Scanning electron microscopy of immobilized bacteria in gel beads: a comparative study of fixation methods

Neerven, A.R.W. van; Wijffels, R.H.; Zehnder, A.J.B.

doi:10.1016/0167-7012(90)90052-8

Cited by 36 publications

(18 citation statements)

References 21 publications

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“…1, SEM micrographs show the κ‐carrageenan gel just after inoculation under different magnifications. In agreement with the literature (Van Neerven et al. , 1990), the gel matrix clearly exhibited a compact internal structure with a pore size smaller than 1 μm.…”

Section: Methodssupporting

confidence: 92%

Listeria growth under diffusional limitations in synthetic meats

Noriega¹,

Laca²,

Dı́az³

2009

Int J of Food Sci Tech

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Physicochemical properties of meat products make them susceptible to contamination by several pathogens such as Listeria monocytogenes. As reference of L. monocytogenes behaviour, the evolution of Listeria innocua growth was monitored at different positions within a j-carrageenan synthetic meat and visualised by scanning electron microscopy (SEM). Microbial growth was also determined in a liquid medium, prepared with the same nutrients as the solid phase, and the effect of oxygen concentration, a fundamental parameter in meat preservation, was studied. To simulate microbial behaviour in the solid, results of kinetics in broth were incorporated to a diffusion-reaction model that considers growth kinetics as a function of the local oxygen concentration. Data from the model have been then discussed, taking into account experimental data and SEM observations. This model, useful for food safety purposes, has been shown to provide a reasonably good simulation of experimental data in the structured synthetic meat.

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Section: Methodssupporting

confidence: 92%

Listeria growth under diffusional limitations in synthetic meats

Noriega¹,

Laca²,

Dı́az³

2009

Int J of Food Sci Tech

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“…Stabilization of carbohydrate hydrogel systems such as the one described in this work is difficult using conventional sample preparation and microscopy (Van Neerven, Wijffels, & Zehnder, 1990). Conventional sample preparation methods rely on the exposure of the samples to protein crosslinking fixatives such as glutaraldehyde which are applied in an aqueous based buffer.…”

Section: Resultsmentioning

confidence: 99%

Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation

Allan‐Wojtas

Hansen

Paulson

2008

LWT - Food Science and Technology

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“…Immobilized or free yeast cells were dehydrated and prepared for observation with a scanning electron microscope according to a standard procedure described by Van Neerven et al [16].…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

Biodegradation of polyphenols with immobilized Candida tropicalis under metabolic induction

Ettayebi

Errachidi

Jamai

et al. 2003

FEMS Microbiology Letters

100

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During olive oil production, large quantities of olive mill wastewater (OMW) are produced. This wastewater material, containing a high level of phenolic compounds, poses a serious environmental problem in almost all Mediterranean countries. Candida tropicalis YMEC14 was used as an extremophile strain to design an aerobic biotreatment process to detoxify OMW and reduce its polluting organic load. The process was enhanced by directing yeast metabolism towards biodegradation pathways using hexadecane as co-metabolite and by immobilizing yeast cells in calcium alginate beads. Under immobilization conditions, C. tropicalis YMEC14 grown at 40 ‡C in OMW supplemented with hexadecane resulted in 69.7%, 69.2% and 55.3% reduction of chemical oxygen demand, monophenols and polyphenols, respectively, after a 24-h fermentation cycle.

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Scanning electron microscopy of immobilized bacteria in gel beads: a comparative study of fixation methods

Cited by 36 publications

References 21 publications

Listeria growth under diffusional limitations in synthetic meats

Listeria growth under diffusional limitations in synthetic meats

Microstructural studies of probiotic bacteria-loaded alginate microcapsules using standard electron microscopy techniques and anhydrous fixation

Biodegradation of polyphenols with immobilized Candida tropicalis under metabolic induction

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