Scanning Electron Microscopic Studies of Tissue Elastin Components Exposed by a KOH-Collagenase or Simple KOH Digestion Method.

Ushiki, Tatsuo; Murakumo, Masashi

doi:10.1679/aohc.54.427

Cited by 51 publications

(30 citation statements)

References 25 publications

(3 reference statements)

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“…Other samples were first subjected to chemical and mechanical microdissection. For chemical microdissection, the specimens were digested either with HCl (Evan et al, 1976) or KOH (Ushiki and Murakumo, 1991). In both cases, collagenase digestion was omitted.…”

Section: Semmentioning

confidence: 99%

Renal corpuscle of the sturgeon kidney: An ultrastructural, chemical dissection, and lectin‐binding study

2003

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The sturgeon is an ancient species of fish that thrives in a wide range of ecological environments, from freshwater to seawater. Basic in this process of adaptation is the ability of the kidney to control fluid filtration and urine formation. However, the morphological basis of this process is mostly unknown. The aim of the present study was to use microdissection techniques (scanning electron microscopy (SEM), transmission electron microscopy (TEM), and lectin-binding histochemistry) to examine the structure of the renal corpuscle of the sturgeon Acipenser nacarii in order to reveal morphologic features that could be related to function, phylogeny, and habitat. The renal corpuscles are aligned along the intrarrenal arteries. The urinary pole shows a siphon-like neck segment (NS) in 92% of the nephrons, whose structural characteristics are different from those of other fish. The podocytes have cuboidal cellular bodies, intercellular contacts, and poorly developed cell processes. The podocyte glycocalyx contains N-acetylglucosamine and lacks sialic acid. The structural and lectin-binding patterns are similar to those found in the immature mammalian kidney. The glomerular basement membrane (GBM) is very thick and consists of three layers: a lamina rara externa, a lamina densa, and a thick subendothelial lamina. The latter contains tubular microfibrils, collagen fibers, and long mesangial cell processes. Frequently, the podocyte bodies attach directly to the GBM, and the area occupied by the filtration slits is very small. Furthermore, the GBM shows a glycosylation pattern different from that observed in most vertebrates. Contrary to what would be expected in sturgeons living in freshwater, the A. nacarii renal corpuscle morphology suggests a low glomerular filtration rate. Anat Rec Part A 272A: 563-573, 2003.

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Section: Semmentioning

confidence: 99%

Renal corpuscle of the sturgeon kidney: An ultrastructural, chemical dissection, and lectin‐binding study

2003

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“…The smallest observed spherules indicate that ϳ150 nm is the fundamental size in coacervation. Many researchers recognize 100 -200-nm fibrillar intermediates in native and artificial elastin (51)(52)(53)(54). At the late stage of crosslinked tropoelastin degradations that were seen here, particles were released that were similar to the size of the tropoelastin monomer.…”

Section: Discussionmentioning

confidence: 66%

Elastolytic Mechanism of a Novel M23 Metalloprotease Pseudoalterin from Deep-sea Pseudoalteromonas sp. CF6-2

Zhao

Chen

Xie

et al. 2012

Journal of Biological Chemistry

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Background:The mechanism of marine elastin degradation is unclear. Results: A novel M23 metalloprotease pseudoalterin from a marine bacterium degraded elastin by cleaving both the glycyl bonds and the peptide bonds involved in cross-linking. Conclusion: Pseudoalterin adopts a novel elastolytic mechanism different from other M23 metalloproteases. Significance: The results shed light on the mechanism of marine elastin degradation.

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“…Some authors state that the arrangement of elastic system fibers in the tissue are reflected in the functions they were due to play: support, elasticity, resilience, compliance, expansion or elastic retreat (De Carvalho & Line; CottaPereira et al, 1977;Kielty et al, 2002;Ross & Bornstein, 1969;Ushiki & Murakumo, 1991;Ushiki, 2002). In our study, the three types of elastic fibers in VUJ were observed, being dispersed throughout the muscle, thus associating this finding with its function and especially the internal ostium of the urethra, of elasticity, plasticity, expansion and quick retreat of urine flow.…”

Section: Discussionmentioning

confidence: 99%

Fibroelastic Components of the Vesicourethral Junction of Rats in the Aging Process

Reisdoerferer¹,

Pereira²,

Chopard³

2011

Int. J. Morphol.

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SUMMARY:The vesicourethral junction comprising the vesical trigone, is relevant in setting and positioning of the urinary bladder, along with the vesical neck, fixed by lateral ligaments of the bladder and tendinous arch of the pelvis fascia. Namely, the puboprostatic ligament (men) and the pubovesical (women). The circular set elastic fibers in this junction are important and valuable in the elasticity and plasticity of the area, allowing quick expansion and withdrawal with the flow of urine, and associated to smooth muscle tissue and nerve control form an important collective to maintain urinary continence. The objective of the present study is to describe the elastic system in the vesicouretral junction in relation to aging and its involvement in the states of urinary continence and incontinence, as well as the study of the vesicouretral junction in various age groups while evaluating with electron transmission microscopy. To carry out the study, 12 Wistar rats were used, divided into groups: neonate (4 animals), adult group (4 animals) and aged group (4 animals). Electron transmission microscopy with use of tanic acid technique associated to glutaraldehyde fixation, satisfactorily showed the extreme structural differences between mature elaunin and oxytalan fibers present between intercelular spaces and bundles of collagen fibers. The phases of elastogenesis in neonate animals and degradation of the elastic system of older animals were also evaluated.

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Scanning Electron Microscopic Studies of Tissue Elastin Components Exposed by a KOH-Collagenase or Simple KOH Digestion Method.

Cited by 51 publications

References 25 publications

Renal corpuscle of the sturgeon kidney: An ultrastructural, chemical dissection, and lectin‐binding study

Renal corpuscle of the sturgeon kidney: An ultrastructural, chemical dissection, and lectin‐binding study

Elastolytic Mechanism of a Novel M23 Metalloprotease Pseudoalterin from Deep-sea Pseudoalteromonas sp. CF6-2

Fibroelastic Components of the Vesicourethral Junction of Rats in the Aging Process

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