Scanning amplicons with CRISPR‐Dx detects endangered amphibians in environmental DNA
Flurin Leugger,
Michel Schmidlin,
Martina Lüthi
et al.
Abstract:More efficient methods for extensive biodiversity monitoring are required to support rapid measures to address the biodiversity crisis. While environmental DNA (eDNA) metabarcoding and quantitative PCR (qPCR) methods offer advantages over traditional monitoring approaches, their large‐scale application is limited by the time and labour required for developing assays and/or for analysis. CRISPR (clustered regularly interspaced short palindromic repeats) diagnostic technologies (Dx) may overcome some of these li… Show more
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