Scaly green flagellates from Spanish Atlantic coastal waters: molecular, ultrastructural and pigment analyses

Alonso-González, Aitor; Orive, Emma; David, Helena; García-Etxebarria, Koldo; Garrido, J. L.; Laza‐Martínez, Aitor; Seoane, Sergio

doi:10.1515/bot-2013-0108

Cited by 8 publications

(6 citation statements)

References 72 publications

(120 reference statements)

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“…To overcome this problem, Garrido, Rodríguez & Zapata () proposed a new method and a double analysis procedure that has been recently employed to characterize the composition of scaly green flagellates (Alonso‐González et al . ). The HPLC method here proposed, with all common pigments completely separated (Loroxanthin and Neoxanthin Rs > 1·5), is a good option for a re‐examination of the still doubtful xanthophyll distribution in the prasinophytes.…”

Section: Discussionmentioning

confidence: 97%

Phytoplankton pigment biomarkers: HPLC separation using a pentafluorophenyloctadecyl silica column

et al. 2015

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Summary The use of pigment data to map microalgal populations in natural waters has become an established and convenient way of studying phytoplankton. However, the chromatographic analysis of algal pigments is a major challenge due to the diversity of molecules spanning a wide range of polarities, but also including many that have closely similar chemical structures. Among these sets of compounds of similar structure, the separation of mono and divinylic pairs of chlorophylls is of particular importance due to their relevance as chemotaxonomical biomarkers. In this work, we have taken advantage of the special type of solute–stationary phase interactions provided by pentafluorophenyl phases together with the high retention values resulting from an octadecyl spacer in the column to develop a method for the joined analysis of chlorophylls and carotenoids. The mobile phase contains organic solvents of low toxicity, methanol and ethanol and ammonium acetate buffer, in a simple binary elution gradient. More than seventy photosynthetic pigments (chlorophylls, carotenes and xanthophylls) can be determined in the same chromatographic analysis employing the method here presented. The complete resolution of mono and divinylic forms of chlorophylls a, b and c is achieved in less than 42 min. The same analysis allows the separation of most chemotaxonomically important carotenoids, including positional isomers. The method can successfully be applied to the characterization of the pigment composition of members of different classes in the main chloroplast lineages (red and green) of the evolution of photosynthetic eukaryotes, in the study of biosynthetic processes of chlorophylls and especially in the description of plankton populations in natural waters. It is particularly suited for the simultaneous detection of green algae and the cyanobacteria of outstanding global importance Prochlorococcus marinus.

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Section: Discussionmentioning

confidence: 97%

Phytoplankton pigment biomarkers: HPLC separation using a pentafluorophenyloctadecyl silica column

et al. 2015

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“…Although size is not enough for species delimitation, the large Pyramimonas cells observed during this study (15-23 µm long and 10-17.5 µm wide) had closer dimensions to P. gelidicola. The identification at species level of Pyramimonas is mainly based on the morphology of the scales that cover their body (Norris and Pienaar 1978;McFadden et al 1986;Hori et al 1995;Alonso-González et al 2014), based on transmission electron microscopy (TEM) observations. Unfortunately, only a few crown scales were observed during our SEM observations, which were not enough for species identification.…”

Section: Pyramimonas Sp (Prasinophyceae)mentioning

confidence: 99%

Phytoplankton composition and bloom formation in unexplored nearshore waters of the western Antarctic Peninsula

et al. 2019

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The western Antarctic Peninsula (WAP) is one of the most productive regions in the Southern Ocean. However, little is known about the phytoplankton composition in nearshore waters, in fjords and channels between 63º and 67°S, where Antarctic krill and baleen whales are conspicuous. This study represents the first attempt to describe spatial and temporal composition of the phytoplankton community (species, cell concentration, phytoplankton biomass) in twelve relatively unexplored nearshore sites of the WAP. Sampling was carried out in the frame of a Citizen Science project during late summer of 2016 and during the spring-summer 2016-2017. Species identification and enumeration were performed by light and scanning electron microscopy and phytoplankton carbon biomass was estimated by using cell-volume conversion. The highest phytoplankton abundance and biomass values were found in December-January, and were mainly represented by nanophytoflagellates (2-20 µm). Cryptophytes were more abundant in early summer and prasinophyceans in late summer. The abundance of large bloom-forming diatoms was unexpectedly low. Three blooming flagellated taxa were found during the sampling season, chronologically:

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“…Their structural variety and beneficial activities are attractive in the food and pharmaceutical industries. The pigment analysis of seven prasinophyceans (Chlorophyta) distinguished three groups that contained prasinoxanthin, loroxanthin ester and siphonaxanthin, respectively [ 42 ]. The bioactive compound analysis revealed that Nannochloropsis sp.…”

Section: Chemistry and Biochemistry Of Microalgal Pigmentsmentioning

confidence: 99%

Microalgae-Derived Pigments for the Food Industry

Sun

Wang

et al. 2023

Marine Drugs

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In the food industry, manufacturers and customers have paid more attention to natural pigments instead of the synthetic counterparts for their excellent coloring ability and healthy properties. Microalgae are proven as one of the major photosynthesizers of naturally derived commercial pigments, gaining higher value in the global food pigment market. Microalgae-derived pigments, especially chlorophylls, carotenoids and phycobiliproteins, have unique colors and molecular structures, respectively, and show different physiological activities and health effects in the human body. This review provides recent updates on characteristics, application fields, stability in production and extraction processes of chlorophylls, carotenoids and phycobiliproteins to standardize and analyze their commercial production from microalgae. Potential food commodities for the pigment as eco-friendly colorants, nutraceuticals, and antioxidants are summarized for the target products. Then, recent cultivation strategies, metabolic and genomic designs are presented for high pigment productivity. Technical bottlenecks of downstream processing are discussed for improved stability and bioaccessibility during production. The production strategies of microalgal pigments have been exploited to varying degrees, with some already being applied at scale while others remain at the laboratory level. Finally, some factors affecting their global market value and future prospects are proposed. The microalgae-derived pigments have great potential in the food industry due to their high nutritional value and competitive production cost.

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Scaly green flagellates from Spanish Atlantic coastal waters: molecular, ultrastructural and pigment analyses

Cited by 8 publications

References 72 publications

Phytoplankton pigment biomarkers: HPLC separation using a pentafluorophenyloctadecyl silica column

Phytoplankton pigment biomarkers: HPLC separation using a pentafluorophenyloctadecyl silica column

Phytoplankton composition and bloom formation in unexplored nearshore waters of the western Antarctic Peninsula

Microalgae-Derived Pigments for the Food Industry

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