Scanning tunneling microscopy, atomic force microscopy, and related techniques

Snyder, Shelly R.; White, Henry S.

doi:10.1021/ac00036a006

Cited by 28 publications

(4 citation statements)

References 521 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in spite of the fact that AFM provides increased power of magnification/resolution coupled to real-time imaging of living samples-capabilities not shared with any other single imaging modality. AFM also provides the investigator with an unequaled measurement capability for minute samples (Snyder & White, 1992), permitting precise computation of surface areas, volumes, and linear distance.…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional imaging of living and dying neurons with atomic force microscopy

McNally

Borgens

2004

J Neurocytol

View full text Add to dashboard Cite

Atomic Force Microscopy (AFM) has been used to image the morphology of developing neurons and their processes. Additionally, AFM can physically interact with the cell under investigation in numerous ways. Here we use the AFM to both three-dimensionally image the neuron and to inflict a nano/micro-puncture to its membrane. Thus, the same instrument used as a tool to precisely penetrate/cut the membrane at the nanoscale level is employed to image the morphological responses to damage. These first high resolution AFM images of living chick dorsal root ganglion cells and cells of sympathetic ganglion and their growing processes provide confirmation of familiar morphologies. The increased resolution of the AFM revealed these structures to be significantly more complex and variable than anticipated. Moreover we describe novel, dynamic, and unreported architectures, particularly large dorsally projecting ridges, spines, and ribbons of cytoplasm that appear and disappear on the order of minutes. In addition, minute (ca. 100 nm) hair-like extensions of membrane along the walls of nerve processes that also shift in shape and density, appearing and disappearing over periods of minutes were seen. We also provide "real time'' images of the death of the neuron cell body after nano/micro scale damage to its membrane. These somas excreted their degraded cytoplasm, revealed as an enlarging pool beneath and around the cell. Conversely, identical injury, even repeated perforations and nanoslices, to the neurite's membrane do not lead to demise of the process. This experimental study not only provides unreported neurobiology and neurotrauma, but also emphasizes the unique versatility of AFM as an instrument that can (1) physically manipulate cells, (2) provide precise quantitative measurements of distance, surface area and volume at the nanoscale if required, (3) derive physiologically significant data such as membrane pressure and compliance, and (4) during the same period of study-provide unexcelled imaging of living samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Three-dimensional imaging of living and dying neurons with atomic force microscopy

McNally

Borgens

2004

J Neurocytol

View full text Add to dashboard Cite

show abstract

“…Dissolution and crystallization processes that occur at solid/liquid interfaces are of key importance in a wide variety of chemical reactions . Scanning probe microscopy has provided unprecedented kinetic and structural information on the elementary steps involved in these processes, particularly on the roles of atomic level surface features such as terrace, ledge, and kink sites . Scanning tunnelling microscopy (STM) and atomic force microscopy (AFM) have been used to investigate dissolution reactions at both electrically conducting − and insulating surfaces. − When the substrate is conducting, dissolution can be potentiostatically controlled by connecting the sample as an electrode and monitoring topographical surface reactivity as a function of the applied potential or current. − For insulating surfaces, precise control of the interfacial undersaturation, which promotes the dissolution process, is more difficult to achieve.…”

Section: Introductionmentioning

confidence: 99%

In-Situ Imaging of Ionic Crystal Dissolution Using an Integrated Electrochemical/AFM Probe

et al. 1996

View full text Add to dashboard Cite

The kinetics and mechanism controlling dissolution from the (100) cleavage face of potassium bromide single crystals in acetonitrile solutions have been identified using a novel integrated electrochemical/AFM probe and a scanning electrochemical microscope (SECM). With both techniques, dissolution is induced by perturbing the dynamic dissolution/growth equilibrium at the crystal/solution interface through the electrochemical oxidation of bromide ions. SECM measurements demonstrate that the dissolution reaction is diffusion-limited under the experimental conditions, suggesting that the surface reaction is characterized by a rate constant in excess of 5 cm s-1 (assuming a first-order dissolution process). The topography of the dissolving surface has been imaged in situ, under conditions which closely mimic those of the SECM measurements, using an electrochemically active AFM probe. These studies provide the first direct experimental evidence of the operation of the spiral mechanism in the dissolution of an ionic single crystal, in which steps of unit cell height unwind from screw dislocations emerging on the crystal surface.

show abstract

“…The scanning probe microscopies have proven invaluable as surface characterization tools to an impressive range of fundamental and technological areas. − We have, in parallel with the efforts of others, − been exploring the use of the friction and adhesion modes of scanning force microscopy (SFM) as probes of the compositional transformations of interfaces at nanometer length scales. , While continuing such investigations, we have discovered that the mechanical interaction between a SFM probe tip and various ester-functionalized alkanethiolate monolayers can notably accelerate the rate of the base hydrolysis of the ester linkage in small, spatially defined locations. The following describes our initial findings using monolayers chemisorbed at Au(111) from dithiobis(succinimido undecanoate) (DSU).…”

Section: Introductionmentioning

confidence: 99%

SFM Tip-Assisted Hydrolysis of a Dithiobis(succinimido undecanoate) Monolayer Chemisorbed on a Au(111) Surface

et al. 1997

View full text Add to dashboard Cite

This paper reports on the use of a scanning force microscope (SFM) for the tip-assisted base hydrolysis of an ester-terminated alkanethiolate monolayer on Au(111). We have found that contact imaging accelerates the base hydrolysis of a dithiobis(succinimido undecanoate) monolayer relative to the surrounding unimaged area. It is proposed that (1) the mechanical disruption by the SFM probe tip of the steric barrier imposed by the neighboring adsorbates facilitates access of hydroxide ions to the buried acyl carbons in the adlayer, and (2) the surface area hydrolytically transformed by this disruption can be controlled by the SFM imaging conditions. Findings in support of our conclusions are presented, and potential implications to nanotechnology are briefly discussed.

show abstract

Scanning tunneling microscopy, atomic force microscopy, and related techniques

Cited by 28 publications

References 521 publications

Three-dimensional imaging of living and dying neurons with atomic force microscopy

Three-dimensional imaging of living and dying neurons with atomic force microscopy

In-Situ Imaging of Ionic Crystal Dissolution Using an Integrated Electrochemical/AFM Probe

SFM Tip-Assisted Hydrolysis of a Dithiobis(succinimido undecanoate) Monolayer Chemisorbed on a Au(111) Surface

Contact Info

Product

Resources

About