Scanning Electron Microscopy

Johari, O.; Samudra, A. V.

doi:10.1007/978-1-4613-4490-2_6

Cited by 4 publications

(2 citation statements)

References 7 publications

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“…In some cases among the mitochondria of a usual appearance there appear also small amounts of mitochondria with a strikingly light matrix and slightly damaged cristae. They are probably disintegrating mitochondria as was also stated by Kaplan and Meyer (1959), Meachim et al (1965), Weiss and Mirrow (1972), Ghadially et al (1970), Mitchell and Shepard (1970), Dearden et al (1974), Gritzka et al (1974), Refior (1974), Rother and Redel (1975), Hanaoka (1976), Podrushniak and Cerkasov (1976), Silberger et al (1976), and others especially on the basis of investigations of these organelles in ageing cartilage and initial forms of arthroses. Ri b 0 so m es in the cytoplasm of chondrocytes of the superficial layer are mostly bound to membrane of the granular endoplasmic reticulum.…”

Section: B) Cytoplasmmentioning

confidence: 59%

Submicroscopic Structure of the Human Joint Cartilage

Horký¹

1980

Acta Vet. Brno

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H 0 r k yD.: Submicroscopic Structure of the Human Joint Cartilage. Acta vet. Brno, 49, 1980: 145-176.Using a transmission and scanning electron microscope the joint cartilage was studied in 43 persons of the age from 5 to 75 years. As far as possible the present study gives a finished survey of the ultrastructure of chondrocytes of the superficial, middle and deep layers of the articular cartilage, arrangement of the intercellular substance; presented are basic data on the formation of the surface of the cartilage with regard to the age of the object. Special attention is given to cartilage intercellular substance which -from the point of view of electron microscopy -has been studied only little. The components of intercellular substance are arranged in such a way that we can distinguish the specialized areas near the chondrocytes as the pericellular matrix on the one hand, and the other intercellular substance, the intercellular matrix, on the other. The surface of the cartilage is covered with a specialized layer designated as lamina splendens. Results of the study proper are confronted with published data not only with regard to human joint cartilage but data on these structures have been studied also in lower mammals as much knowledge holds generally true for this type of tissue. The study summarizes the known facts about joint cartilage and provides a necessary basis for judging the degrees of changes occurring during some of their injuries. Ultrastructure, hip joint cartilage, chondrocytes, intercellular matrix, SEM.A number of authors dealt with the description of submicroscopic structure of joint cartilage; principal studies dealing with human cartilage were published by Zelander (1959), Godman et al. (1960), Fawcett (1966a), Meachim (1967), Stockwell (1967a), Silberger (1968, Weiss et al. (1968). Brower and Hsu (1969), Roy (1969), Meachim (1969), Meachim and Roy (1969), Hirohata and Morimoto (1971), Stockwell andMeachim (1973), Serafini-Fracassini and Smith (1974), and others. The present study gives the most possible finished survey about the ultrastructure of both the chondrocytes of the joint cartilage and the intercellular substance; given are also basic data on the formation of its surface in dependence on the age of the object. Results of our study are confronted with published data not only with regard to human joint cartilage but also to data on lower mammals as much knowledge holds generally true for this type of tissue. This fact has been proved in studies published by Barnett et al.

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Section: B) Cytoplasmmentioning

confidence: 59%

Submicroscopic Structure of the Human Joint Cartilage

Horký¹

1980

Acta Vet. Brno

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“…Scan rate or electron flux dependent contrast has been well understood for some time. 54,57,58 Charge injected into dielectric materials usually does not stay trapped permanently. Rather, it leaks away at a characteristic rate that depends, among other things, on the local stored charge density.…”

Section: Charge Contrast Imagingmentioning

confidence: 99%

Imaging and analysis in materials science by low vacuum scanning electron microscopy

Thiel¹

2004

International Materials Reviews

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Low vacuum scanning electron microscopes (SEMs) enable stable imaging of non-conductive samples by allowing a small pressure of gas in the specimen chamber. However, the gaseous ions that electrically stabilise the specimens can also give rise to potentially useful new contrast mechanisms. The charge stabilisation process in these instruments is summarised and recent efforts to understand the new complex, dynamic contrast mechanisms are reviewed. Several significant low vacuum and environmental SEM applications papers are critically considered, and the results evaluated in light of the imaging theory presented. Although the applications have been chosen to represent a wide range of topics of interest to materials scientists, a slight emphasis has been placed on applications relevant to the electronics industry. In particular, it should be noted that many of the dynamic contrast effects have characteristic time constants that are comparable to experimental parameters such as scan rate. The potential for using these effects to map electronic inhomogeneities and defects is discussed.

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Surface Characterization in Polymer/Metal Adhesion

Filbey

Wightman

1991

Adhesive Bonding

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Scanning Electron Microscopy

Cited by 4 publications

References 7 publications

Submicroscopic Structure of the Human Joint Cartilage

Submicroscopic Structure of the Human Joint Cartilage

Imaging and analysis in materials science by low vacuum scanning electron microscopy

Surface Characterization in Polymer/Metal Adhesion

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