Scavenging capacity of superoxide radical and screening of antimicrobial activity of Castanea sativa Mill. extracts

Abstract

AbstractŽivković J., Zeković Z., Mujić I., Vidović S., Cvetković D., Lepojević Ž., Nikolić G., Trutić N. (2010): Scavenging capacity of superoxide radical and screening of antimicrobial activity of Castanea sativa Mill. extracts. Czech J. Food Sci., 28: 61-68.The superoxide radical scavenging and antimicrobial activity were examined of sweet chestnuts: seeds, peeled chestnut, brown seed coat, leaves, catkin, spiny burs, and chestnut bark as well as of Lovran's marrone leaves and grafted Italian marrone cultivar. Parts of chestnut were extracted under the same conditions with 50% ethanol as extractant and afterwards the dry extracts of the examined samples were obtained. The total phenolics and flavonoids contents were determined using standard spectrophotometrical methods. The capacity of the investigated extracts to remove radicals was evaluated by EPR method. The most efficient in scavenging• O 2 − radicals proved to be the extracts of leaves of grafted Italian marrone (RI = 86%) and of Lovran's marrone cultivar (RI = 80%). Catkin, leaves, chestnut bark, and spiny burs extracts demonstrated the highest antimicrobial activity. Very significant and significant correlations were established between the antimicrobial activity of extracts and • O 2 − radicals scavenging in all samples examined. The extracts of Castanea Sativa Mill. are important sources of components active in reducing the level of oxidative stress.

Components responsible for antimicrobial activity of propolis from continental and Mediterranean regions in Croatian

“…Taken together, the Spearman rank-order correlations between total phenol and flavonoid and DPPH and FRAP indicated that phenolic compounds have a more important role than flavonoids in determining scavenging capacity and antioxidant power. Similar observations have been made with Turkish propolis: propolis with the greatest phenol and flavonoid content exhibited the most pronounced antioxidant activity (Popova et al 2005;Živković et al 2010). Thus, our results support the hypothesis of a high correlation between the antioxidant activity and phenolics and flavonoids.…”

Sweet Chestnut (Castanea sativaMill.) Bark Extract: Cardiovascular Activity and Myocyte Protection against Oxidative Damage

“…Based on the literature data [28–31], gallic acid was selected as reference standard for the TP content by Folin-Ciocalteu spectrophotometric method. TP content of the examined CSM bark extract was 54.9% of dry weight (g GAE/100 g of extract).…”

Antioxidant and Antimicrobial Activities of Ethanol Extracts of <i>Cynara Scolymus (Cynarae folium, Asteraceae</i> Family)

“…Pearson correlation test was conducted to determine correlations between the variables. Significant level was set at p ≤ 0.05 [16].…”

“…There is a direct relation between antioxidant activity and the reducing power. Furthermore, the direct relationship between reducing power and antioxidant activity has also been correlated with extract concentration and extraction method [6,16].…”