Scarless Fetal Mouse Wound Healing May Initiate Apoptosis Through Caspase 7 and Cleavage of PARP

Carter, Richard; Sykes, Virginia W.; Lanning, David A.

doi:10.1016/j.jss.2009.03.074

Cited by 21 publications

(15 citation statements)

References 14 publications

(21 reference statements)

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“…The presence of cleaved PARP is a commonly used diagnostic tool for the detection of apoptosis. 35 The western blot results in Figure 5 show the expression of such apoptotic markers in the lanes with curcumin-liposomes-treated KHOS. Cleaved caspases and cleaved PARP were not observed in lane 1 containing lysate of untreated cells.…”

Section: Resultsmentioning

confidence: 98%

Curcumin-loaded γ-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma

Dhule

Penfornis

Frazier

et al. 2012

Nanomedicine: Nanotechnology, Biology and Medicine

271

138

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The delivery of curcumin, a broad-spectrum anticancer drug, has been explored in the form of liposomal nanoparticles to treat osteosarcoma (OS). Curcumin is water insoluble and an effective delivery route is through encapsulation in cyclodextrins followed by a second encapsulation in liposomes. Liposomal curcumin’s potential was evaluated against cancer models of mesenchymal (OS) and epithelial origin (breast cancer). The resulting 2-Hydroxypropyl-γ-cyclodextrin/curcumin - liposome complex shows promising anticancer potential both in vitro and in vivo against KHOS OS cell line and MCF-7 breast cancer cell line. An interesting aspect is that liposomal curcumin initiates the caspase cascade that leads to apoptotic cell death in vitro in comparison with DMSO-curcumin induced autophagic cell death. In addition, the efficiency of the liposomal curcumin formulation was confirmed in vivo using a xenograft OS model. Curcumin-loaded γ-cyclodextrin liposomes indicate significant potential as delivery vehicles for the treatment of cancers of different tissue origin. From the Clinical Editor Curcumin-loaded γ-cyclodextrin liposomes were demonstrated in vitro to have significant potential as delivery vehicles for the treatment of cancers of mesenchymal and epithelial origin. Differences between mechanisms of cell death were also evaluated.

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Section: Resultsmentioning

confidence: 98%

Curcumin-loaded γ-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma

Dhule

Penfornis

Frazier

et al. 2012

Nanomedicine: Nanotechnology, Biology and Medicine

271

138

View full text Add to dashboard Cite

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“…Cleavage of Casp7 and PARP were significantly increased in scarless fetal wound healing (embryonic day 15) compared to fetal wound that resulted in a scar (embryonic day 18). 20 Similar to oral mucosal wound healing, regeneration or improved scarring outcomes have been identified in fetal wound healing; several observations suggest potential reasons for the similarities. First, similar to oral wound healing, the immune response in fetal wound healing is significantly lower than adult wound healing.…”

Section: Discussionmentioning

confidence: 95%

“…34 Lastly, similar to oral wound healing, the angiogenic response in fetal wound healing is significantly lower than adult wound healing. 1 Given the numerous similarities between fetal and oral wound healing, the finding of differences in the levels and mechanisms of apoptosis in both oral and fetal wounds 20 suggests that apoptosis may play a significant role in the determination of scarring outcomes.…”

Section: Discussionmentioning

confidence: 99%

Differential Apoptosis in Mucosal and Dermal Wound Healing

Johnson

Francis

DiPietro

2014

Advances in Wound Care

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“…The active caspase-9 recruits and activates procaspase-3, generating a fragment that activates the mitochondrial pathway. The DNA repair enzyme poly(ADP)-ribose polymerase (PARP) is shown to be cleaved by caspase-3 and as a result becomes incapable of responding to DNA damage during apoptosis [6,7].…”

Section: Introductionmentioning

confidence: 99%

Carnosic Acid, a Rosemary Phenolic Compound, Induces Apoptosis Through Reactive Oxygen Species-Mediated p38 Activation in Human Neuroblastoma IMR-32 Cells

Tsai

Lin

et al. 2011

Neurochem Res

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Carnosic acid (CA), a rosemary phenolic compound, has been shown to display anti-cancer activity. We examined the apoptotic effect of CA in human neuroblastoma IMR-32 cells and elucidated the role of the reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK) associated with carcinogenesis. The result indicated that CA decreased the cell viability in a dose-dependent manner. Further investigation in IMR-32 cells revealed that cell apoptosis following CA treatment is the mechanism as confirmed by flow cytometry, hoechst 33258, and caspase-3/-9 and poly(ADP-ribose) polymerase (PARP) activation. Immunoblotting suggested a down-regulation of anti-apoptotic Bcl-2 protein in the CA-treated cells. In flow cytometric analysis, CA caused the generation of reactive oxygen species (ROS); however, pretreatment with the antioxidant N-acetylcysteine (NAC) attenuated the CA-induced generation of ROS and apoptosis. This effect was accompanied by increased activation of p38 and by decreased activation of extracellular signal-regulated kinase (ERK) as well as activation of c-Jun NH(2)-terminal kinase (JNK). Moreover, NAC attenuated the CA-induced phosphorylation of p38. Silencing of p38 by siRNA gene knockdown reduced the CA-induced activation of caspase-3. In conclusion, ROS-mediated p38 MAPK activation plays a critical role in CA-induced apoptosis in IMR-32 cells.

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Scarless Fetal Mouse Wound Healing May Initiate Apoptosis Through Caspase 7 and Cleavage of PARP

Cited by 21 publications

References 14 publications

Curcumin-loaded γ-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma

Curcumin-loaded γ-cyclodextrin liposomal nanoparticles as delivery vehicles for osteosarcoma

Differential Apoptosis in Mucosal and Dermal Wound Healing

Carnosic Acid, a Rosemary Phenolic Compound, Induces Apoptosis Through Reactive Oxygen Species-Mediated p38 Activation in Human Neuroblastoma IMR-32 Cells

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