Scanning electrochemical microscopy based irreversible destruction of living cells

Poderyte, Margarita; Ramanavičius, Arūnas; Valiūnienė, Aušra

doi:10.1016/j.bios.2022.114621

Cited by 6 publications

(2 citation statements)

References 39 publications

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“…35 EIS can in-situ monitor cell damages, such as electroporation. 36 For the application of ECL imaging to living cells, the selection of suitable ECL chemicals and optimization of the measurement conditions is required. Recently, bipolar electrochemistry has been used for cell analyses, 37,38 which included the evaluation of cell adhesion.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Cell Adhesion Analysis Using Electrochemiluminescence Imaging and Electrochemical Impedance Spectroscopy

OBA,

INO,

UTAGAWA

et al. 2024

Electrochemistry

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Cell adhesion to culture substrates plays a crucial role in cellular activities, such as proliferation, differentiation, and apoptosis. Recently, electrochemiluminescence (ECL) imaging has been utilized for analyzing cell adhesion. In addition to ECL imaging, electrochemical impedance spectroscopy (EIS) is widely used as a conventional method for evaluating cell adhesion. However, the relationship between the results obtained from ECL imaging and EIS has not yet been investigated. In this study, the relationship between the results obtained using two methods is explored, and their advantages and disadvantages have been discussed. Endothelial cells were cultured on an extracellular matrix-coated indium tin oxide electrode for 24 h, which was further used for ECL imaging and EIS. To the best of our knowledge, this is the first report of ECL imaging and EIS of the same samples for cell adhesion analysis. This comprehensive analysis of cell adhesion using EIS and ECL imaging could be further used to evaluate drugs that target cell adhesion.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Cell Adhesion Analysis Using Electrochemiluminescence Imaging and Electrochemical Impedance Spectroscopy

OBA,

INO,

UTAGAWA

et al. 2024

Electrochemistry

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“…[ 5 ] The common methods for characterizing these parameters (e.g., fluorescence method and flow cytometry) are associated with complicated processes, characterization of only a single parameter, and labeling of target molecules which may alter the cellular state, making it challenging to capture the dynamic physiological and metabolic changes of neurons in real time. [ 6 ] Scanning electrochemical microscopy (SECM), a label‐free and noninvasive electrochemical tool capable of in situ recording various redox processes around a cell utilizing a µm or nm‐sized electrode as its probe, has been widely used in living cell studies including imaging cell morphology, [ 7 ] monitoring cell‐released chemicals, [ 8 ] regulating cell survival, [ 9 ] and inspecting cellular redox activity and metabolism. [ 10 ] The recently developed programmable‐SECM mode applying pulse potentials can further realize the multi‐target detection during a single scan, shorten the detection time of multiple analytes, and minimize the charging current influence generated by a potential switch on the Faradic current signal.…”

Section: Introductionmentioning

confidence: 99%

In Situ and Quantitatively Imaging of Heat‐Induced Oxidative State and Oxidative Damage of Living Neurons Using Scanning Electrochemical Microscopy

Zhang

Liu

et al. 2022

Small Methods

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Central nervous system is sensitive and vulnerable to heat. Oxidative state and oxidative damage of neurons under heat stress are vital for understanding early consequences and mechanisms of heat‐related neuronal injury, which remains elusive partly due to the technical challenge of in situ and quantitative monitoring methods. Herein, a temperature‐controlled scanning electrochemical microscopy (SECM) platform with programmable pulse potential and depth scan modes is developed for in situ and quantitatively monitoring of oxygen consumption, extracellular hydrogen peroxide level, and cell membrane permeability of neurons under thermal microenvironment of 37–42 °C. The SECM results show that neuronal oxygen consumption reaches a maximum at 40 °C and then decreases, extracellular H2O2 level increases from 39 °C, and membrane permeability increases from 2.0 ± 0.6 × 10−5 to 7.2 ± 0.8 × 10−5 m s−1 from 39 to 42 °C. The therapeutic effect on oxidative damage of neurons under hyperthermia conditions (40–42 °C) is further evaluated by SECM and fluorescence methods, which can be partially alleviated by the potent antioxidant edaravone. This work realizes in situ and quantitatively observing the heat‐induced oxidative state and oxidative damage of living neurons using SECM for the first time, which results can contribute to a better understanding of the heat‐related cellular injury mechanism.

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Scanning electrochemical microscopy for the stimulation and investigation of human skeletal muscle-derived mesenchymal stem/stromal cells

Bironaitė

Petroniene

Mikšiūnas

et al. 2023

Electrochimica Acta

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Scanning electrochemical microscopy based irreversible destruction of living cells

Cited by 6 publications

References 39 publications

Comprehensive Cell Adhesion Analysis Using Electrochemiluminescence Imaging and Electrochemical Impedance Spectroscopy

Comprehensive Cell Adhesion Analysis Using Electrochemiluminescence Imaging and Electrochemical Impedance Spectroscopy

In Situ and Quantitatively Imaging of Heat‐Induced Oxidative State and Oxidative Damage of Living Neurons Using Scanning Electrochemical Microscopy

Scanning electrochemical microscopy for the stimulation and investigation of human skeletal muscle-derived mesenchymal stem/stromal cells

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