Scanning Electron Microscopic Studies on Blood Vessels in Cancer of the Larynx

Miodoński, A; Kuś, Jan; Olszewski, E; Tyrankiewicz, Ryszard

doi:10.1001/archotol.1980.00790300009003

Cited by 28 publications

(9 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar structures were described as ''glomerular loops'' in human squamous cell carcinoma of the larynx (Miodon˜ski et al 1980), in human hemangiomatosis (Arashiro et al 1995), and in rat mammary tissue (Kaidoh et al 1991).…”

Section: Discussionmentioning

confidence: 62%

Angioarchitecture of squamous cell carcinoma from hamster buccal pouch: a scanning electron microscopy study

et al. 2009

View full text Add to dashboard Cite

Tumoral angiogenesis has been widely studied by histochemical analysis but little has been done regarding morphology of these new vessels. The objective of this study was to perform a qualitative analysis of the angiogenic response to chemical induction with dimethylbenzanthracene (DMBA) and carbamide peroxide of squamous cell carcinoma in pouches of Syrian hamsters after different periods of treatment. Twenty-four Syrian golden hamsters, divided into three groups of eight animals each, had their right jugal pouches treated with a 5% DMBA solution three times a week and a 10% carbamide peroxide two times a week for 55, 70 and 90 days. The left pouch was considered the control. After tumor induction, five animals in each group had their pouches prepared for analysis under scanning electron microscopy and three animals for analysis under light microscopy. The control pouches showed a vascular system composed by few main vessels running parallel to the longest axis of the pouch with some branches. In the pouches submitted to tumor induction, a well-differentiated squamous cell carcinoma was present since 55 days induction in all samples. The new vascular system showed the presence of many tortuous vessels and the majority of them were veins and capillaries. Terminal loops were extremely sinuous adopting a glomerular or corkscrew shape. These tumor vessels are different from normal vessels, presenting irregular diameters, outpouchings and constrictions. Angiogenesis of sprouting and intussusceptive kind could be identified in the tumor pouches, and they were more frequent as the tumor developed.

show abstract

Section: Discussionmentioning

confidence: 62%

Angioarchitecture of squamous cell carcinoma from hamster buccal pouch: a scanning electron microscopy study

et al. 2009

View full text Add to dashboard Cite

show abstract

“…One of the most impressive findings was the clear diffusion of angiogenic buttons in the nail root, probably as a result of the nail regenerating process already reported in pathological conditions (Miodonski et al. 1980; Tano et al.…”

Section: Discussionmentioning

confidence: 68%

Microvascularization of the human digit as studied by corrosion casting

et al. 2004

View full text Add to dashboard Cite

The aim of this study was to describe microcirculation in the human digit, focusing on the vascular patterns of its cutaneous and subcutaneous areas. We injected a functional supranumerary human thumb (Wassel type IV) with a low-viscosity acrylic resin through its digital artery. The tissues around the vessels were then digested in hot alkali and the resulting casts treated for scanning electron microscopy. We concentrated on six different areas: the palmar and dorsal side of the skin, the eponychium, the perionychium, the nail bed and the nail root. On the palmar side, many vascular villi were evident: these capillaries followed the arrangement of the fingerprint lines, whereas on the dorsal side they were scattered irregularly inside the dermal papillae. In the hypodermal layer of the palmar area, vascular supports of sweat glands and many arteriovenous anastomoses were visible, along with glomerularshaped vessels involved in thermic regulation and tactile function. In the eponychium and perionychium, the vascular villi followed the direction of nail growth. In the face of the eponychium in contact with the nail, a wide-mesh net of capillaries was evident. In the nail bed, the vessels were arranged in many longitudinal trabeculae parallel to the major axis of the digit. In the root of the nail, we found many columnar vessels characterized by multiple angiogenic buttons on their surface.

show abstract

“…Afterward all the toes underwent a corrosive process in KOH solution (8%) at room temperature; the solution was changed every 12 hr for 4-5 days. The obtained casts, once cleared of tissues, were washed in distilled water and dissected under a stereomicroscope to obtain small specimens of different cutaneous areas and then prepared for SEM observation (Murakami, 1971;Hodde and Nowell, 1980;Miodonski et al, 1980;Weiger et al, 1986;Castenholz, 1989;Lametschwandtner et al, 1990;Rolf and Nilsson, 1992).…”

Section: Corrosion Casting Proceduresmentioning

confidence: 99%

The Cutaneous Microvascular Architecture of Human Diabetic Toe Studied by Corrosion Casting and Scanning Electron Microscopy Analysis

Sangiorgi

Manelli

Reguzzoni

et al. 2010

The Anatomical Record

View full text Add to dashboard Cite

In this morphological study, we report on the three-dimensional microvascular architecture constituting the toes of a patient affected by diabetic microangiopathy. We applied corrosion casting (CC) technique to the toes of a patient affected by Type 2 diabetes, who underwent surgery for explantation of inferior left limb due to necrotic processes of soft tissues. The toes of a foot traumatically explanted in a motorcycle accident were kept as controls. According to technical protocols, toes were injected with a low-viscosity acrylic resin (Mercox) through the major digital artery, tissues were corroded in KOH solution (8%), and resulting casts processed for SEM observations. Already at low magnification, in diabetic toes, we found an impairment of the linear track-like disposition of the vessels of plantar side, with signs of vascular disruption and obliterations, stopped resin, and leakages. Capillaries under the nail and a lot of vascular villi in eponychium and nail borders were damaged, and vascular regression phenomena acting on them were clearly visible. Resin leakages and impairment of normal vascular architecture were also observed in the root of the nail. This preliminary report represents only the first step for further investigations regarding morphological three-dimensional appearance of diabetic microangiopathy. CC and scanning electron microscopy technique well documented these morphological modifications, highlighting on both structural and ultrastructural features of diabetic toes microvessels. In conclusion, our qualitative data try to better focus on the pathophysiological mechanisms involved in diabetic dermopathy and microangiopathy, proposing CC as useful method to investigate on them.

show abstract

Scanning Electron Microscopic Studies on Blood Vessels in Cancer of the Larynx

Cited by 28 publications

References 17 publications

Angioarchitecture of squamous cell carcinoma from hamster buccal pouch: a scanning electron microscopy study

Angioarchitecture of squamous cell carcinoma from hamster buccal pouch: a scanning electron microscopy study

Microvascularization of the human digit as studied by corrosion casting

The Cutaneous Microvascular Architecture of Human Diabetic Toe Studied by Corrosion Casting and Scanning Electron Microscopy Analysis

Contact Info

Product

Resources

About