Scanning Electron Microscopy of Endophytic Actinomycete Isolate against Fusarium oxysporum for Various Growth Parameters on Musk Melon

Kamboj, Priyanka; Gangwar, Madhurama; Singh, Narinder

doi:10.20546/ijcmas.2017.611.054

Cited by 4 publications

(2 citation statements)

References 11 publications

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“…Spores were flat and distorted as cytoplasmic structures were washed out from the cells. Our findings are consistent with several studies conducted by other researchers ( Kamboj et al., 2017 ; Chen et al., 2018 ). A study by Tang-um and Niamsup (2012) reported that Fusarium oxysporum f. sp.…”

Section: Discussionsupporting

confidence: 94%

Phylogenetic affiliation of endophytic actinobacteria associated with selected orchid species and their role in growth promotion and suppression of phytopathogens

2022

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Endophytic actinobacteria aid in plant development and disease resistance by boosting nutrient uptake or producing secondary metabolites. For the first time, we investigated the culturable endophytic actinobacteria associated with ten epiphytic orchid species of Assam, India. 51 morphologically distinct actinobacteria were recovered from surface sterilized roots and leaves of orchids and characterized based on different PGP and antifungal traits. According to the 16S rRNA gene sequence, these isolates were divided into six families and eight genera, where Streptomyces was most abundant (n=29, 56.86%), followed by Actinomadura, Nocardia, Nocardiopsis, Nocardioides, Pseudonocardia, Microbacterium, and Mycolicibacterium. Regarding PGP characteristics, 25 (49.01%) isolates demonstrated phosphate solubilization in the range of 61.1±4.4 - 289.7±11.9 µg/ml, whereas 27 (52.94%) isolates biosynthesized IAA in the range of 4.0 ± 0.08 - 43.8 ± 0.2 µg/ml, and 35 (68.62%) isolates generated ammonia in the range of 0.9 ± 0.1 - 5.9 ± 0.2 µmol/ml. These isolates also produced extracellular enzymes, viz. protease (43.13%), cellulase (23.52%), pectinase (21.56%), ACC deaminase (27.45%), and chitinase (37.25%). Out of 51 isolates, 27 (52.94%) showed antagonism against at least one test phytopathogen. In molecular screening, most isolates with antifungal and chitinase producing traits revealed the presence of 18 family chitinase genes. Two actinobacterial endophytes, Streptomyces sp. VCLA3 and Streptomyces sp. RVRA7 were ranked as the best strains based on PGP and antifungal activity on bonitur scale. GC-MS examination of ethyl acetate extract of these potent strains displayed antimicrobial compound phenol, 2,4-bis-(1,1-dimethylethyl) as the major metabolite along with other antifungal and plant growth beneficial bioactive chemicals. SEM analysis of fungal pathogen F. oxysporum (MTCC 4633) affected by Streptomyces sp. VCLA3 revealed significant destruction in the spore structure. An in vivo plant growth promotion experiment with VCLA3 and RVRA7 on chili plants exhibited statistically significant (p<0.05) improvements in all of the evaluated vegetative parameters compared to the control. Our research thus gives insight into the diversity, composition, and functional significance of endophytic actinobacteria associated with orchids. This research demonstrates that isolates with multiple plant development and broad-spectrum antifungal properties are beneficial for plant growth. They may provide a viable alternative to chemical fertilizers and pesticides and a sustainable solution for chemical inputs in agriculture.

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Section: Discussionsupporting

confidence: 94%

Phylogenetic affiliation of endophytic actinobacteria associated with selected orchid species and their role in growth promotion and suppression of phytopathogens

2022

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“…PRY2RB2, an endophyte of the medicinal plant Pseudowintera colorata (Horopito), a native of New Zealand, have shown inhibitory activity against various phytopathogens: Neofusicoccum luteum ICMP 16678, N. parvum MM562, Ilyonectria liriodendri WPA1C and Neonectria ditissima ICMP 14417 (Purushotham et al, 2018 ). Endophytic actinobacterium, strain AR3, isolated from Emblica officinalis , inhibited the growth of Fusarium oxysporum (Kamboj et al, 2017 ). Another endophyte, Streptomyces sp.…”

Section: Opportunities For Application Of Endophytic Actinobacteria Imentioning

confidence: 99%

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

2018

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Actinobacteria are wide spread in nature and represent the largest taxonomic group within the domain Bacteria. They are abundant in soil and have been extensively explored for their therapeutic applications. This versatile group of bacteria has adapted to diverse ecological habitats, which has drawn considerable attention of the scientific community in recent times as it has opened up new possibilities for novel metabolites that may help in solving some of the most challenging problems of the day, for example, novel drugs for drug-resistant human pathogens, affordable means to maintain ecological balance in various habitats, and alternative practices for sustainable agriculture. Traditionally, free dwelling soil actinobacteria have been the subject of intensive research. Of late, symbiotic actinobacteria residing as endophytes within the plant tissues have generated immense interest as potential source of novel compounds, which may find applications in medicine, agriculture, and environment. In the light of these possibilities, this review focuses on the diversity of endophytic actinobacteria isolated from the plants of extreme habitats and specific ecological niches. Furthermore, an attempt has been made to assign chemical class to the compounds obtained from endophytic actinobacteria. Potential therapeutic applications of these compounds and the utility of endophytic actinobacteria in agriculture and environment are discussed.

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Endophytic actinomycetes in bioactive compounds production and plant defense system

Aamir

Krishna

Zehra

et al. 2020

Microbial Endophytes

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Scanning Electron Microscopy of Endophytic Actinomycete Isolate against Fusarium oxysporum for Various Growth Parameters on Musk Melon

Cited by 4 publications

References 11 publications

Phylogenetic affiliation of endophytic actinobacteria associated with selected orchid species and their role in growth promotion and suppression of phytopathogens

Phylogenetic affiliation of endophytic actinobacteria associated with selected orchid species and their role in growth promotion and suppression of phytopathogens

Diversity and Applications of Endophytic Actinobacteria of Plants in Special and Other Ecological Niches

Endophytic actinomycetes in bioactive compounds production and plant defense system

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