SCFAs-Induced GLP-1 Secretion Links the Regulation of Gut Microbiome on Hepatic Lipogenesis in Chickens

Zhang, Jian Mei; Sun, Yin; Zhao, Li; Chen, Tian Tian; Fan, Mei Na; Jiao, Hong; Zhao, Jing; Wang, Xiao Juan; Li, Fu Chang; Li, Hai Fang; Lin, Hai

doi:10.3389/fmicb.2019.02176

Cited by 51 publications

(42 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies have reported that propionate would stimulate PYY and GLP-1 secretion from intestinal L cells ( Kaji et al., 2011 ), thereby inhibiting appetite and further reducing fat accumulation in mammals ( Chambers et al., 2015 ; Psichas et al., 2015 ). Our recent study ( Zhang et al., 2019 ) also found propionate induces GLP-1 secretion in cultured intestinal epithelial cells, and GLP-1 suppresses hepatocytic lipogenesis in vitro. The appetite-suppressing peptides might be involved in the inhibition of oral propionate administration on chicken fat deposition, as the average daily feed intake of broilers was significantly reduced.…”

Section: Discussionmentioning

confidence: 68%

“…Our findings fit well with previous reports in which oral administration or colonic infusion of SP could reduce fat accumulation and weight gain in mice and humans ( Chambers et al., 2015 ; Song et al., 2019 ). Alteration of gut microbiota after dietary treatments has been established to play a key role in regulating fat metabolism ( Wu et al., 2019 ; Zhang et al., 2019 ). To test whether dietary supplementation of SP suppressed chicken fat deposition via gut microbiota, we performed high-throughput sequencing on hypervariable region of the 16S rRNA genes of cecal bacteria.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Propionate inhibits fat deposition via affecting feed intake and modulating gut microbiota in broilers

Zhao

Liu

et al. 2021

Poultry Science

Self Cite

View full text Add to dashboard Cite

As one of the 3 main short-chain fatty acids, the role of propionate in chicken fat metabolism is largely unknown. In this study, we demonstrated that dietary supplementation of coated sodium propionate ( SP ) moderately inhibits fat deposition in broiler chickens, as evidenced by the decreased adipocyte mean area ( P < 0.01), the lowered triglyceride content in abdominal fat tissue ( P < 0.01), and the reduced transcription of several lipogenic genes in liver and abdominal fat tissues ( P < 0.05). Surprisingly, the propionate content was not significantly elevated either in serum or in the cecal chyme by SP administration ( P > 0.05). However, SP application significantly decreased the average daily feed intake of broilers ( P < 0.05). In addition, the composition of the cecal microbial communities was altered, with the ratio of Firmicutes to Bacteroidetes decreasing in particular ( P < 0.05). At the genus level, SP application increased the richness of Alistipes , Lactobacillus , and Bifidobacterium , while reduced the abundance of Lachnospiraceae and Helicobacter significantly ( P < 0.05). Moreover, in vitro experiments indicated that, although physiological concentrations of propionate (0.01 to 0.1 mmol) upregulated or downregulated the transcription of some fat synthesis-associated genes ( P < 0.05), they did not significantly affect the triglyceride accumulation in hepatocytes and adipocytes ( P > 0.05). These results suggest that feed supplementation with SP inhibits fat deposition in broilers by reducing feed and caloric intake, but not via direct regulation on hepatic fat synthesis or adipocytic fat deposition. Alteration in the relative populations of the gut microflora suggests that SP may have gut health implications.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 99%

Propionate inhibits fat deposition via affecting feed intake and modulating gut microbiota in broilers

Zhao

Liu

et al. 2021

Poultry Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…It was reported that SCFAs produced by the microbiota upregulated the expression of GLP-1 via activation of the MAPK pathway. GLP-1 induced reduction in mRNA expression of the fatty acid synthesis related genes [37][38][39]. Therefore, it is suggested that SCFA production by soy protein intake may be related to a reduction in fatty acid synthesis related gene expression.…”

Section: Discussionmentioning

confidence: 99%

The Timing Effects of Soy Protein Intake on Mice Gut Microbiota

et al. 2019

View full text Add to dashboard Cite

Soy protein intake is known to cause microbiota changes. While there are some reports about the effect of soy protein intake on gut microbiota and lipid metabolism, effective timing of soy protein intake has not been investigated. In this study, we examined the effect of soy protein intake timing on microbiota. Mice were fed twice a day, in the morning and evening, to compare the effect of soy protein intake in the morning with that in the evening. Mice were divided into three groups: mice fed only casein protein, mice fed soy protein in the morning, and mice fed soy protein in the evening under high-fat diet conditions. They were kept under the experimental condition for two weeks and were sacrificed afterward. We measured cecal pH and collected cecal contents and feces. Short-chain fatty acids (SCFAs) from cecal contents were measured by gas chromatography. The microbiota was analyzed by sequencing 16S rRNA genes from feces. Soy protein intake whether in the morning or evening led to a greater microbiota diversity and a decrease in cecal pH resulting from SCFA production compared to casein intake. In addition, these effects were relatively stronger by morning soy protein intake. Therefore, soy protein intake in the morning may have relatively stronger effects on microbiota than that in the evening.

show abstract

“… 74 C. butyricum supplementation increased GLP-1 secretion from the L cells within the intestinal epithelium, putatively due to increased butyrate production triggering the activation of G-protein-coupled receptors 41 and 43 (GPR41 and GPR43). 20 , 71 , 75 Therefore, modulation of GLP-1 signaling may be a mechanism by which C. butyricum CGMCC 9831 in the gut exerts anti-apoptotic neuroprotection in the model of traumatic brain injury 19 , 38 , 70 ( Figure 4a ). Similarly, in a model of chronic stress-induced depression, C. butyricum CGMCC 9830 administration reversed depression-like behavior, increased levels of hippocampal BDNF, and also increased intestinal levels of GLP-1.…”

Section: Butyricum Supplementation: Mechanisms Of Action and Target Pathwaysmentioning

confidence: 99%

Butyrate-producing human gut symbiont, Clostridium butyricum, and its role in health and disease

Stoeva¹,

Garcia-So²,

Justice³

et al. 2021

Gut Microbes

206

156

View full text Add to dashboard Cite

Clostridium butyricum is a butyrate-producing human gut symbiont that has been safely used as a probiotic for decades. C. butyricum strains have been investigated for potential protective or ameliorative effects in a wide range of human diseases, including gut-acquired infection, intestinal injury, irritable bowel syndrome, inflammatory bowel disease, neurodegenerative disease, metabolic disease, and colorectal cancer. In this review we summarize the studies on C. butyricum supplementation with special attention to proposed mechanisms for the associated health benefits and the supporting experimental evidence. These mechanisms center on molecular signals (especially butyrate) as well as immunological signals in the digestive system that cascade well beyond the gut to the liver, adipose tissue, brain, and more. The safety of probiotic C. butyricum strains appears well-established. We identify areas where additional human randomized controlled trials would provide valuable further data related to the strains' utility as an intervention.

show abstract

SCFAs-Induced GLP-1 Secretion Links the Regulation of Gut Microbiome on Hepatic Lipogenesis in Chickens

Cited by 51 publications

References 61 publications

Propionate inhibits fat deposition via affecting feed intake and modulating gut microbiota in broilers

Propionate inhibits fat deposition via affecting feed intake and modulating gut microbiota in broilers

The Timing Effects of Soy Protein Intake on Mice Gut Microbiota

Butyrate-producing human gut symbiont, Clostridium butyricum, and its role in health and disease

Contact Info

Product

Resources

About