Scanning electron microscopic identification of <i>Sarcocystis gracilis</i> from roe deer and cattle

Stolte, M.; Bockhardt, I.; Odening, K.

doi:10.1111/j.1469-7998.1998.tb00030.x

Cited by 8 publications

(7 citation statements)

References 7 publications

(11 reference statements)

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“…Air-drying the cysts from HMDS at room temperature (all cysts depicted in Figs 2, 4 and 6) gave at least as good results by SEM as critical-point drying from CO 2 (cysts shown in Fig. 8), as also reported by Stolte et al (1996). Moreover, using HMDS allowed us to fixate, dehydrate and dry the tiny cysts while they were kept within the same Eppendorf tube, which prevented mechanical damage to, or loss of cysts during processing.…”

Section: Discussionsupporting

confidence: 82%

“…The protrusions were reported to be 6·7–8·3 μm long and 1·7–3·4 μm wide, which also suggests a mixture of 2 species similar to S. tarandi and S. rangiferi . The cysts from the captive wapiti were also depicted in Plate IV of a paper by Stolte et al (1996) and referred to as cysts of an unnamed Sarcocystis sp. In 1997, Stolte et al .…”

Section: Discussionmentioning

confidence: 98%

“…However, the given size descriptions of the cysts indicate a mixture of thin, slender cysts and fairly thick cysts, suggesting a mixed infection with S. rangiferi- like and S. tarandi- like cysts. Stolte et al (1996) compared the sarcocyst morphology of 11 species as seen in fresh preparations by LM and SEM. Plate V shows S. tarandi- like cysts from roe deer, which were classified as S. hofmanni .…”

Section: Discussionmentioning

confidence: 99%

“…8), as also reported by Stolte et al (1996). Moreover, using HMDS allowed us to fixate, dehydrate and dry the tiny cysts while they were kept within the same Eppendorf tube, which prevented mechanical damage to, or loss of cysts during processing.…”

Section: Preparation Of Sarcocysts For Semmentioning

confidence: 92%

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Molecular characterization of fiveSarcocystisspecies in red deer (Cervus elaphus), includingSarcocystis hjortin. sp., reveals that these species are not intermediate host specific

Dahlgren

Gjerde

2009

Parasitology

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Muscle tissue from 37 red deer from Norway was examined for sarcocysts. Sarcocysts from 2 reindeer were obtained for comparative studies. Cysts were excised and morphologically classified by light microscopy, scanning electron microscopy, and DNA sequence analysis. Five Sarcocystis species, Sarcocystis hjorti n. sp., Sarcocystis hardangeri, Sarcocystis ovalis, Sarcocystis rangiferi, and Sarcocystis tarandi, were found. All 5 species have previously been identified from either reindeer or moose by their sarcocyst morphology and/or ssu rRNA gene sequence. S. hjorti was the most prevalent species. Multiple variants of the ssu rRNA gene and the first internal transcribed spacer were found in S. rangiferi and S. tarandi from both red deer and reindeer. Phylogenetic analyses indicated that S. tarandi occurs in both red deer and reindeer, but it could not be clearly demonstrated whether the sequence variation within S. rangiferi between hosts was due to different paralogues or/and different species. DNA sequencing was necessary for definitive species identification, since the hair-like protrusions on the cysts of S. hjorti were not always recognizable by light microscopy and since different cervids harbour Sarcocystis species with highly similar cyst morphology of which at least some are not intermediate host specific.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Preparation Of Sarcocysts For Semmentioning

confidence: 92%

See 2 more Smart Citations

Molecular characterization of fiveSarcocystisspecies in red deer (Cervus elaphus), includingSarcocystis hjortin. sp., reveals that these species are not intermediate host specific

Dahlgren

Gjerde

2009

Parasitology

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“…hofmanni , were identified in European roe deer using light microscopy (LM), transmission electron microscopy (TEM), and combination thereof (Erber et al 1978; Entzeroth 1981, 1982; Enzeroth 1985; Sedlaczek and Wesemeier 1995; Santini et al 1997; Stolte et al 1996, 1998). The fourth unnamed Sarcocystis spp.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Sarcocystis species from Polish roe deer based on ssu rRNA and cox1 sequence analysis

2014

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Sarcocysts from four Polish roe deer were collected and examined by light microscopy, small subunit ribosomal RNA (ssu rRNA), and the subunit I of cytochrome oxidase (cox1) sequence analysis. This resulted in identification of Sarcocystis gracilis, Sarcocystis oviformis, and Sarcocystis silva. However, we were unable to detect Sarcocystis capreolicanis, the fourth Sarcocystis species found previously in Norwegian roe deer. Polish sarcocysts isolated from various tissues differed in terms of their shape and size and were larger than the respective Norwegian isolates. Analysis of ssu rRNA gene revealed the lack of differences between Sarcocystis isolates belonging to one species and a very low degree of genetic diversity between Polish and Norwegian sarcocysts, ranging from 0.1 % for Sarcocystis gracilis and Sarcocystis oviformis to 0.44 % for Sarcocystis silva. Contrary to the results of the ssu rRNA analysis, small intraspecies differences in cox1 sequences were found among Polish Sarcocystis gracilis and Sarcocystis silva isolates. The comparison of Polish and Norwegian cox1 sequences representing the same Sarcocystis species revealed similar degree of sequence identity, namely 99.72 % for Sarcocystis gracilis, 98.76 % for Sarcocystis silva, and 99.85 % for Sarcocystis oviformis. Phylogenetic reconstruction and genetic population analyses showed an unexpected high degree of identity between Polish and Norwegian isolates. Moreover, cox1 gene sequences turned out to be more accurate than ssu rRNA when used to reveal phylogenetic relationships among closely related species. The results of our study revealed that the same Sarcocystis species isolated from the same hosts living in different geographic regions show a very high level of genetic similarity.Electronic supplementary materialThe online version of this article (doi:10.1007/s00436-014-3966-x) contains supplementary material, which is available to authorized users.

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Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species

Dahlgren

Gjerde

2008

Parasitol Res

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Fresh muscle tissue from six roe deer from Southeastern Norway was examined for sarcocysts. Cysts were excised and morphologically classified by light microscopy, and some cysts were further examined by scanning electron microscopy or DNA amplification and sequencing of the small subunit (ssu) rRNA gene. Two Sarcocystis species, Sarcocystis gracilis and Sarcocystis oviformis n. sp., were found and described by morphological and molecular methods. S. gracilis was found in all animals, whereas S. oviformis was found in only one roe deer. Polymerase chain reaction identification was necessary for definitive species identification, since cysts of S. gracilis varied in surface structure and since cysts of both S. gracilis and S. oviformis were morphologically indistinguishable from sarcocysts in other cervidae. Phylogenetic analyses based on complete ssu rRNA gene sequences revealed a close relationship between S. gracilis and other canine-transmitted Sarcocystis species, whereas S. oviformis formed a well-supported group with Sarcocystis hardangeri of reindeer and Sarcocystis ovalis of moose.

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Scanning electron microscopic identification of Sarcocystis gracilis from roe deer and cattle

Cited by 8 publications

References 7 publications

Molecular characterization of fiveSarcocystisspecies in red deer (Cervus elaphus), includingSarcocystis hjortin. sp., reveals that these species are not intermediate host specific

Molecular characterization of fiveSarcocystisspecies in red deer (Cervus elaphus), includingSarcocystis hjortin. sp., reveals that these species are not intermediate host specific

Molecular characterization of Sarcocystis species from Polish roe deer based on ssu rRNA and cox1 sequence analysis

Sarcocystis in Norwegian roe deer (Capreolus capreolus): molecular and morphological identification of Sarcocystis oviformis n. sp. and Sarcocystis gracilis and their phylogenetic relationship with other Sarcocystis species

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