Scanning electron microscopy applied to seed‐borne fungi examination

Alves, Marcelo de Carvalho; Pozza, Edson Ampélio

doi:10.1002/jemt.20695

Cited by 20 publications

(13 citation statements)

References 11 publications

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“…The utilization of SEM has brought pioneering trend for detailed examination of the microscopic organisms. This technique has been used to study seed born fungi (Alves & Pozza, ) and ornithogalum species (Bednorz & Czarna, ). Little work with no emphasis on taxonomic implication is available on the SEM of legume seeds (Brisson & Peterson, ; Gandhi, Albert, & Pandya, ).…”

Section: Introductionmentioning

confidence: 99%

Scanning electron microscopy leads to identification of novel nonedible oil seeds as energy crops

Munir

Ahmad

Waseem

et al. 2019

Microscopy Res & Technique

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Currently, exploration of alternative energy resources is hotly debated among the scientific community owing to rising energy crises and environmental issues. Biodiesel, as renewable energy source proves to be a better option and substitute to petro diesel. In this regard, nonedible seeds could be a better feedstock for synthesizing biodiesel due to their cost effectiveness and environmental friendly attributes. The present study, therefore, deals with the exploration and identification of micromorphologic features among eight novel nonedible oil yielding seeds via scanning electron microscopy (SEM) as potential feedstock for biodiesel industry. Light microscopic studies revealed that seeds size vary from 0.1-2.9 cm in length to 0.1-3 cm in width. Moreover, a great variation in seed color from black, green, and different shades of brown was also observed. Seeds ultrastructure examination by SEM exhibit great variation in seed shape, size, color, sculpturing and periclinal wall shape and arrangement and so on. All the understudy seeds vary from rounded, irregular, subspherical, ellipsoidal, reniform, flattened, polygonal, ovate, pyriform, oblong, and globose shape. Seeds wall structure exhibits great variation from entire, angular, straight, irregular, polygonal, smooth, and elongated. The periclinal wall pattern exhibits variation from flat to slightly concave-convex with straight, angular, undulate, or dentate seeds margin. Among the studied species only Argemone ochroleuca Linn. (Papaveraceae) possess micropylar peak, ridged raphe, and basal helium. The obtained results from the present study would therefore, suggest that SEM could be a useful tool in refreshing the veiled micromorphological features among different oil yielding seeds which in turn helps the researchers for their correct identification, exploration, authentication, and seeds classification in future. K E Y W O R D S energy crises, identification, nonedible oil yielding seeds, scanning electron microscopy, seed micromorphology 1 | INTRODUCTIONEnergy is vital in our lives to streamline transportation, industrial, and power generation sectors. Globally, energy demand has been satisfied by the fossil fuel reservoirs and its urgency seems to increase steadily due to significant competences provided by the unique and renewable technologies followed by the implementation of meticulous energy plans and environmental regulations (Milano et al., 2018). This scenario had raised international awareness of energy crises due to the dramatic shift in fuel prices along with the dwindling of fossil fuel

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Section: Introductionmentioning

confidence: 99%

Scanning electron microscopy leads to identification of novel nonedible oil seeds as energy crops

Munir

Ahmad

Waseem

et al. 2019

Microscopy Res & Technique

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show abstract

“…Seed coat external features such as sculpturing and texture are considered to be fairly variable and additional morphological attribute and hence prove the systematic importance among variety of species (Al‐Gohary & Mohamed, ; Belhadj et al, ). Seed bearing fungi has been examined using SEM, which brought revolution in the scope of microbiology (Alves & Pozza, ). The present study describes the macro‐ and micromorphological features like seed shape, size, color, surface cells shape, surface cells arrangement, periclinal wall level, and anticlinal wall level and pattern, as additional taxonomic characters of the seeds for nine species of Eucalyptus in Pakistan.…”

Section: Introductionmentioning

confidence: 99%

Micromorphological observation of seed coat of Eucalyptus species (Myrtaceae) using scanning electron microscopy technique

Luqman

Zafar

Ahmad

et al. 2018

Microscopy Res & Technique

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Seed shape, surface cells shape, arrangement, anticlinal wall pattern, and periclinal wall protuberances were recorded for nine species of Eucalyptus (Myrtaceae) using scanning electron microscopy to determine the importance of seed morphological characters as an additional tool for identification. Most of the seeds were found ovate and some seeds were elliptic and cuboid in shape. Almost reticulate regular seed surface patterns were observed. Four types of surface cells were examined; diamond, elliptic, oblong, and irregular. Majority of the seeds showed raised anticlinal wall level and diversity from wavy to puzzle in pattern. Periclinal wall may be glabrous or having protuberances that were rhombus and bullate in shape. Both macro-and micromorphological characters can provide basis for classification and delimitation of genus Eucalyptus. Research highlights• Seeds quantitative characters of nine Eucalyptus species as seed length, width, and weight.• Macromorphological characters of seeds including seed color, helium position, and seed shape.• Micromorphological characters include seed surface, periclinal wall, and anticlinal wall investigation under scanning electron microscope.• Ultra-seed sculpturing features as an additional tool in identification. K E Y W O R D S eucalyptus, micromorphology, Myrtaceae, seed, SEM

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“…Few observations of natural microscopic communities have been made on seeds, but this is possible with scanning electron microscopy [14]. It would be similarly possible to sample fur from the lined pouches of Kangaroo Rats for electron microscopy.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity, Recombination, and Divergence in Animal Associated Penicillium dipodomyis

Henk

Fisher

2011

PLoS ONE

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Penicillium dipodomyis is thought to be an exclusively asexual fungus associated with Kangaroo Rats, Dipodomys species, and is unique among Penicillium species in growing at 37°C but producing no known toxins. Lack of recombination within P. dipodomyis would result in limited adaptive flexibility but possibly enhance local adaptation and host selection via maintenance of favourable genotypes. Here, analysis of DNA sequence data from five protein-coding genes shows that recombination occurs within P. dipodomyis on a small spatial scale. Furthermore, detection of mating-type alleles supports outcrossing and a sexual cycle in P. dipodomyis. P. dipodomyis was a weaker competitor in in vitro assays with other Penicillium species found in association with Kanagaroo rats. Bayesian species level analysis suggests that the P. dipodomyis lineage diverged from closely related species also found in cheek pouches of Kangaroo Rats and their stored seeds about 11 million years ago, a similar divergence time as Dipodomys from its sister rodent taxa.

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Scanning electron microscopy applied to seed‐borne fungi examination

Cited by 20 publications

References 11 publications

Scanning electron microscopy leads to identification of novel nonedible oil seeds as energy crops

Scanning electron microscopy leads to identification of novel nonedible oil seeds as energy crops

Micromorphological observation of seed coat of Eucalyptus species (Myrtaceae) using scanning electron microscopy technique

Genetic Diversity, Recombination, and Divergence in Animal Associated Penicillium dipodomyis

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