcription and protein expression of inflammation-related factors, resulting in the development of the body’s inflammatory response [ 39 ]. The time-course data indicated that FLs blocked the activation of TLR4 from 0.5 h ( Figure 4 ).…”

Section: Resultsmentioning

confidence: 99%

The Anti-Inflammatory Mechanism of Flaxseed Linusorbs on Lipopolysaccharide-Induced RAW 264.7 Macrophages by Modulating TLR4/NF-κB/MAPK Pathway

Jing

Huang³

et al. 2023

Foods

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Flaxseed linusorbs (FLs), cyclic peptides derived from flaxseed oils, have shown multiple activities such as anticancer, antibacterial, and anti-inflammatory effects. However, the anti-inflammatory monomers of FLs and their mechanisms are still unclear. In this study, we have elucidated that FLs suppress the modulation of NF-κB/MAPK signaling pathways by targeting the inhibition of activating TLR4 in LPS-induced RAW 264.7 cells. Therefore, the transcription and expression of inflammatory cytokines (i.e., TNF-α, IL-1β, and IL-6) and inflammatory mediator proteins (i.e., iNos and Cox-2) were significantly suppressed by FLs. In addition, an in silico study discovered that eight monomers of FLs showed high-affinity bindings with TLR4. In silico data combined with HPLC results indicated that FLA and FLE, accounting for 44%, were likely the major anti-inflammatory monomers in FLs. In summary, FLA and FLE were proposed as the main anti-inflammatory active cyclopeptides via hindering TLR4/NF-κB/MAPK signaling pathways, suggesting the potential use of food-derived FLs as natural anti-inflammatory supplements in a daily diet.

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Section: Resultsmentioning

confidence: 99%

The Anti-Inflammatory Mechanism of Flaxseed Linusorbs on Lipopolysaccharide-Induced RAW 264.7 Macrophages by Modulating TLR4/NF-κB/MAPK Pathway

Jing

Huang³

et al. 2023

Foods

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“…LPS is an important factor in triggering pneumonia and has been widely studied for its potential to cause endothelial barrier dysfunction; it can effectively activate immune cells via TLR-4 and induce a variety of pro-inflammatory mediators and cytokines through different signaling pathways [6,31]. The inflammation mediation is closely related to the TLR-4/MyD88/MAPK signaling pathway [32,33]. New anti-inflammatory drugs are being discovered based on the inhibition of the TLR-4/MyD88/MAPK signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways

Liu,

Zhang,

Feng

et al. 2024

IJMS

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Acute lung injury (ALI) is a serious inflammatory disease with high morbidity and mortality. Rosavin is an anti-inflammatory and antioxidant phenylpropanoid and glucoside, which is isolated from Rhodiola rosea L. However, its potential molecular mechanisms and whether it has protective effects against lipopolysaccharide (LPS)-induced ALI remain to be elucidated. To assess the in vitro anti-inflammatory effects and anti-lung injury activity of rosavin, RAW264.7 and A549 cells were stimulated using 1 μg/mL LPS. Rosavin attenuated LPS-induced activation of the TLR-4/NF-κB signaling pathway in RAW264.7 cells and inhibited LPS-induced release of inflammatory factors in A549 cells. A mouse model of acute lung injury was constructed by intraperitoneal injection of 5 mg/kg LPS to observe the therapeutic effect of rosavin. Transcriptomics analysis and Western blot assays were utilized to verify the molecular mechanism, rosavin (20, 40, and 80 mg/kg) dose-dependently ameliorated histopathological alterations, reduced the levels of inflammatory factors, and inhibited the TLR-4/NF-κB/MAPK signaling pathway and apoptosis activation. Rosavin is a promising therapeutic candidate for acute lung injury by inhibiting the TLR-4/NF-κB/MAPK pathway.

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“…Prior investigations have indicated that the induction of TLR4 leads to the modulation of pro-inflammatory cytokine expression, which is primarily achieved via MyD88-dependent or MyD88-independent signaling cascades [ 24 ]. TLR4 can interact with MyD88 to stimulate TAK1, resulting in the initiation of NF-κB and MAPK signaling cascades that regulate pro-inflammatory factor ( Tnf-α, Ccl4, Ccl3 ) expression [ 25 ]. Moreover, TLR4 binding to TRIF induces activation of TBK1, thereby prompting IRF3 activation and the subsequent production of pro-inflammatory cytokines ( Cxcl9, Cxcl10, Il-11 ) [ 26 ].…”

Section: Discussionmentioning

confidence: 99%

Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response

Lan,

Wang,

Huang

et al. 2023

Nat. Prod. Bioprospect.

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Quzhou Aurantii Fructus (QAF) has a long history as a folk medicine and food for the treatment of liver diseases. While our earlier study provided evidence of hepatoprotective properties contained within the flavonoids and limonins constituents in QAF, the potential preventative effects afforded by essential oil components present within QAF remains enigmatic. In this study, we prepared Quzhou Aurantii Fructus essential oil (QAFEO) and confirmed its anti-inflammatory effects on liver inflammation through experimentation on lipopolysaccharide and D-galactosamine (LPS/D-GalN) induced acute liver failure (ALF) mouse models. Using RNA-sequence (RNA-seq) analysis, we found that QAFEO prevented ALF by systematically blunting the pathways involved in response to LPS and toll-like receptor signaling pathways. QAFEO effectively suppressed the phosphorylation of tank-binding kinase 1 (TBK1), TGF-beta activated kinase 1 (TAK1), interferon regulatory factor 3 (IRF3), and the activation of mitogen activated kinase-like protein (MAPK) and nuclear factor-kappa B (NF-κB) pathways in vivo and in vitro. Importantly, QAFEO substantially reduced myeloid differentiation primary response gene 88 (MyD88)- toll-like receptor 4 (TLR4) interaction levels. Moreover, 8 compounds from QAFEO could directly bind to REAL, TAK1, MyD88, TBK1, and IRF3. Taken together, the results of our study support the notion that QAFEO exerts a hepatoprotective effect through inhibiting LPS-mediated inflammatory response. Graphical abstract

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Schisandrin B protects against LPS-induced inflammatory lung injury by targeting MyD88

Cited by 14 publications

References 44 publications

The Anti-Inflammatory Mechanism of Flaxseed Linusorbs on Lipopolysaccharide-Induced RAW 264.7 Macrophages by Modulating TLR4/NF-κB/MAPK Pathway

The Anti-Inflammatory Mechanism of Flaxseed Linusorbs on Lipopolysaccharide-Induced RAW 264.7 Macrophages by Modulating TLR4/NF-κB/MAPK Pathway

Rosavin Alleviates LPS-Induced Acute Lung Injure by Modulating the TLR-4/NF-κB/MAPK Singnaling Pathways

Essential oil extracted from Quzhou Aurantii Fructus prevents acute liver failure through inhibiting lipopolysaccharide-mediated inflammatory response

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