Scavenging of Active Oxygen Species by Glycated Proteins

Okamoto, Genichi; Hayase, Fumitaka; Kato, Harubumi

doi:10.1271/bbb.56.928

Cited by 58 publications

(26 citation statements)

References 3 publications

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“…2. DPPH radical scavenging activity in the extract, which was the same conclusion in the other studies (Pourali et al, 2010;Wiboonsirikul et al, 2007). In addition the other substances, which were contributed to the activity, were browning reaction products including protein-carbohydrate conjugates, melanoidins and heterocyclic compounds (Okai and HigashiOkai, 2006;Xu et al, 2001;Friedman, 1996;Okamoto et al, 1992). Although the temperatures were raised from 160 to 250℃ at a 6.25 mL/g-bran of amount of fed water, the activity hardly increased, which was similar to the slight change in the cumulative amount of phenolic substances at the same amount of water used in Fig.…”

Section: Resultssupporting

confidence: 81%

Effects of Temperature and Flow Rate on Subcritical-water Extraction from Defatted Rice Bran

Viriya‐empikul

Wiboonsirikul

Kobayashi

et al. 2012

FSTR

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The effect of the subcritical water treatment along with the combination of static and dynamic modes on the recovery of protein, carbohydrate and phenolic substances from defatted rice bran was investigated at various treatment temperatures of 120, 160, 200 and 250℃ and the flow rates of 0.5, 2.5 and 5 mL/ min. The highest amounts of protein and carbohydrate were recovered at 160℃ and that of the phenolic substances was recovered at 250℃. Around half of the protein and carbohydrate contents were degraded at 200 and 250℃. The various flow rates from 0.5 to 5 mL/min did not significantly affect the contents of the protein, carbohydrate and phenolic substances recovered from the bran. The extract also had a DPPH radical scavenging activity, which depended on the treatment temperature. While the extract obtained at 120℃ at the same flow rate and same amount of water fed to the bran had the lowest radical scavenging activity, the activities in the extracts at 160 − 250℃ were around 3 times higher than that at 120℃ and there was no significant difference among them.Keywords: defatted rice bran, sub-critical water extraction, radical scavenging activity *To whom correspondence should be addressed. E-mail: adachi@kais.kyoto-u.ac.jp IntroductionDefatted rice bran, which has a very low price, is typically used as animal feed or fertilizer for mushroom cultivation. Indeed, the defatted rice bran can increase its value by recovering valuable substances, such as ferulic acid, a substance exhibiting antioxidative and UV-absorbing properties, and tocopherol (Cuvelier et al., 1992;Castelluccio et al., 1995;Packer, 1991). In addition to the ferulic acid, the bran also contains other phenolic substances including p-coumaric, p-hydroxybenzoic, vanillic, protocatechuic, syringic, caffeic and gentisic acids in significant amounts as well as high protein and carbohydrate contents (Pourali et al., 2010;Okai and Higashi-Okai, 2006). These substances, some of which would be bound to bran matrix, can be liberated and recovered from the defatted rice bran by organic solvent, subcritical water, and supercritical carbon dioxide extractions (Wiboonsirikul et al., 2008;Iqbal et al., 2005;Kim et al., 1999).Subcritical water is hot water at the temperature range from 100 to 374℃ under pressurized conditions in order to maintain its liquid state. Regarding its environmental concern and time requirement, the subcritical water treatment is more advantageous than other methods to recover beneficial biomaterials from agricultural products (Iqbal et al., 2005;Kim et al., 1999;Chittapalo and Noomhorm, 2009). The unique properties of the subcritical water include a lower dielectric constant or polarity and higher ion product at higher temperatures. The dielectric constant of hot water at 250℃ is close to those of some organic solvents such as methanol, acetone and ethanol and the concentrations of the hydrogen and hydroxyl ions at this temperature are higher by three orders of magnitude than that in ambient water. Thus, the subcritical water can recove...

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Section: Resultssupporting

confidence: 81%

Effects of Temperature and Flow Rate on Subcritical-water Extraction from Defatted Rice Bran

Viriya‐empikul

Wiboonsirikul

Kobayashi

et al. 2012

FSTR

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“…The mechanism of free radical scavenging by polysaccharides is still not fully understood. It is only known that glycolated-protein may scavenge ROS by one electron transfer or a hydrogen abstraction mechanism (Okamoto et al, 1992). It has also been reported that the functional activity of polysaccharide could be influenced by many factors such as molecular weight, branched degree, water solubility, structure and configuration (Yanaki et al, 1986;Bohn and BeMiller, 1995).…”

Section: Antioxidant Activities In Relation To Protein Polysaccharidmentioning

confidence: 99%

Retracted: Tissue culture of some lichens and screening of their antioxidant, antityrosinase and antibacterial properties

Behera

Verma

Sonone

et al. 2007

Phytotherapy Research

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Lichen species Usnea ghattensis, Heterodermia podocarpa, Arthothelium awasthii and Parmotrema tinctorum have been cultured in vitro and screened for their antioxidant, antimicrobial and antityrosinase potential using different assay systems. The methanol extract of lichens showed antioxidant, antimicrobial and antityrosinase activities according to the order Usnea ghattensis > Arthothelium awasthii > Heterodermia podocarpa > Parmotrema tinctorum. The IC(50) values for the antioxidant and antityrosinase activities of U. ghattensis and A. awasthii were less than or equivalent to that of standard antioxidants and tyrosinase inhibitors. The methanol extracts of the lichen cultures Usnea ghattensis and Arthothelium awasthii were effective against Bacillus licheniformis, Bacillus megaterium, Bacillus subtilis and Staphylococcus aureus. The minimum inhibitory concentration (MIC) of the extract was found to be between 5 and 10 microg extract/mL. The results suggested that the extract of lichen cultures, U. ghattensis and A. awasthii, could be of use as an easily accessible source of natural antioxidants, tyrosinase inhibitory and antimicrobial properties as possible food supplements or in the pharmaceutical industry.

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“…The possibility of the protein-carbohydrate conjugate acting as a radical scavenger has been suggested in some studies. 37,38) The activity increased when amino acids or the protein were heated in the presence of reducing sugars. Since the rice bran is rich in hemicellulose, the radical scavenging activity of the extract may also have resulted from the degradation of hemicellulose to oligosaccharides, monomeric sugars and sugar-degradation products (furfural and hydroxymethylfurfural) which were also involved in the Maillard browning reaction.…”

mentioning

confidence: 99%

Production and Characterization of Functional Substances from a By-Product of Rice Bran Oil and Protein Production by a Compressed Hot Water Treatment

Wiboonsirikul¹,

Kimura²,

Kanaya³

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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Scavenging of Active Oxygen Species by Glycated Proteins

Cited by 58 publications

References 3 publications

Effects of Temperature and Flow Rate on Subcritical-water Extraction from Defatted Rice Bran

Effects of Temperature and Flow Rate on Subcritical-water Extraction from Defatted Rice Bran

Retracted: Tissue culture of some lichens and screening of their antioxidant, antityrosinase and antibacterial properties

Production and Characterization of Functional Substances from a By-Product of Rice Bran Oil and Protein Production by a Compressed Hot Water Treatment

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