Five-week-old male Wistar rats were used to study the effect of dietary therapy with ipriflavone combined with 1␣-OH-D 3. Ultrastructural alterations in the metaphysis of debilitated tibia were observed in the growing rats. I. Light microscopy findings In the low-calcium diet • standard diet with supplementary ipriflavone and 1␣-OH-D 3 group, calcification of the chondral matrix and ossification were active, and the tibia grew normally as in the control group. II. Scanning electron microscopy findings In the low-calcium diet • standard diet with supplementary ipriflavone and 1␣-OH-D 3 group, dense calcospherites, distinct chondral lacunae, regularly running collagen fibers, and distinct border lines were noted. III. Transmission electron microscopy findings In the low-calcium diet • standard diet with supplementary ipriflavone and 1␣-OH-D 3 group we found that the osteoblasts were active, the ruffled border of osteoclast was decrease, indicated this osteoclast is inactive. In conclusion, insufficient calcium intake during the developmental period resulted in debilitated(3etaphysis tibia, whereas dietary therapy using combined ipriflavone and 1␣-OH-D 3 promoted recovery. patients become refractory after several days of administration, which is known as the calcitonin escape phenomenon, therefore, it is not considered a suitable therapy for osteoporosis 3). Recently, research about the effect of ipriflavone (IF), which comes from beans and is an inducer of isoflavone, on bone has been focused on. Its structure is similar to estrogen, and it has been used as an inhibitor of bone resorption, though it is considered a non-hormonal drug for osteoporosis. It also has been demonstrated to have antiallergic, anti-inflammatory, and analgesic effects 2). Further, IF has also been reported to inhibit the activity of osteoclasts and promote that of osteoblasts 2,4,5). While other studies have focused on the effect of a combination therapy with IF and other substances 6,7). Bone mass reaches its peak in humans at about