Scanning electron microscopy of experimental adiaspiromycosis

Hejtmánek, M.

doi:10.1007/bf00707178

Cited by 4 publications

(2 citation statements)

References 3 publications

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“…Surveys have indicated that adiaspiromycosis is found commonly in the white-footed mouse (Peromyscus m n iculatus) in western Canada (Dowding, 1947;Bakerspigel, 1956). Ultrastructural studies of adiaspiromycosis in experimental animals and a naturally infected human have been reported (Malinsky et al, 1972;Watts et al, 1975;Hejtmanek, 1976). The present report describes light and electron microscopic findings in lungs and lymph nodes of striped skunks naturally infected with Emmonsiu sp.…”

Section: Methodsmentioning

confidence: 53%

Adiaspiromycosis in Striped Skunks in Alberta, Canada

Albassam

Bhatnagar

Lillie

et al. 1986

Journal of Wildlife Diseases

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Pulmonary adiaspiromycosis was diagnosed in seven of 25 striped skunks (Mephitis mephitis) in east-central Alberta. The infection varied from mild, where only microscopic lesions were seen, to severe, where gross lesions of grayish-white nodules were observed in the lung parenchyma. Mild lesions were restricted to the lung, while severe lesions extended to the tracheobronchial and mediastinal lymph nodes. Histologically, the lesions were characterized by a centrally located fungal spherule, surrounded by granulomatous inflammation. The morphology of the fungal spherules was consistent with that of Emmonsiu crescens. By electron microscopy, the fungal cells had an outer thick fibrillar wall and an inner cytoplasm filled with large lipid vacuoles with relatively few mitochondria, ribosomes or glycogen inclusions. The absence of endosporulation and budding suggested that each fungal cell in the lung represented a separate inhaled spore. Infection was by inhalation, nevertheless adiaspores may disseminate to the regional lymph nodes.

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Section: Methodsmentioning

confidence: 53%

Adiaspiromycosis in Striped Skunks in Alberta, Canada

Albassam

Bhatnagar

Lillie

et al. 1986

Journal of Wildlife Diseases

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“…An inhalation model has been used with rabbits [ 12 ] to analyze humoral immunological responses. Experimental mouse models of adiaspiromycosis using intraperitoneal infection routes have been described in the literature [ 4 , 13 , 14 ]. The models used neither an intranasal nor an intratracheal route.…”

Section: Introductionmentioning

confidence: 99%

Adiaspore development and morphological characteristics in a mouse adiaspiromycosis model

Takeshige

Nakano

Kondoh

et al. 2020

Vet Res

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Lesions of adiaspiromycosis, a respiratory disease affecting wild animals, have been found mainly in dead mammals and free-living mammals captured for surveillance. No report has described an investigation of adiaspore formation progress in the lung. After establishing an experimental mouse model of intratracheal adiaspiromycosis infection with the causative agent Emmonsia crescens, we observed adiaspore development. The spores grew and reached a plateau of growth at 70 days post-infection. The median adiaspore diameter showed a plateau of around 40 μm. The characteristic three-layer cell-wall structure of adiaspores was observed in the lung at 70 days post-infection. We examined infection with a few spores, which revealed that adiaspores in the mouse lung progressed from intratracheal infection of at least 400 spores. Moreover, we developed adiaspores in vitro by culture in fetal bovine serum. Although most spores broke, some large spores were intact. They reached about 50 μm diameter. Thick cell walls and dense granules were found as common points between in vitro adiaspores and in vivo adiaspores. These models are expected to be useful for additional investigations of E. crescens adiaspores and adiaspiromycosis.

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Dimorphism in Chrysosporium parvum

Hejtmánek

1985

Fungal Dimorphism

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Scanning electron microscopy of experimental adiaspiromycosis

Abstract: Adiaspiromycotic granulomas of mice experimentally inoculated with fungus Emmonsia crescens Emmons et Jellison 1960 were examined by scanning electron microscopy. There morphology, surface structure, and germinating adiaspores isolated from granulomas are described.

Cited by 4 publications

References 3 publications

Adiaspiromycosis in Striped Skunks in Alberta, Canada

Adiaspiromycosis in Striped Skunks in Alberta, Canada

Adiaspore development and morphological characteristics in a mouse adiaspiromycosis model

Dimorphism in Chrysosporium parvum

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