Scanning Super-Resolution Imaging in Enclosed Environment by Laser Tweezer Controlled Superlens

Wen, Yangdong; Yu, Haibo; Zhao, Wenxiu; Pan, Li; Wang, Feifei; Ge, Zhixing; Wang, Xiaoduo; Liu, Lianqing; Li, Wen J.

doi:10.1016/j.bpj.2020.10.032

Cited by 13 publications

(4 citation statements)

References 40 publications

(39 reference statements)

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“…Li et al achieved stable and controllable image scanning of samples using chemical dynamics to drive the microsphere lens [132]. In addition, various attempts have been made to improve the field of view of microspheres in super-resolution imaging and achieve large-area imaging in a controllable position [133,134]. Krivitsky et al achieved sample imaging of gold split squares deposited on silicon substrates with 73 nm gaps using a micropipette for accurate positioning between the squares [135], as shown in Figure 7d.…”

Section: Super-resolution Imaging By Photonic Nanojets 41 Optical Imaging Of Nanostructures With Movable Microspheresmentioning

confidence: 99%

Optical Trapping, Sensing, and Imaging by Photonic Nanojets

Song

Zhao

et al. 2021

Photonics

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The optical trapping, sensing, and imaging of nanostructures and biological samples are research hotspots in the fields of biomedicine and nanophotonics. However, because of the diffraction limit of light, traditional optical tweezers and microscopy are difficult to use to trap and observe objects smaller than 200 nm. Near-field scanning probes, metamaterial superlenses, and photonic crystals have been designed to overcome the diffraction limit, and thus are used for nanoscale optical trapping, sensing, and imaging. Additionally, photonic nanojets that are simply generated by dielectric microspheres can break the diffraction limit and enhance optical forces, detection signals, and imaging resolution. In this review, we summarize the current types of microsphere lenses, as well as their principles and applications in nano-optical trapping, signal enhancement, and super-resolution imaging, with particular attention paid to research progress in photonic nanojets for the trapping, sensing, and imaging of biological cells and tissues.

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Section: Super-resolution Imaging By Photonic Nanojets 41 Optical Imaging Of Nanostructures With Movable Microspheresmentioning

confidence: 99%

Optical Trapping, Sensing, and Imaging by Photonic Nanojets

Song

Zhao

et al. 2021

Photonics

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“…However, these studies had limited detection areas due to static microsphere imaging or fixed microsphere conditions. By incorporating a dynamic scanning element, such as an AFM cantilever 29,30 , mechanical stage movements 31,32 , optical tweezer methods 33 , or acoustofluidics 34,35 , both high resolution and a large field of view can be achieved. Among these methods, acoustofluidic manipulation is advantageous because it has a programable process, vast particle size manipulation range, and contactless manipulation nature [36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53] .…”

Section: Introductionmentioning

confidence: 99%

Acoustofluidic scanning fluorescence nanoscopy with a large field of view

Jin,

Upreti,

Rich

et al. 2024

Microsyst Nanoeng

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Large-field nanoscale fluorescence imaging is invaluable for many applications, such as imaging subcellular structures, visualizing protein interactions, and high-resolution tissue imaging. Unfortunately, conventional fluorescence microscopy requires a trade-off between resolution and field of view due to the nature of the optics used to form the image. To overcome this barrier, we developed an acoustofluidic scanning fluorescence nanoscope that simultaneously achieves superior resolution, a large field of view, and strong fluorescent signals. The acoustofluidic scanning fluorescence nanoscope utilizes the superresolution capabilities of microspheres that are controlled by a programmable acoustofluidic device for rapid fluorescence enhancement and imaging. The acoustofluidic scanning fluorescence nanoscope resolves structures that cannot be resolved with conventional fluorescence microscopes with the same objective lens and enhances the fluorescent signal by a factor of ~5 without altering the field of view of the image. The improved resolution realized with enhanced fluorescent signals and the large field of view achieved via acoustofluidic scanning fluorescence nanoscopy provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine, biology, biophysics, and biomedical engineering.

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“… 28 However, these studies have a limited detection area due to the static microsphere imaging or fixed microsphere condition. By incorporating a dynamic scanning element, such as an AFM cantilever, 29 , 30 mechanical stage movements, 31 , 32 optical tweezer methods, 33 or acoustofluidics, 34 , 35 both high resolution and a large field of view can be achieved. Of these aforementioned methods, acoustofluidic manipulation is advantageous due to its programable process, vast particle size manipulation range, and contactless manipulation nature.…”

Section: Introductionmentioning

confidence: 99%

Acoustofluidic scanning fluorescence nanoscopy with large field of view

Huang

Jin

Rich

et al. 2023

Preprint

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Nanoscale fluorescence imaging with a large-field view is invaluable for many applications such as imaging of subcellular structures, visualizing protein interaction, and high-resolution tissue imaging. Unfortunately, conventional fluorescence microscopy has to make a trade-off between resolution and field of view due to the nature of the optics used to form an image. To overcome this barrier, we have developed an acoustofluidic scanning fluorescence nanoscope that can simultaneously achieve superior resolution, a large field of view, and enhanced fluorescent signal. The acoustofluidic scanning fluorescence nanoscope utilizes the super-resolution capability of microspheres that are controlled by a programable acoustofluidic device for rapid fluorescent enhancement and imaging. The acoustofluidic scanning fluorescence nanoscope can resolve structures that cannot be achieved with a conventional fluorescent microscope with the same objective lens and enhances the fluorescent signal by a factor of ~5 without altering the field of view of the image. The improved resolution with enhanced fluorescent signal and large field of view via the acoustofluidic scanning fluorescence nanoscope provides a powerful tool for versatile nanoscale fluorescence imaging for researchers in the fields of medicine, biology, biophysics, and biomedical engineering.

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Scanning Super-Resolution Imaging in Enclosed Environment by Laser Tweezer Controlled Superlens

Cited by 13 publications

References 40 publications

Optical Trapping, Sensing, and Imaging by Photonic Nanojets

Optical Trapping, Sensing, and Imaging by Photonic Nanojets

Acoustofluidic scanning fluorescence nanoscopy with a large field of view

Acoustofluidic scanning fluorescence nanoscopy with large field of view

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