Scanning electrochemical microscopy based evaluation of influence of pH on bioelectrochemical activity of yeast cells − Saccharomyces cerevisiae

Ramanavičius, Arūnas; Morkvėnaitė-Vilkončienė, Inga; Kisieliute, Aura; Petroniene, Jurate; Ramanavičienė, Almira

doi:10.1016/j.colsurfb.2016.09.039

Cited by 19 publications

(8 citation statements)

References 22 publications

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“…In a SECM experiment the current is registered by the UME in culture medium containing MD at varying distances from the immobilized cells (Scheme ). MD diffuses through the cell membrane and is reduced by an intracellular nicotinamide adenine dinucleotide phosphate (NAD(P) NAD(P)H)‐depending enzymes that transfer two electrons and two protons to MD . The reduced form of MD – menadiol (MH 2 ) is a lipophilic compound, which can diffuse from the intracellular part of the cell to the extracellular solution.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Redox Activity of Human Myocardium‐derived Mesenchymal Stem Cells by Scanning Electrochemical Microscopy

Petroniene

Morkvėnaitė-Vilkončienė

Mikšiūnas

et al. 2020

Electroanalysis

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In this study the redox activity of human myocardium‐derived mesenchymal stem cells (hmMSC) were investigated by redox‐competition (RC‐SECM) and generation‐collection (GC‐SECM) modes of scanning electrochemical microscopy (SECM), using 2‐methylnaphthalene‐1,4‐dione (menadione, MD) as a redox mediator. The redox activity of human healthy and dilated hmMSCs was evaluated by measuring reduction of MD. Measurements were performed by approaching and retracting the UME from the surface of growing hmMSC cells. The current study shows that the RC‐SECM mode can be applied to investigate integrity of cell membranes, whereas the most promising results were observed by using the GC‐SECM mode and applying the Hill's equation for the calculation/fitting of dependencies of electrical current vs menadione concentration. The calculated apparent Michaelis constant (KM) for the production of menadiol (MDH2) in the pathological hmMSC cells was 14.4 folds higher compared to that of the healthy hmMSC revealing the lover redox activity of pathological cells. Moreover, the calculated Hill's coefficient n shows a negative cooperative binding between MD and healthy hmMSC and positive cooperative binding between MD and pathological hmMSC. It means that healthy hmMSC is of lower affinity to MD, which is also related to the better membrane integrity of healthy cells. Data of this study demonstrate that SECM can be applied to investigate intracellular redox and membrane changes ongoing in human dilated myocardium‐derived hmMSC in order to improve their functioning and further regenerative potential.

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Section: Resultsmentioning

confidence: 99%

Evaluation of Redox Activity of Human Myocardium‐derived Mesenchymal Stem Cells by Scanning Electrochemical Microscopy

Petroniene

Morkvėnaitė-Vilkončienė

Mikšiūnas

et al. 2020

Electroanalysis

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“…This approach has been used as a bioanalytical technique for monitoring cellular homeostasis through the evaluation of living cell membrane permeability to a series of compounds such as toxic heavy metals [128–130] . AFM‐SECM is also useful for the study of living single‐cell microorganisms such as bacteria, providing insights into metabolic exchange between two different bacteria [131] or even furnishing information on the efficiency of charge transfer from yeast cells under anaerobic conditions [132] . This approach was employed by Nebel et al [133] .…”

Section: Biospectroelectrochemistry Through Membranes and Cellsmentioning

confidence: 99%

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

et al. 2020

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When bioelectrochemistry meets other analytical techniques for in situ experiments, new possibilities for understanding the nature of charge‐transfer reactions are provided. Despite progress in recent years in analytical instrumentation, a number of key issues remains for bioelectrochemistry, in terms of the mechanistic description of the surface reactions involved in biomolecules’ electron transfer. The main challenges of these techniques are the concomitant detection of electron transfer and molecular alteration as well as the development of suitable bioelectrodes. This review discusses the most recent and emerging in situ and operando electrochemical methods used to study biological systems such as redox proteins, nucleic acids, small biomolecules, cells, and biomembranes, focusing on electrochemical methods coupled with spectroscopic, spectrometric, and microscopic techniques.

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“…SECM study entries into the literature databases that underline well the wide applicability of scanning electrochemical probe-based cellular analysis are examinations of the differentiation status of embryonic stem cells [72], evaluation of the respiration activity of blastocysts [73], monitoring intracellular enzyme activity of HeLa cells [74], analysis of beat fluctuations and oxygen consumption of cardiomyocytes [75], characterization of Ag + toxicity on fibroblast cells [76], inspection of the multi-drug resistance of adenocarcinoma cervical cancer cells [77], the detection of reactive oxygen species in prostate cancer cells [78], the creation of insulin release profiles of single pancreatic islets [79] and the disclosure of Cd 2+ -induced variability in the permeation properties of human bladder cancer cells [80,81]. Recent activities also addressed topics such as SECM-based inspections of heavy metal (chromium) cell toxicity [82,83], quantitative detection of protein markers for lung cancer [84], real-time monitoring of metabolic crosstalk between neighbouring bacterial cells [85], spatial mapping of molecular uptake of chemical species at living cells [59] and the pH dependence of yeast cell viability and metabolism [66].…”

Section: Scanning Electrochemical Microscopy-based Live Cell Imaging:mentioning

confidence: 99%

Biological imaging with scanning electrochemical microscopy

2018

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Scanning electrochemical microscopy (SECM) is a powerful and versatile technique for visualizing the local electrochemical activity of a surface as an ultramicroelectrode tip is moved towards or over a sample of interest using precise positioning systems. In comparison with other scanning probe techniques, SECM not only enables topographical surface mapping but also gathers chemical information with high spatial resolution. Considerable progress has been made in the analysis of biological samples, including living cells and immobilized biomacromolecules such as enzymes, antibodies and DNA fragments. Moreover, combinations of SECM with complementary analytical tools broadened its applicability and facilitated multi-functional analysis with extended life science capabilities. The aim of this review is to present a brief topical overview on recent applications of biological SECM, with particular emphasis on important technical improvements of this surface imaging technique, recommended applications and future trends.

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Scanning electrochemical microscopy based evaluation of influence of pH on bioelectrochemical activity of yeast cells − Saccharomyces cerevisiae

Cited by 19 publications

References 22 publications

Evaluation of Redox Activity of Human Myocardium‐derived Mesenchymal Stem Cells by Scanning Electrochemical Microscopy

Evaluation of Redox Activity of Human Myocardium‐derived Mesenchymal Stem Cells by Scanning Electrochemical Microscopy

In Situ and Operando Techniques for Investigating Electron Transfer in Biological Systems

Biological imaging with scanning electrochemical microscopy

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