Scanning Electron Microscopy Approach for Evaluation of Hair Dyed with Lawsonia inermis Powder: in vitro Study

Bianchi, Serena; Bernardi, Sara; Continenza, Maria Adelaide; Vincenti, Eleonora; Antonouli, Sevastiani; Torge, Diana; Macchiarelli, Guido

doi:10.4067/s0717-95022020000100096

Cited by 9 publications

(3 citation statements)

References 19 publications

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“…While transcriptomic analysis has revealed profound changes in gene expression in embryos cultured under different oxygen concentrations [37], the morphologic effect of oxygen on embryos is not well known, and was first described by our group [5]. To this end, the current detailed ultrastructural analysis provides a deeper understanding of events occurring during development [28,[38][39][40][41], and allows us to establish a partial correlation between the observed phenotype and the transcriptomic assay, with clinical implications that are valuable in several fields [42][43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Belli

Rinaudo

Palmerini

et al. 2020

IJERPH

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Assisted Reproductive Technologies routinely utilize different culture media and oxygen (O2) concentrations to culture human embryos. Overall, embryos cultured under physiological O2 tension (5%) have improved development compared to embryos cultured under atmospheric O2 conditions (20%). The mechanisms responsible for this remain unclear. This study aimed to evaluate the effect of physiologic (5%) or atmospheric O2 (20%) tension on the microscopic ultrastructure of pre-implantation mouse embryos using Transmission Electron Microscopy (TEM). Embryos flushed out of the uterus after natural mating were used as the control. For use as the control, 2-cells, 4-cells, morulae, and blastocysts were flushed out of the uterus after natural fertilization. In vitro fertilization (IVF) was performed using potassium simplex optimized medium (KSOM) under different O2 tensions (5% and 20%) until the blastocyst stage. After collection, embryos were subjected to the standard preparative for light microscopy (LM) and TEM. We found that culture in vitro under 5% and 20% O2 results in an increase of vacuolated shaped mitochondria, cytoplasmic vacuolization and presence of multi-vesicular bodies at every embryonic stage. In addition, blastocysts generated by IVF under 5% and 20% O2 showed a lower content of heterochromatin, an interruption of the trophectodermal and inner cell mass cell membranes, an increased density of residual bodies, and high levels of glycogen granules in the cytoplasm. In conclusion, this study suggests that in vitro culture, particularly under atmospheric O2 tension, causes stage-specific changes in preimplantation embryo ultrastructure. In addition, atmospheric (20%) O2 is associated with increased alterations in embryonic ultrastructure; these changes may explain the reduced embryonic development of embryos cultured with 20% O2.

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Section: Discussionmentioning

confidence: 99%

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Belli

Rinaudo

Palmerini

et al. 2020

IJERPH

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“…Pre-and post-clean-up replicas were dried with compressed air, mounted on an aluminum platform, and analysed under controlled pressure with a scanning electron microscope (SEM), as previously described [10][11][12][13][14] (XL 30 CP, Philips, Eindhoven, The Netherlands). SEM observations and comparisons of the pre-treatment images and the post-treatment images allowed us to distinguish the residual adhesive (Figures 1-3).…”

Section: Scanning Electron Microscope (Sem) Analysismentioning

confidence: 99%

Debonding and Clean-Up in Orthodontics: Evaluation of Different Techniques and Micro-Morphological Aspects of the Enamel Surface

et al. 2020

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There is currently no consensus on the best way to remove adhesive remnants from teeth following debonding. The main objective of this study is to evaluate and compare the effectiveness of four adhesive resin removal (clean-up) techniques, performed with or without the use of an operative microscope. Forty human teeth were duplicated using an epoxy resin for impregnation. Brackets were bonded to teeth and debonded from teeth. Then, the samples were randomly divided into two equal groups—the naked eye group and the magnification group—and further subdivided into four equal subgroups, in order to compare the different techniques used for the clean-up. Each subgroup was formed of five natural teeth with the respective pre- and post-bonding replicas. Macro- and micro-analysis by means of a stereomicroscope and scanning electron microscopy evaluated, qualitatively and quantitatively, the adhesive remnant index and the damage index of the enamel. Overall, the magnification improved the removal of resins compared to the naked eye (p < 0.001), and the use of magnification constantly reduced resin residual and surface damage. Enamel damage and adhesive residual from the clean-up procedures represent an ascertained risk in orthodontics. The use of a magnification system improves the quality of debonding and clean-up techniques in a significant way.

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“…Human hair is sensitive to aging and environmental dynamics, specifically when induced by genetic and epigenetic factors. In this perspective, a primary role is reserved to heavy metal compounds [23]. Therefore, an analysis using hair samples was conducted to determine lead levels which can be an alternative way to measure biological indicators to observe a history of lead exposure in children due to its accessibility and practicality.…”

Section: Introductionmentioning

confidence: 99%

Hair Lead Levels as an Alternative Indicator to Measure Lead Level in Children Body

Setyoko¹,

Hairat²,

Rachmat³

et al. 2023

ujph

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Informal recycling of lead-acid batteries produces uncontrollable pollution with a negative impact on health, particularly for children living near recycling areas. This study aimed to determine the correlation between blood lead levels (BLL), the gold standard to measure lead levels in human body, and hair head levels (PbH) as an alternative indicator. The study was conducted in 2014 as part of environmental pollution research. The research was focused on informal recycling of lead-acid batteries with cross-sectional design involving children (aged 7 to 13 years) who lived close to lead-acid battery recycling activities in Depok City, Indonesia. Blood lead level was measured using anodic stripping voltammetry method and hair lead level using atomic absorption spectrometry. The measurements resulted in an average BLL of 8.22 µg/dL and PbH of 8.23 µg/g. The correlation between blood level and hair level is strong with coefficient correlation of 0.543 (p<0.05). The linear equation displays a positive pattern (y=1,573x-4,705), suggesting that when BLL increases, PbH increases as well. Hair lead levels can be used as an alternative indicator to measure lead levels in children.

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Scanning Electron Microscopy Approach for Evaluation of Hair Dyed with Lawsonia inermis Powder: in vitro Study

Cited by 9 publications

References 19 publications

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Pre-Implantation Mouse Embryos Cultured In Vitro under Different Oxygen Concentrations Show Altered Ultrastructures

Debonding and Clean-Up in Orthodontics: Evaluation of Different Techniques and Micro-Morphological Aspects of the Enamel Surface

Hair Lead Levels as an Alternative Indicator to Measure Lead Level in Children Body

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