SCD1 is nutritionally and spatially regulated in the intestine and influences systemic postprandial lipid homeostasis and gut-liver crosstalk

Gut Microbiota Remodeling and Intestinal Adaptation to Lipid Malabsorption After Enteroendocrine Cell Loss in Adult Mice

“…For example, transcripts for the fatty acid transporter Slc27a1 /FATP1 and the thyroid hormone-responsive gene Thrsp regulating de novo lipogenesis 22 were increased in the duodenum and ileum. Other genes involved in lipogenesis ( Scd1 ) and cholesterol metabolism ( Pcsk9 , Ldlr ) were specifically upregulated in the duodenum 22 , 23 , 24 ( Figure 4 A, D ). The most significant fold change in the ileum was observed for Pla2g4c, which was strongly increased in EEC-deficient mice (Log2FC = 6.4; adjusted P value = 1.36E‒20).…”

Dietary oleic acid contributes to the regulation of food intake through the synthesis of intestinal oleoylethanolamide

“…Stearoyl-CoA desaturase 1 (SCD1) is the rate limiting enzyme that catalyzes the biosynthesis of monounsaturated fatty acids from saturated fatty acids ( 26 ). SCD1 is expressed at high levels in the liver and adipose tissue but is also expressed in other tissues, including the intestine ( 27 , 28 ). Although SCD1 is expressed in the intestine at relatively low levels, it plays important roles in the synthesis of OA in the enterocytes and the maintenance of fatty acid homeostasis ( 15 , 27 ).…”

“…SCD1 is expressed at high levels in the liver and adipose tissue but is also expressed in other tissues, including the intestine ( 27 , 28 ). Although SCD1 is expressed in the intestine at relatively low levels, it plays important roles in the synthesis of OA in the enterocytes and the maintenance of fatty acid homeostasis ( 15 , 27 ). Conditional SCD1–knockout mice with knockout in the intestinal epithelium exhibited inflammation and tumor burden in the gut, but OA feeding reversed these effects ( 15 ).…”

Retinoic acid regulates pyruvate dehydrogenase kinase 4 (Pdk4) to modulate fuel utilization in the adult heart: Insights from wild‐type and β‐carotene 9′,10′ oxygenase knockout mice

“…Heart (LV) lipid extracts were also prepared by Folch extraction 45 to be analyzed by TLC GC–MS as described previously 46,47 . Briefly, tissue extracts were dissolved in chloroform, 2% Triton X100 solution and subsequently evaporated under nitrogen and dissolved in water.…”

“…Heart (LV) lipid extracts were also prepared by Folch extraction 45 to be analyzed by TLC GC-MS as described previously. 46,47 Briefly, tissue extracts were dissolved in chloroform, 2% Triton X100 solution and subsequently evaporated under nitrogen and dissolved in water. Lipids were then separated by thin-layer chromatography (TLC) on silica gel-60 plates (Millipore Sigma, St. Louis, MO, USA) in heptane-isopropyl ether-glacial acetic acid (60:40:3, v/v/v) with authentic standards.…”