Scavenging Capacity of Extracts of Arrabidaea chica Leaves from the Amazonia against ROS and RNS of Physiological and Food Relevance

Abstract

Arrabidaea chica, a medicinal plant found in the Amazon rainforest, is a promising source of bioactive compounds which can be used to inhibit oxidative damage in both food and biological systems. In this study, the in vitro scavenging capacity of characterized extracts of A. chica leaves, obtained with green solvents of different polarities [water, ethanol, and ethanol/water (1:1, v/v)] through ultrasound-assisted extraction, was investigated against reactive oxygen (ROS) and nitrogen (RNS) species, namely superoxide anion radicals (O2•−), hydrogen peroxide (H2O2), hypochlorous acid (HOCl), and peroxynitrite anion (ONOO−). The extract obtained with ethanol–water presented about three times more phenolic compound contents (11.8 mg/g) than ethanol and water extracts (3.8 and 3.6 mg/g, respectively), with scutellarein being the major compound (6.76 mg/g). All extracts showed high scavenging efficiency against the tested ROS and RNS, in a concentration-dependent manner with low IC50 values, and the ethanol–water extract was the most effective one. In addition, all the extracts were five times more efficient against ROO• than Trolox. Therefore, the extracts from A. chica leaves exhibited high promising antioxidant potential to be used against oxidative damage in food and physiological systems.

Development of a Deep Eutectic Solvent-Assisted Kaempferol Hydrogel: A Promising Therapeutic Approach for Psoriasis-like Skin Inflammation

“…Xanthine oxidase is responsible for catalyzing the xanthine and xanthine oxidase reaction systems in the organism, leading to the production of superoxide anion radicals. 31 These results imply that the DK-pGEL hydrogel has the potential to attenuate or delay the progression of reactive species associated with oxidative stress in vitro. At lower concentrations, DK 10 -pGEL exhibited a higher efficacy in scavenging free radicals within the body.…”

“…This effect could be attributed to the ability of KAE to capture O 2 •– radicals or to inhibit the enzyme xanthine oxidase. Xanthine oxidase is responsible for catalyzing the xanthine and xanthine oxidase reaction systems in the organism, leading to the production of superoxide anion radicals . These results imply that the DK-pGEL hydrogel has the potential to attenuate or delay the progression of reactive species associated with oxidative stress in vitro.…”

“…The DK-pGEL hydrogel containing KAE acts as an antioxidant to eliminate superoxide radicals. Its scavenging effect was determined by monitoring the changes in absorbance at 550 nm over a specified period of time . Each group was tested three times.…”

“…Its scavenging effect was determined by monitoring the changes in absorbance at 550 nm over a specified period of time. 31 Each group was tested three times. The scavenging effect was determined by eq 4:…”

Engenharia de Pesca: o avanço da ciência no Brasil

“…Como resultado, o uso de produtos naturais é usado em estudos fitoterápicos, promovendo ganhos para fins de ornamentação (TAVECHIO et al, 2009;OLIVEIRA et al, 2017). As ervas têm sido usadas com o objetivo de favorecer a subsistência do ambiente e a presciência de doenças, além de ajudar a melhorar o bem-estar animal (SIQUEIRA, 2019). Na maioria dos casos, as propriedades curativas das plantas estão relacionadas à sua atividade antioxidante (SÜNTAR et al, 2012).…”