Scanning Thermal Microscopy

Ocola, Leonidas E.

doi:10.1002/0471266965.com154

Cited by 1 publication

(2 citation statements)

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“…13 (4) Scanning thermal probe: The temperature sensor of a scanning thermal probe is applied to image the surface temperature of a single cell at the macroand nanometer levels. 14 (5) Temperature sensor based on thermistors: The real-time temperature of the population cell was reflected by detecting the change in the resistance of the temperature-sensitive resistor. 15 The abovementioned methods can detect cell temperature, but each has its own limitations.…”

Section: ■ Introductionmentioning

confidence: 99%

“…(3) Thermocouple probe: A thermocouple probe can detect the temperature change of a single cell with high precision by converting the temperature signal into an electromotive force signal . (4) Scanning thermal probe: The temperature sensor of a scanning thermal probe is applied to image the surface temperature of a single cell at the macro- and nanometer levels . (5) Temperature sensor based on thermistors: The real-time temperature of the population cell was reflected by detecting the change in the resistance of the temperature-sensitive resistor .…”

Section: Introductionmentioning

confidence: 99%

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Combined Cellular Thermometry Reveals That Salmonella typhimurium Warms Macrophages by Inducing a Pyroptosis-like Phenotype

Chen

Chang

et al. 2022

J. Am. Chem. Soc.

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The attenuated Salmonella typhimurium VNP20009, enriched in tumors, is known to have antitumor effects and recruit macrophages. Little is known, however, about whether VNP will lead to specific changes in macrophages, e.g., cell temperature. Here, using a real-time wireless multicell thermometry system, we reported for the first time that VNP20009 increases the macrophage temperature by 0.2 °C. Nigericin, recognized as an inducer of pyroptosis, was found to induce macrophage warming. Moreover, the ΔsipD-VNP20009 strain failed to induce macrophage pyroptosis and simultaneously failed to warm macrophages, and the Gsdmd −/− macrophages that were unable to achieve pyroptosis were no longer warmed following VNP20009 induction. These results suggested that the occurrence of macrophage pyroptosis is the key to VNP20009-mediated cell warming. With the aid of a single-cell thermometry system, it was further confirmed that cell warming occurred in pyroptosis-like macrophages. Cellular warming was not detected after the induction of pyroptosis in macrophages with loss of mitochondrial biological function, suggesting a critical role of mitochondria in warming. Moreover, we found that VNP20009 caused local tumor temperature increases. The local tumor warming induced by VNP20009 was significantly reduced after macrophage clearance. Notably, this temperature increase contributed to M1-type polarization. These findings expanded our knowledge of the cellular biological changes induced by the strain on macrophages, as well as the biochemical phenomena accompanying pyroptosis, and provide a reference for the study of biochemical signals transduced to biothermal signals with a combined cell-level temperature detector.

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