Scanning Electron Microscopy of Cartilage

Boyde, A.; Jones, S. J.

doi:10.1016/b978-0-12-319501-2.50011-x

Cited by 22 publications

(40 citation statements)

References 69 publications

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“…Calcified cartilage was occasionally present on the epiphyseal side of growth plates in normal rats [1]. Under hypervitaminosis A, we found that high metachromatic and hypercalcified cartilage areae in the resting cell zone locally extended in the growth plates on the way from the epiphyseal side or throughout the plates longitudinally, but the hypercalcified cartilage contained higher S content than the non-calcified cartilage which was probably eosinophilic.…”

Section: Discussionmentioning

confidence: 78%

Local Disappearance of Epiphyseal Growth Plates in Rats with Hypervitaminosis A.

Kodaka

Takaki

Soeta

et al. 1998

The Journal of Veterinary Medical Science

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ABSTRACT. Epiphyseal growth plates of proximal tibiae in rats with high doses of vitamin A (V-A) were observed. Of 4 groups, each consisting of 5 rats, three groups were given V-A at doses, IU/100 g by body weight/day, of 50,000, 100,000, and 150,000, respectively. The other group rats were given no V-A (control). Rats were administered V-A for the 5 days from 4 weeks after birth and sacrificed at 12 weeks after birth. Three rats of the 150,000 IU group died during the period of observation. The decalcified sections were stained with hematoxylin-eosin or toluidine blue. In the ground sections, microradiography, backscattered electron imaging, and energy-dispersive Xray microanalysis were performed. These observations suggest that the local disappearance of epiphyseal growth plates under high doses of V-A goes in the order of the increased doses through the process of (1) calcified cartilage areae appearing in the resting cell zone, (2) some of the calcified areae extending in the growth plate towards the diaphysial side, (3) bone tissue replacing the calcified areae, and (4) the local disappearing of the growth plate. Such a local disappearance may be formed in the stressed proximal regions of tibiae. -KEY WORDS: calcified cartilage, epiphyseal growth plate, hypervitaminosis A, local disappearance, sulfur content.

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Section: Discussionmentioning

confidence: 78%

Local Disappearance of Epiphyseal Growth Plates in Rats with Hypervitaminosis A.

Kodaka

Takaki

Soeta

et al. 1998

The Journal of Veterinary Medical Science

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“…Mineralisation within rapidly growing growth plate cartilages as well as in other rapid cartilage growth and mineralisation sites is comparable between sites and species and has been reviewed elsewhere (Boyde and Jones, 1983a). The slow creeping mineralisation that occurs in articular cartilage and some fibrocartilage is collagen-based and gives a comparatively smooth front with prominences at sites of pericellular mineralisation Jones and Boyde, 1982).…”

Section: Initial Stages Of Mineralisationmentioning

confidence: 94%

Scanning electron microscopy of bone: Instrument, specimen, and issues

Boyde

Jones

1996

Microsc. Res. Tech.

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102

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“…Although the tidemark has been described as uncalcified (Stockwell, 1979), this should be taken to mean that it is not fully calcified, as pointed out by Fawns & Landells (1953), who introduced the term tidemark, and is implied by the alternative widely-used term, tidemark mineralizing-front (Boyde & Jones, 1983). This will be referred to again later because it is related to the question of what exactly is left behind on the bone surface when the uncalcified cartilage is destroyed by taphonomic processes or during investigative procedures.…”

Section: Calcified Cartilage As the Bare-bone Surfacementioning

confidence: 97%

“…According to Boyde & Jones (1983), who have done much to explore this layer, no way has been found to separate the two because the bone was originally deposited on the resorbed surface of the calcifying cartilage. According to Gray's Anatomy (Williams, 1995), the interface shows reciprocal fine ridges and grooves which interdigitate and so resist shearing stresses brought about by postural changes and muscle action.…”

Section: Calcified Cartilage As the Bare-bone Surfacementioning

confidence: 97%

Microscopic configurations on the bare‐bone surfaces of mammalian synovial joints

Miles

2001

Intl J of Osteoarchaeology

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The smoothness characteristic of synovial joint surfaces of bare bones is shown to be an illusion; low-power microscopy of young adult human bones from interments revealed, on the surfaces of a variety of synovial joints, a system of basically hemispherical elevations, often united as short chains or groups. This system was also found on joints of a variety of species of six mammalian orders. Under the higher magnification of scanning electron microscopy (SEM), many elevations had a pit at their summits. The bare-bone surfaces of synovial joints have a thin covering of mineralized cartilage, including its mineralizing-front, which survives taphonomic processes, as well as the preparative procedures used in the study of articular surfaces. In its formative phase, the front has the chondrocyte -columnar structure of cartilage. It is postulated here that the newly-discovered elevations arise when cartilage formation is ceasing, or becoming dormant, and that each column-unit produces a globular mineralized mass, often with a pit which had accommodated a chondrocyte. These masses may incorporate the fibre systems of the unmineralized cartilage and aid in its attachment to the bony surface.

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Scanning Electron Microscopy of Cartilage

Cited by 22 publications

References 69 publications

Local Disappearance of Epiphyseal Growth Plates in Rats with Hypervitaminosis A.

Local Disappearance of Epiphyseal Growth Plates in Rats with Hypervitaminosis A.

Scanning electron microscopy of bone: Instrument, specimen, and issues

Microscopic configurations on the bare‐bone surfaces of mammalian synovial joints

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