Scavenging effect of schizandrins on active oxygen radicals

Abstract

The reactive oxygen radicals produced from human polymorphonuclear leukocytes (PMN) stimulated with PMA (phorbol myristate acetate), hydroxyl radicals generated by a Fenton reaction, and superoxide anion radicals produced by irradiating solutions of riboflavin in the presence of EDTA have been taken as the models for production of oxygen radicals. With the use of the electron spin resonance spin trapping method, the scavenging effects of schizandrol A (solA) (5 x 10(-4) M) and schizandrin B (sinB) (5 x 10(-4) M) have been studied and compared with the effects of vitamin E (5 x 10(-4) M) and vitamin C (5 x 10(-4) M). It has been found that in cell system the scavenging effects of sinB and solA, as judged by ESR spin trappings, on hydrpxyl radicals (.OH) are greater than vitamin E and vitamin C and the scavenging effects on superoxide anion (O2) are greater than vitamin E but lower than vitamin C. With respect to the Fenton reaction, sinB has the strogest scavenging effect on .OH (77%) and solA has strong scavenging effect on .OH (63%), both of them larger than that of vitamin E (35%) and vitamin C (56%). In the riboflavin/EDTA system, the scavenging effect of sinB (46%) is smaller than that of vitamin C (96%) but larger than that of vitamin E (23%); the scavenging effect of solA is not obvious (14%). With the use of spin probe oximetry, the oxygen consumption during the respiratory burst of stimulated PMN has been measured when exposed to schizandrins. The experiment results demonstrated that they do not affect the activity of production of active oxygen radicals in the respiratory burst of PMN stimulated with PMA.

Shengmai San reduces hepatic lipids and lipid peroxidation in rats fed on a high-cholesterol diet

“…Several studies have suggested that the antioxidant activities of SMS may contribute to some of its therapeutic effects (Ko et al, 2002;Wang et al, 2003;You et al, 2006). In the present study, ginsenoside Rg 2 (13.7 g/g) and schizandrin (20.5 g/g), the two abundant components found in SMS, have been shown to possess significant antioxidant activity (Zhao et al, 1990;Lu et al, 2001). These results suggested that, in addition to hepatic lipid-lowering effect, the anti-oxidative effect of SMS might reduce the depletion of hepatic GSH and reduce oxidative stress, resulting in a lower lipid peroxidation in liver.…”

Protective Effects and Active Ingredients of Yi-Qi-Fu-Mai Sterile Powder Against Myocardial Oxidative Damage in Mice

“…It was also found that lignan compounds exerted beneficial effects on the cardiovascular system (41). Schizandrol A was an effective scavenger for oxygen radicals such as superoxide anion and hydroxyl radical (42). Schisandrin B can enhance antioxidant capacity of heart tissue by significantly increasing the activity of glutathione peroxidase, glutathione reductase, glutathione transferase, and SOD (43).…”

Dibenzo[a,c]cyclooctadiene lignans of the genus Schisandra: importance, isolation and determination

“…Their activity seems to be comparable to that of vitamin E, especially in liver microsomes where schisanhenol was most potent [49]. Free radical scavenging acitivity (FRSA) of schisandrol A and schisandrin B for the hydroxyl radical and superoxide anion was compared with that of vitamin E and C. Their efficiency for the hydroxyl radical was higher than that of the two vitamins, for the superoxide anion they were more potent than vitamin E [50]. According to literature FRSA against oxygen radicals is dependent on the configuration of biphenyls; Iignans with S-configuration have better activity than those with R-configuration [51].…”