Scanning electron microscopy—a powerful imaging technique for the clinician

Bazin, Dominique; Bouderlique, Elise; Daudon, Michel; Frochot, Vincent; Haymann, Jean‐Philippe; Letavernier, Emmanuel; Tielens, Frederik; Weil, Raphaël

doi:10.5802/crchim.101

Cited by 16 publications

(4 citation statements)

References 201 publications

(245 reference statements)

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“…In this context, SEM is a relevant tool to study BMCs morphology at the sub-micrometer scale [59][60][61] without any specific sample preparation except those already implemented in most anatomopathological laboratories and used for medical diagnosis [62,63].…”

Section: Sem: Sub-micrometer Scale Morphological Descriptionmentioning

confidence: 99%

Multiscale approach to provide a better physicochemical description of women breast microcalcifications

Petay¹,

Cherfan²,

Bouderlique³

et al. 2022

Comptes Rendus. Chimie

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Despite the incidence of breast cancer among women, mammography and anatomopathology investigations are still the gold standard method for preventive screening and diagnosis. Several criteria are used to diagnose precisely the severity of the pathology like the distribution and shape of breast microcalcifications (BMCs). However, the link between the different chemical phases of BMCs and the cancer stage remains unclear. As BMCs physicochemical speciation has the potential to help clinicians during their diagnosis, this study aims to propose a methodology using advanced spectroscopical analysis techniques to finely characterize BMCs and uncover the relationship between

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Section: Sem: Sub-micrometer Scale Morphological Descriptionmentioning

confidence: 99%

Multiscale approach to provide a better physicochemical description of women breast microcalcifications

Petay¹,

Cherfan²,

Bouderlique³

et al. 2022

Comptes Rendus. Chimie

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“…In the kidney for instance many chemical compounds without calcium have been identified (Figure 2). Field Emission Scanning Electron Microscopy (FE-SEM) offers the opportunity to observe them at the submicrometer scale [77][78][79][80].…”

Section: On the Definition Of Crystalline Pathologiesmentioning

confidence: 99%

“…Some of them are "classics" i.e. Raman and Infra-Red spectroscopies [136][137][138][139], Second Harmonic Generation (SHG), Scanning and Transmission Electron Microscopy (SEM and TEM) [80,[140][141][142], µX-ray fluorescence [143][144][145][146][147], µ X-ray diffraction [148][149][150][151][152][153] or Nuclear Magnetic Resonance (NMR) [154][155][156][157][158][159].…”

Section: Characterization By Physicochemical Techniquesmentioning

confidence: 99%

Foreword to microcrystalline pathologies: combining clinical activity and fundamental research at the nanoscale

Bazin¹,

Daudon²,

Frochot³

et al. 2022

Comptes Rendus. Chimie

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This contribution underlines the key role of physicochemical characterisation techniques in the area of medical research. The starting point centres on the Mid-InfraRed platform located at the Tenon hospital and dedicated to multidisciplinary functional investigations. In the last two decades, we have enhanced this platform by creating a network combining researchers from varied disciplines such as physicists, chemists, and clinicians. The resultant research dynamism is underscored by metrics such as 71 references in Pubmed and 129 in Web of Science, and the high impact of the journals in which we have published (New England Journal of Medicine, Kidney International, Chemical Review. . . ). It is of paramount importance to disseminate these physicochemical techniques among young doctors, and to establish collaborations with appropriate private companies.

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“…Kidney stone formation, also known as nephrolithiasis is the result of an imbalance between supersaturated solutes and crystallization inhibitors in urine. [1][2][3][4] More precisely, the low solubility of L-cystine (about 240 mg/L in urine) may explain its crystallization in the nephrons and formation of kidney stones observed in patients with cystinuria. Due to its very high water solubility (about 280 g/L), L-cysteine crystal may not form in body fluids or urine, but L-cystine and/or L-cysteine may accumulate within lysosome cells in patients suffering from a rare inherited disease named cystinosis which results from mutations of CTNS gene.…”

Section: Introductionmentioning

confidence: 99%

Crystalline structures of l-cysteine and l-cystine: a combined theoretical and experimental characterization

Hessou

Colombo

et al. 2022

Amino Acids

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It is assumed that genetic diseases affecting the metabolism of cysteine and the kidney function lead to two different kinds of pathologies, namely cystinuria and cystinosis whereby generate L-cystine crystals. Recently, the presence of L-cysteine crystal has been underlined in the case of cystinosis. Interestingly, it can be strikingly seen that cystine ([-S-CH2-CH-(NH2)-COOH]2) consists of two cysteine (C3H7NO2S) molecules connected by a disulfide (S-S) bond. Therefore, the study of cystine and cysteine is important for providing a better understanding of cystinuria and cystinosis. In this paper, we elucidate the discrepancy between L-cystine and L-cysteine by investigating the theoretical and experimental infrared spectra (IR), X-ray diffraction (XRD) as well as Raman spectra aiming to obtain a better characterization of abnormal deposits related to these two genetic pathologies.

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Scanning electron microscopy—a powerful imaging technique for the clinician

Cited by 16 publications

References 201 publications

Multiscale approach to provide a better physicochemical description of women breast microcalcifications

Multiscale approach to provide a better physicochemical description of women breast microcalcifications

Foreword to microcrystalline pathologies: combining clinical activity and fundamental research at the nanoscale

Crystalline structures of l-cysteine and l-cystine: a combined theoretical and experimental characterization

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