Scanning Electron Microscopic Observations on the 9-Day Opossum (&lt;i&gt;Didelphis virginian&lt;/i&gt;&lt;i&gt;a&lt;/i&gt;) Embryo

Krause, William J.; Cutts, J H

doi:10.1159/000145899

Cited by 8 publications

(2 citation statements)

References 4 publications

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“…Blebs have also been observed in physiologically healthy cells at particular stages of the cell cycle, such as when mesoderm cells migrate within the embryo 19 and in neurons differentiating in primary cultures 20 …”

Section: Discussionmentioning

confidence: 99%

Blebs in inner and outer hair cells: a pathophysiological hypothesis

et al. 2008

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Section: Discussionmentioning

confidence: 99%

Blebs in inner and outer hair cells: a pathophysiological hypothesis

et al. 2008

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“…Blebs also have been observed in physiologically healthy cells at particular stages of the cell cycle (3,17,27). As an example, when mesoderm cells migrate within the embryo, prominent blebs can be observed in situ (29). In addition, neurons differentiating in primary culture show circus movements, i.e., blebs circulating around the cell circumference (37).…”

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confidence: 99%

Na⁺ influx triggers bleb formation on inner hair cells

Shi

Gillespie²,

Nuttall³

2005

American Journal of Physiology-Cell Physiology

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Large blebs form rapidly on apical membranes of sensory inner hair cells (IHCs) when the organ of Corti is freshly isolated from adult guinea pigs. Bleb formation had two distinguishable phases. Initially, we identified small particles labeled with fluorescent annexin V; these rapidly coalesced into larger aggregates. After particle aggregation, a single membrane bleb emerged from cuticular plate at the vestigial kinocilium location, eventually reaching ∼10 μm maximum spherical diameter; blebs this size often detached from IHCs. Development of blebs was associated with elevated concentration of intracellular Na+; blocking Na+ influx through mechanotransduction and ATP channels in the apical pole of IHCs or by replacement of Na+ with N-methyl-d-glucamine prevented Na+ loading and bleb formation. Depletion of intracellular ATP, blocking cAMP synthesis, inhibition of vesicular transport with brefeldin A, or inhibition of phosphatidylinositol 3-kinase with 2-(4-morpholinyl)-8-phenyl-1(4 H)-benzopyran-4-one (LY-294002) significantly reduced bleb formation in the presence of a Na+ load. Neither the mechanism of blebbing nor the size growth of the IHC blebs was associated with cellular apoptosis or necrosis. Bleb formation was not significantly reduced by disassembling microtubules or decreasing intracellular hydrostatic pressure. Moreover, no polymerized actin was observed in the lumen of blebs. We conclude that IHC bleb formation differs from classic blebbing mechanisms and that IHC blebs arise from imbalance of endocytosis and exocytosis in the apical plasma membrane, linked to Na+ loading that occurs in vitro.

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The allantois of the north american opossum (didelphis virginiana) with preliminary observations on the yolk sac endoderm and trophectoderm

Krause

Cutts

1985

Anat. Rec.

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During the middle of prenatal day 10, the opossum allantois forms as a ventral outgrowth of the hindgut. By day 11 it appears as a large, fluid filled sac and by the middle of day 12 (just prior to birth) it reaches its maximal development. The simple squamous epithelium lining the allantois consists of only one cell type that often contains numerous filaments in the apical cytoplasm. At the luminal surface, the apices of the cells are united by junctional complexes and desmosomes are present between adjacent cells. The luminal surface is irregular, whereas laterally and basally the cell membranes show few if any infoldings. Mitotic figures and presumptive degenerating cells occasionally occur in the allantoic epithelium which rests on a delicate basal lamina. The allantois is covered by a simple squamous mesothelium that lacks a distinct basal lamina. Between the two epithelial sheets lie mesenchymal cells, collagen fibers, and blood vessels. No specializations of cell membranes were noted in either of the epithelial layers. The yolk sac endoderm consists of a single layer of squamous cells whose cytoplasm contains scattered profiles of rough endoplasmic reticulum and mitochondria. Extensive lateral and basal infoldings of the plasmalemma were not observed in these endodermal cells. The morphology of the trophectodermal (trophoblastic) cells indicates a cell type that is active in the transport of materials. Since the endodermal cells that line the allantois lack morphological features that would suggest the presence of mechanisms for transport or exchange, and because they remain relatively unchanged throughout pregnancy, it is thought that the allantois functions primarily in the storage of urinary wastes during prenatal life.(ABSTRACT TRUNCATED AT 250 WORDS)

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Scanning Electron Microscopic Observations on the 9-Day Opossum (<i>Didelphis virginian</i><i>a</i>) Embryo

Cited by 8 publications

References 4 publications

Blebs in inner and outer hair cells: a pathophysiological hypothesis

Blebs in inner and outer hair cells: a pathophysiological hypothesis

Na⁺ influx triggers bleb formation on inner hair cells

The allantois of the north american opossum (didelphis virginiana) with preliminary observations on the yolk sac endoderm and trophectoderm

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Scanning Electron Microscopic Observations on the 9-Day Opossum (<i>Didelphis virginian</i><i>a</i>) Embryo

Cited by 8 publications

References 4 publications

Blebs in inner and outer hair cells: a pathophysiological hypothesis

Blebs in inner and outer hair cells: a pathophysiological hypothesis

Na+ influx triggers bleb formation on inner hair cells

The allantois of the north american opossum (didelphis virginiana) with preliminary observations on the yolk sac endoderm and trophectoderm

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Na⁺ influx triggers bleb formation on inner hair cells