Scanning cytophotometric analysis of myocardial nucleic acid and chromatin changes in soman toxicated rabbits

Martin, Lee J.; Doebler, Jeffrey A.; Swisher, J.; Anthony, Adam; Shih, Tsung‐Ming

doi:10.1002/cbf.290020410

Cited by 5 publications

(3 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The basis for this stems from extensive studies, indicating that expansion of the chromatin template is frequently associated with an increase in the acid lability of the chromatin and increased staining. This has been described as the chromatin activation reaction that is indicative of a heightened metabolic state (Martin et al, 1984). The increased RNA transcriptional activity of the SHR was biochemically documented in isolated myocyte (pooled and regional) RNA content of the 13-weekold animals.…”

Section: Discussionmentioning

confidence: 99%

Age-related changes in ploidy levels and biochemical parameters in cardiac myocytes isolated from spontaneously hypertensive rats.

Engelmann¹,

Vitullo²,

Gerrity³

1986

Circulation Research

View full text Add to dashboard Cite

Postnatal growth of the mammalian ventricular myocyte is characterized by a brief period of hyperplasia followed by an extensive period of physiological hypertrophy. Using myocytes isolated from both Wistar-Kyoto and spontaneously hypertensive rats from fetus to 10 months old, we analyzed morphological, biochemical, and ploidy changes. Fetal myocytes from both spontaneously hypertensive and Wistar-Kyoto rats were mononuclear, diploid cells. By 4 weeks, adult binucleation levels (84% binuclear) were found, and myocytes pooled from both ventricles demonstrated nuclear ploidy shifts to tetraploid levels. However, analysis of myocytes isolated from left ventricle, right ventricle, and septum showed that nuclear polyploidation was confined to the right ventricle and septum, with few polyploid nuclei detected in the left ventricle. This pattern remained relatively constant through 10 months of age, although spontaneously hypertensive myocytes showed significantly more polyploidation than Wistar-Kyoto in all regions. Biochemical analysis of isolated myocytes substantiated the nuclear ploidy changes and demonstrated elevated protein and ribonucleic acid content in left ventricular myocytes without extensive polyploidation. Since cardiac hypertrophy, both physiological and pathological, is associated primarily with the left ventricle and occurs in the absence of significant ploidy changes, these findings suggest that a unique pattern of gene regulation may be ongoing in the left ventricle myocytes that is not present in the septum and right ventricle. These variations may be essential for cellular hypertrophy under normal and pathological conditions.

show abstract

Section: Discussionmentioning

confidence: 99%

Age-related changes in ploidy levels and biochemical parameters in cardiac myocytes isolated from spontaneously hypertensive rats.

Engelmann¹,

Vitullo²,

Gerrity³

1986

Circulation Research

View full text Add to dashboard Cite

show abstract

“…An increase in Feulgen reactivity is thus indicative of alterations in the deoxyribonucleoprotein (DNP) complex and subsequent availability of aldehyde groups. This "chromatin activation reaction" has been used as an index of general template activity (17,18). Since histamine is known to affect both growth and metabolism of arterial cells, the characterization of histamine receptors with respect to indices of DNA content and chromatin dispersion was of interest.…”

Section: -Methyihistamine Dimaoritmentioning

confidence: 99%

Histamine receptor-mediated changes in nuclear chromatin of cultured endothelial and smooth muscle cells.

Blank

Hollis

1986

Acta Histochem. Cytochem

View full text Add to dashboard Cite

“…Procedures used in cytophotometric DNA and RNA analyses were as described previously (6, [10][11][12]. Briefly, 15-pm paraffin sections were stained for DNA with the Feulgen nucleal reaction ( 13) and for RNA with azure B (14).…”

mentioning

confidence: 99%

Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats

Martin

Doebler

Anthony

1986

Experimental Biology and Medicine

View full text Add to dashboard Cite

Neuronal nucleic acid responses were examined within the rat striatum and sensorimotor cortex (layer V) following single intraperitoneal injections of the central cholinergicmuscarinic agonist oxotremorine (0.1, 0.7, or 1.0 mg/kg). After stoichiometric Feulgen and azure B staining of brain sections, scanning-integrating microdensitometry was used to quantify Feulgendeoxyribonucleic acid levels, changes in the susceptibility of chromatin to Feulgen acid hydrolysis (F-DNA yield) and azure B-ribonucleic acid (RNA) content of neurons on an individual basis. Changes in neuronal nuclear and nucleolar volumes were also determined histometrically. Within the striatum and sensorimotor cortex, oxotremorine produced marked dose-dependent elevations in both F-DNA yield and RNA content. These metabolic increases were typically paralleled by elevations in nuclear and nucleolar volumes. The data demonstrate that the oxotremorine-induced central muscarinic activation is associated with dose-related enhancements in neuronal chromatin template activity, RNA content, and protein synthetic capacity.

show abstract

Scanning cytophotometric analysis of myocardial nucleic acid and chromatin changes in soman toxicated rabbits

Cited by 5 publications

References 13 publications

Age-related changes in ploidy levels and biochemical parameters in cardiac myocytes isolated from spontaneously hypertensive rats.

Age-related changes in ploidy levels and biochemical parameters in cardiac myocytes isolated from spontaneously hypertensive rats.

Histamine receptor-mediated changes in nuclear chromatin of cultured endothelial and smooth muscle cells.

Cytophotometric Analysis of Neuronal Chromatin and RNA Changes in Oxotremorine-Treated Rats

Contact Info

Product

Resources

About