Scanning electron microscopic observation of the architecture of collagen fibres in chicken M. iliotibialis lateralis

Abstract

1. The collagen architecture of M. iliotibialis lateralis in chicken was observed under the scanning electron microscope after muscle maceration in NaOH. 2. Immunohistochemical methods showed Type I and III collagens to be distributed over both perimysium and endomysium. 3. Thick perimysium around secondary myofibre fasciculi was composed of many large longitudinal collagen bundles and a few small circumferential bundles. In contrast, thin perimysium around primary myofibre fasciculi showed mainly circumferential bundles. 4. Endomysium had a honeycomb-like structure and consisted of a fine collagen mesh, its main fibre striation being circumferential. 5. It is suggested that functional demand differs between thick perimysium and thin endomysium.

Effects of high hydrostatic pressure on microstructure, texture, colour and biochemical changes of red abalone (Haliotis rufecens) during cold storage time

“…1a shows an unpressurised muscle of abalone sample whose endomysium (E) exhibited a honeycomb structure, surrounded by thin perymisium (P) around primary fascicule and thick perimysium around secondary fascicule. Pérez-Won, Tabilo-Munizaga, and Barbosa-Cánovas (2005) and Iwamoto et al (2001) obtained similar results for scallop adductor muscle and chicken muscle, respectively, in whose central area of the primary fascicule, some parts of the endomysium were thicker but still thinner than the thin perimysium.…”

Developmental states of the collagen content, distribution and architecture in thepectoralis,iliotibialis lateralisandpuboischiofemoralismuscles of male Red Cornish × New Hampshire and normal broilers

“…The large collagen content in the ITL muscle of Silkie cocks is attributable entirely to the development of perimysial collagen fibres. The well-developed collagen fibres in perimysium are also found in the ITL muscle of White Leghorn cocks (Iwamoto et al, 2001). In this study, regardless of the very close perimysial net, slow-twitch PIF material with fascia had the same collagen content as fast-twitch ITL material without fascia.…”

“…The collagen content, distribution and architecture in the PT, ITL and PIF muscles of broilers have been compared in the muscles of Silkie and White Leghorn cocks (Iwamoto et al, 2001;Nakamura et al, 2003b).…”

Histological Contribution of Collagen Architecture to Beef Toughness

“…The muscle materials were fixed in glutaraldehyde (20 mL/L)‐paraformaldehyde (20 mL/L) in 0.1 M phosphate buffer solution (pH 7.4) for several days at 4 °C and sliced transversely with a razor, followed by maceration in a 2 N sodium hydroxide solution for 4 to 5 d with methods slightly modified from those of Tabata and others (1995) and Iwamoto and others (2001). Macerated specimens were rinsed in distilled water for 3 d at 25 °C, treated with 10 mL/L tannic acid for 2 h and postfixed with osmium tetraoxide (10 mL/L) solution for 2 h. After dehydration in a graded ethanol series, specimens were placed in t‐butyl alcohol and freeze‐dried.…”