Scanning electron microscopy study of Tritrichomonas augusta

Borges, Fernanda Pires; Wiltuschnig, Renata C. M.; Tasca, Tiana; Carli, Geraldo A De

doi:10.1007/s00436-004-1189-2

Cited by 9 publications

(9 citation statements)

References 14 publications

(15 reference statements)

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“…Ruffled cells accounted for up to 90% of the cell population; however, time interval experiments showed an increase in the number of smooth cells at 72 h. There was no statistically significant difference in size between the two morphological forms. While ruffled cells are observed in other trichomonads, it is unknown what significance this change in trophozoite surface structure represents (59). While the smooth cell types observed were initially postulated to represent a pseudocystlike stage, experiments to induce pseudocyst formation using adverse environmental growth conditions failed (7).…”

Section: Morphological Characteristics Trophozoitesmentioning

confidence: 99%

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

et al. 2016

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SUMMARYDientamoeba fragilisis a protozoan parasite of the human bowel, commonly reported throughout the world in association with gastrointestinal symptoms. Despite its initial discovery over 100 years ago, arguably, we know less about this peculiar organism than any other pathogenic or potentially pathogenic protozoan that infects humans. The details of its life cycle and mode of transmission are not completely known, and its potential as a human pathogen is debated within the scientific community. Recently, several major advances have been made with respect to this organism's life cycle and molecular biology. While many questions remain unanswered, these and other recent advances have given rise to some intriguing new leads, which will pave the way for future research. This review encompasses a large body of knowledge generated on various aspects ofD. fragilisover the last century, together with an update on the most recent developments. This includes an update on the latest diagnostic techniques and treatments, the clinical aspects of dientamoebiasis, the development of an animal model, the description of aD. fragiliscyst stage, and the sequencing of the firstD. fragilistranscriptome.

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Section: Morphological Characteristics Trophozoitesmentioning

confidence: 99%

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

et al. 2016

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“…internalize flagella to form pseudocysts. In previous studies using SEM, we also demonstrated the morphology of Trichomonas gallinae and Tritrichomonas augusta and the occurrence of pseudocysts (Tasca and De Carli 2003;Borges et al 2004). True cyst wall is present in few trichomonad species, such as those found in the intestinal trichomonads Trichomonas batrachorum and Trichomitus sanguisugae (Brugerolle 1973) and in the free-living trichomonad Ditrichomonas honigbergii (Farmer 1993).…”

Section: Resultsmentioning

confidence: 99%

“…Pseudocyst formation appears under experimental stress conditions, probably indicating a defense mechanism of the parasite, and it also occurs under natural culture conditions (Granger et al 2000;Pereira-Neves et al 2003). Pseudocysts can be seen in cultures maintained under standard conditions but suffering a stress at some point, such as pH alteration or nutrients depletion, leading the parasite to undergo pseudocyst formation (Borges et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Morphological aspects of Monocercomonas sp. and investigation on probable pseudocysts occurrence

et al. 2007

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Monocercomonas sp. is a flagellate protozoan found in the large intestine of snakes and in insects. Light microscopy revealed the measurements of morphological features of the trophozoites. Scanning electron microscopy showed in detail the emergence of the three anterior flagella, the recurrent flagellum, the axostyle, and the absence of undulating membrane. In addition, we described spherical forms which are probably pseudocysts. The investigation on the occurrence of this process was carried out through the incubation of Monocercomonas sp. trophozoites in several stressful conditions, such as pH change, nutrient depletion and different temperatures. Results revealed high pseudocyst formation at acidic pH values (4.0, 5.0, and 6.0), in absence of serum and in incubation at 37 degrees C. The occurrence of these pseudocystic forms in trichomonads life cycle is under investigation. This study describes the external structure of Monocercomonas sp., as demonstrated by light and scanning electron microscopy. Moreover, to our knowledge, this is the first time that formation of probable pseudocysts is shown in Monocercomonas sp., contributing to the research field on termite protozoa biology.

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“…Cells were fixed according to Borges et al (2004) and the grids examined by a scanning electron microscope (model XL-30, Philips). Gram staining was performed (Doetsch, 1981) and motility verified by the SIM (Hydrogen-Sulfide, Indole, Motility; Mac Faddin, 2000) test.…”

Section: Morphology Motility and Electron Microscopymentioning

confidence: 99%

Isolation and characterization of two plant growth-promoting bacteria from the rhizoplane of a legume (Lupinus albescens) in sandy soil

Giongo

Beneduzi

Ambrosini

et al. 2010

Rev. Bras. Ciênc. Solo

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SUMMARYTwo bacterial strains that amplified part of the nifH gene, RP1p and RP2p, belonging to the genus Enterobacter and Serratia, were isolated from the rhizoplane of Lupinus albescens. These bacteria are Gram-negative, rod-shaped, motile, facultative anaerobic, and fast-growing; the colonies reach diameters of 3-4 mm within 24 h of incubation at 28 °C. The bacteria were also able to grow at temperatures as high as 40 °C, in the presence of high (2-3 % w/v) NaCl concentrations and pH 4 -10. Strain RP1p was able to utilize 10 of 14 C sources, while RP2p utilized nine. The isolates produced siderophores and indolic compounds, but none of them was able to solubilize phosphate. Inoculation of L. albescens with RP1p and RP2p strains resulted in a significant increase in plant dry matter, indicating the plant-growth-promoting abilities of these bacteria. Index terms: indolic compounds, siderophores, inoculation experiment.(1) Recebido para publicação em setembro de 2008 e aprovado em janeiro de 2010.

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Scanning electron microscopy study of Tritrichomonas augusta

Cited by 9 publications

References 14 publications

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel

Morphological aspects of Monocercomonas sp. and investigation on probable pseudocysts occurrence

Isolation and characterization of two plant growth-promoting bacteria from the rhizoplane of a legume (Lupinus albescens) in sandy soil

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