Scanning electron microscopy: a direct method of identifying pollen grains on moths (Noctuidae: Lepidoptera)

Turnock, W. J.; Chong, J.; Luit, B. van

doi:10.1139/z78-275

Cited by 16 publications

(14 citation statements)

References 6 publications

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“…Mikkola (1971) combed the Lepidopteran body over a glass slide and rolled the proboscis in euparal, then examined the slide with light microscopy (LM). Hendrix et al (1987) and Turnock et al (1978) also examined the exterior of adult Lepidoptera but used a combination of LM and scanning electron microscopy (SEM). Courtney et al (1982) shortened the external examination process by using only SEM and examined the head, proboscis, palps and body.…”

Section: Insect Migration and Foraging Resourcesmentioning

confidence: 99%

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The uses of pollen and its implication for Entomology

Jones¹,

Jones²

2001

Neotrop. Entomol.

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Os Usos do Pólen e suas Implicações na Entomologia RESUMO -Palinologia é o estudo dos grãos de pólen produzidos por plantas sementeiras (angiospermas e gimnospermas) e esporos (pteridófitas, briófitas, algas e fungos). O pólen representa a flora terrestre e pode ser útil em muitas disciplinas: é único, não deteriora facilmente e é um marcador natural. O pólen pode ser usado para determinar os mecanismos de polinização, os recursos de forrageamento, as rotas de migração e locais fontes de insetos e outros polinizadores. O pólen fornece meios para análises paleoambientais de rochas terrestres sendo de interesse de geólogos. Também auxilia a explicar as correlações de ambientes terrestres com as sucessões marítimas e é usado para determinar trocas ecológicas e ambientais. Recentemente, a palinologia tem sido utilizada nos meios forenses. Esse artigo introduz a idéia do uso do pólen em várias disciplinas e, em particular, na entomologia. PALAVRAS-CHAVE: Pólen, entomopalinologia, forense, aerobiologia.ABSTRACT -Palynology is the study of pollen grains produced by seed plants (angiosperms and gymnosperms) and spores (pteridophytes, bryophytes, algae and fungi). It represents the land flora and can be use in many different disciplines. Pollen is distinctive, does not easily decay and is a natural marker. Pollen can be used to determine pollination mechanisms, foraging resources, migration routes and source zones of insects and other pollinators. It provides a means for paleoenvironmental analyses of terrestrial rocks and is of interest to geologists. Pollen also aids in the correlation of terrestrial with marine successions and is used to determine environmental and ecological changes. Recently, palynology is used in forensics. This manuscript introduces the ideas of using pollen in a variety of disciplines, in particular in entomology.

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Section: Insect Migration and Foraging Resourcesmentioning

confidence: 99%

“…Although Turnock et al (1978) found pollen within the proboscis tube, they did not examine the crop (insect organ that is the receptacle for food) or other parts of the digestive system for pollen. The idea that pollen is drawn into the digestive system at the time of feeding has not been examined.…”

Section: Insect Migration and Foraging Resourcesmentioning

confidence: 99%

The uses of pollen and its implication for Entomology

Jones¹,

Jones²

2001

Neotrop. Entomol.

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“…Methods for preparing specimens for SEM were similar to those described by Turnock et al (1971). Moths were relaxed in a humid environment for at least 2 h before the proboscis and (for some moths) the antennae and legs were removed using fine forceps.…”

Section: Scanning Electron Microscopymentioning

confidence: 99%

“…because of the numbers which need to be marked to compensate for losses between marking and capture, and because such markers may affect viability. Moth-borne pollen is a natural marker which avoids these difficulties (Mikkola 1971, Turnock et al 1971, Hendrix et al 1987. Helicoverpa spp.…”

Section: Introductionmentioning

confidence: 99%

“…Pollen is usually mor-phologically distinct at the family level, and sometimes at the generic or specific levels. Scanning electron microscopy is a powerful technique for revealing these distinctions (Turnock et al 1971, Iwanami et al 1988), These properties of pollen make it possible to match the composition of pollen carried on suspected immigrants with that of the flora in potential source areas, Hendrix et al (1987) used pollen to study migration oi Helicoverpa zea between southern Texas and Arkansas, a distance of over 750 km. Pollen of Pithecellobium sp, was found on 11 moths, and that of Calliandra sp, on 8 moths, from a total of 2721 collected in Arkansas, The nearest records of these plants are in southern Texas, In the present study, moth-borne pollen oi Helicoverpa spp, collected in September 1989 in western Queensland is compared with that of moths collected from eastern Australia in September 1989 and 1990,…”

Section: Introductionmentioning

confidence: 99%

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Pollen as a marker for migration of Helicoverpa armigera and H. punctigera (Lepidoptera: Noctuidae) from western Queensland

Gregg

1993

Australian Journal of Ecology

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Pollen carried on the probosces of Helicoverpa punctigera (Wallengren) and H. armtgera (Hiibner) trapped in western Queensland and in cropping areas of eastern Australia in September 1989 and 1990 was identified by scanning electron microscopy. Ninety-five per cent of moths carried pollen. A total of 19 'morphological pollen species', representing 14 plant families, was found. Up to six pollen species were found on individual moths, and 61% carried more than one. Pollen from plants unsuitable for larval survival was common. Pollen loads generally reflected the abundance of locally flowering plants, but there were exceptions which suggested migration. Pollen oi Ptilotus (Amaranthaceae), Velleia (Goodeniaceae) and Eremophila (Myoporaceae), and the Asteraceae (Tubuliflorae) were found on moths trapped in the east. These plants either did not occur in the areas where the moths were caught, or did not flower there at the time the moths were caught. However, they were abundant in possible source areas such as western Queensland. Among moths caught in eastern regions, 30% of H. punctigera and 18% of H. armigera carried pollen from such plants. The value and limitations of moth-borne pollen as a marker for migration are discussed.

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