Scanning Electron Microscopic Observation of Pancreatic Ducts in WBN/Kob Rats

Ashizawa, Nobuo; Endoh, Hiroshi; Hidaka, Katsuko; Watanabe, Makoto; Fukumoto, Shiro

doi:10.1097/00006676-199511000-00011

Cited by 8 publications

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“…In various strains of rat, obesity and insulin resistance secondary to leptin receptor deficiency induce diabetes [7,11,17,19]. Original WBN/Kob male rats spontaneously develop diabetes, although its onset is secondary to chronic pan-pancreatitis [2,13,20,22,24,29]. In contrast, female rats of this strain do not show pancreatitis or diabetes.…”

Section: Introductionmentioning

confidence: 99%

“…In contrast, female rats of this strain do not show pancreatitis or diabetes. Male rats of this strain commonly develop chronic pancreatitis by the age of 3 months [2,13,20,22], while diabetes mellitus (characterized by hyperglycemia, hypoinsulinemia, and glycosuria) occurs at 9 months of age [20,22,29]. In WBN/ Kob-fatty rats, both females and males provide a model of non-insulin-dependent diabetes with obesity [1].…”

Section: Introductionmentioning

confidence: 99%

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The Influence of Dietary Restriction on the Development of Diabetes and Pancreatitis in Female WBN/Kob-Fatty Rats

et al. 2010

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Abstract:Original WBN/Kob male rats commonly develop chronic pancreatitis by the age of 3 months, while diabetes mellitus occurs at 9 months. In contrast, female rats of this strain do not show pancreatitis or diabetes. The WBN/Kob-fatty rat is a homozygous (fa/fa) congenic strain for the fa allele of the leptin receptor gene (Lepr). In WBN/Kob-fatty rats, both females and males provide a model of non-insulin-dependent diabetes with obesity. The leptin receptor fatty gene (Lepr fa ) induces obesity and hyperphagia. In the present study, we examined the effect of dietary restriction on pancreatitis and diabetes in female WBN/Kob-fatty rats. Five female fatty rats comprised a restricted feeding group with paired-feeding from 3 to 13 weeks of age, and five female lean rats comprised a control group with paired-feeding. At 13 weeks of age, two of the five female fatty rats of the control group developed diabetes mellitus, while no female fatty rats of the restricted feeding group developed diabetes mellitus. At this stage, pathological changes of the pancreas were observed in female fatty rats. All female fatty rats showed severe interlobular, intra-lobular and intra-islet fibrosis. In female fatty rats of the restricted feeding group, pathological changes of the pancreas were milder those of the free-feeding fatty group. Although dietary restriction could not completely prevent pancreatitis in female fatty rats, the development of diabetes was inhibited by its reduction of the severity of pancreatitis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Influence of Dietary Restriction on the Development of Diabetes and Pancreatitis in Female WBN/Kob-Fatty Rats

et al. 2010

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“…6). In male WBN/Kob rats, scanning and transmission electron microscopy revealed that pancreatic ductal lumina exhibit a winding form, and inner surfaces show long cilia and crater-like depressions with age (68), and that the cilia in pancreatic ducts are elongated without internal structural change (69).…”

Section: Development Of Genetically Diabetic Wbn/kob and Otsuka Long-mentioning

confidence: 99%

Exocrine Pancreatic Physiology

Ogami

Ōtsuki²

1998

Pancreas

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Japanese researchers have contributed to the development of various areas of pancreatic physiology such as hormonal and neural regulation of pancreatic exocrine secretion, effect of nutrients on the exocrine pancreas, and stimulus-secretion coupling in the exocrine pancreas. Among them, we selected three fruitful areas and reviewed them. These are in vitro experiment with the perfusion of the isolated rat pancreas, research related to the cholecystokinin (CCK)-releasing factor of pancreatic acinar cell origin (monitor peptide), and development and characterization of two genetically diabetic rats: WBN/Kob and OLETF (Otsuka Long-Evans Tokushima fatty) rats. PERFUSION OF ISOLATED PANCREASKanno (1) developed an elegant and fine method of perfusion of the rat isolated pancreas. In his study, the inlet of the vascular perfusion was the superior mesenteric artery, and the outlet was the portal vein. The common duct was constantly flushed. The inlet of perfusion of the common duct was the hepatic end of the duct, and the outlet was the duodenal end. This method was further modified to allow more effective perfusion of the vascuSummary: Exocrine pancreatic physiology has been actively investigated in Japan during the past 30 years. We selected three areas and reviewed them for this article. The selected areas are perfusion of the isolated pancreas, cholecystokinin (CCK)-releasing factor from pancreatic juice (monitor peptide), ~ 265 and genetically diabetic rats. The aim of this article is to present a brief overview of the selected areas of exocrine pancreatic physiology in Japan so that future research can be productively directed. Key Words: Exocrine pancreatic physiologyCholecystokinin-releasing factor-Genetically diabetic rats. lar system and simultaneous measuring of the flow rate of pancreatic juice ( 2 ) . The inlets of vascular perfusion were both the superior mesenteric artery and the celiac artery, whereas the outlet was the portal vein. During part of the experiment, the hepatic end of the common duct was ligated, and the pancreatic juice collected from the duodenal end after cannulation with a stainless steel tube (Fig. 1). By using this system of perfusion of the isolated pancreas, Kanno et al.(3) demonstrated that addition of exogenous insulin to the perfusate potentiates the action of CCK to increase both pancreatic-juice flow and amylase release and that raising the glucose concentration in the perfusate potentiates the CCK-induced exocrine secretary response through an increase in endogenous insulin release (Fig. 2 ) . Kanno et al. wrote many articles on the effects of hormones (3-5) and on other factors (2,619) on stimulus-secretion coupling in the exocrine perfused pancreas. Otsuki et al. (19) isolated and perfused rat pancreas according to the technique of Kanno with slight modifications. They measured not only pancreatic-juice flow and amylase output but also insulin (and glucagon) in the portal effluent. Eventually they studied simultaneously both the exocrine and endocrine responses in perfused i...

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“…The male WBN/Kob rat has been established as a model for chronic pancreatitis (Ohashi et al 1990; Ashizawa et al 1995). The pancreas of these animals undergoes focal changes of acinar apoptosis, inflammation, acinar-to-duct metaplasia with development of tubular complexes, and fibrosis.…”

Section: Introductionmentioning

confidence: 99%

Secretory apparatus assessed by analysis of pancreatic secretory stress protein expression in a rat model of chronic pancreatitis

Meili¹,

Graf

Perren³

et al. 2003

Cell and Tissue Research

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Secretory stress proteins (SSP) are a family of proteins including isoforms of pancreatitis-associated protein (PAP) and pancreatic stone protein (PSP/reg). In vitro exposure to trypsin results in the formation of insoluble fibrillar structures. SSP are constitutively secreted into pancreatic juice at low levels. The WBN/Kob rat is a model for chronic pancreatitis, displaying focal inflammation, destruction of the parenchyma and changes in the architecture of the acinar cell; the synthesis and secretion of SSP are also increased. We have investigated the secretory apparatus by SSP immunohistochemistry at the light- and electron-microscopical (EM) levels. Immunocytochemistry of PSP/reg in Wistar control rats reveals low levels, with individual acinar cells exhibiting high immunoreactivity in zymogen granules. PAP is not detectable. In the WBN/Kob rat, PSP/reg and PAP immunoreactivity is markedly increased. Double immunofluorescence for PSP/reg and PAP I or II demonstrates that these proteins colocalize to the same cell. Acinar cells change their secretory architecture by fusion of zymogen granules and elongation of the fused organelles. The immunogold technique has demonstrated an increase of SSP in zymogen granules in WBN/Kob rats. PSP/reg-positive zymogen granules fuse to form elongated structures with fibrillar contents. An extensive PSP/reg-positive fibrillar network is established in the cytosol. Extracellular fibrils have been observed in several ductules. Thus, SSP-derived fibrils form concomitantly with acinar damage in the WBN/Kob rat. Based on the known tryptic cleavage site of SSP, the in vivo generation of fibrils is presumably the result of premature trypsin activation.

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Scanning Electron Microscopic Observation of Pancreatic Ducts in WBN/Kob Rats

Cited by 8 publications

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The Influence of Dietary Restriction on the Development of Diabetes and Pancreatitis in Female WBN/Kob-Fatty Rats

The Influence of Dietary Restriction on the Development of Diabetes and Pancreatitis in Female WBN/Kob-Fatty Rats

Exocrine Pancreatic Physiology

Secretory apparatus assessed by analysis of pancreatic secretory stress protein expression in a rat model of chronic pancreatitis

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