Scanning electron microscopy of aminonucleoside nephrosis

Carroll, Nanette; Crock, G. W.; Funder, C. C.; Green, C. R.; Ham, Kathryn N.; Tange, J. D.

doi:10.1002/path.1711110106

Cited by 10 publications

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References 11 publications

(14 reference statements)

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“…1) : first, there is evidence of increased heterophagy in that increased numbers of endocytic invaginations are seen along the plasma membrane, particularly those segments facing the basement membrane at the base of the foot processes, and increased numbers of phagosomes and protein absorption droplets (lysosomes) are seen within the cytoplasm. Second, the normal epithelial foot process organization is partially lost, and the slits are (22,23), that this change is due to withdrawal of the foot processes and their replacement by broad sheets of cytoplasm plastered along the outer aspect of the basement membrane, and that this process is continuous and progressive in acute nephrosis. Third, some of the remaining slits are altered to form occluding junctions, i.e ., the outer leaflets of the plasma membrane of two epithelial cells sometimes appear fused in sectioned material (Fig .…”

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The permeability of glomerular capillaries of aminonuceoside nephrotic rats to graded dextrans.

Caulfield¹,

Farquhar²

1975

The Journal of Experimental Medicine

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We have recently used graded dextrans as tracers to investigate glomerular permeability in normal rats (1) in order to determine which structure in the glomerular capillary wall, the basement membrane or the epithelial slits, represents the primary filtration barrier to macromolecules . Three dextran fractions of specified mol wt (40,000, 62,000, and 125,000) were prepared which bracket the size of albumin, the molecule effectively retained by the normal glomerulus. When injected intravenously into rats, the dextran fractions behaved as predicted in that they were extensively filtered, filtered in small amounts, and extensively retained, respectively . In all three cases dextran particles were found initially in high concentration in the capillary lumen and in the subendothelial portions of the basement membrane but there was a sharp drop in their concentration at this level, i.e., between the inner looser portions of the basement membrane and its outer more compact portions . With the two largest fractions accumulation of particles occurred against the basement membrane in mesangial regions with time, but no accumulation was ever seen with any of the fractions in the epithelial slits or against the slit membranes . Therefore, we concluded that the results obtained with dextrans, like those obtained previously using ferritin as a tracer (2, 3), identify the glomerular basement membrane as the main barrier to the passage of macromolecules of the size of albumin in the normal glomerulus.In order to extend these observations we have carried out similar studies on animals with experimental nephrosis (induced with an aminonucleoside of puromycin) . Nephrotic animals represent an interesting situation in which to study glomerular permeability since they show albuminuria due to increased leakage of albumin by the glomerulus, accompanied by profound changes in the glomerular capillary wall (cf. 4) : the usual epithelial foot process organization is lost with a concomitant reduction in the number of epithelial filtration slits, the epithelial cells become filled with protein absorption droplets (i.e., phagosomes and lysosomes), and the basement membrane appears thinner and less compact in some regions.

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The permeability of glomerular capillaries of aminonuceoside nephrotic rats to graded dextrans.

Caulfield¹,

Farquhar²

1975

The Journal of Experimental Medicine

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Puromycin aminonucleoside nephropathy: Ultrastructure, glomerular polyanion, and cell surface markers

Rossmann

Bukovský

Matoušovic

et al. 1986

The Journal of Pathology

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Puromycin aminonucleoside nephropathy with heavy proteinuria and oedema was induced in rats by 10 consecutive daily subcutaneous injections of aminonucleoside (1.67 mg/100 g of body weight). The main ultrastructural lesions were vacuolation of podocytes and total fusion of foot processes with loss of colloidal iron-reactive polyanion layer on the epithelial surface adjacent to the basement membrane. On the other hand the outer surface of podocytes and intravacuolar granular substance stained with colloidal iron. In scanning electron microscopy of freeze-fractured tissue the swollen podocytes and the urinary spaces displayed granular and filamentous precipitates. Seven cell surface antigens were examined by indirect enzyme immunohistochemistry with a series of MRC OX monoclonal antibodies. Glomeruli of control rats exhibited rare isolated Ia- positive endocapillary cells, possibly monocytes; these elements were significantly reduced in puromycin aminonucleoside nephropathy but there was an increase in Ia- positive cells in the cortical interstitium. Control kidneys harboured scanty interstitial T lymphocytes. These latter, especially the T8- positive cytotoxic/suppressor subpopulation, were markedly augmented in puromycin aminonucleoside nephropathy. The expression of class I histocompatibility antigens and of differentiation antigens (Thy 1) was not altered by aminonucleoside.

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Effects of Puromycin Aminonucleoside on Ten Urinary Enzymes in Rats

Bomhard

Maruhn

1988

Archives of Toxicology

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Scanning electron microscopy of aminonucleoside nephrosis

Cited by 10 publications

References 11 publications

The permeability of glomerular capillaries of aminonuceoside nephrotic rats to graded dextrans.

The permeability of glomerular capillaries of aminonuceoside nephrotic rats to graded dextrans.

Puromycin aminonucleoside nephropathy: Ultrastructure, glomerular polyanion, and cell surface markers

Effects of Puromycin Aminonucleoside on Ten Urinary Enzymes in Rats

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