Scalp Induction Rate Responses to Cytokinins on Proliferating Shoot-Tips of Banana Cultivars (&lt;i&gt;Musa&lt;/i&gt; spp.)

Shirani, Siamak; Meon, Sariah; Zakaria, W.; Mahmood, Maziah

doi:10.3844/ajabssp.2010.128.134

Cited by 11 publications

(5 citation statements)

References 20 publications

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“…Hence Kn can have no effect on the shoot multiplication of both banana varieties. This observation is in correlation with the findings of Shirani et al (2010). In contrast, Muhammad et al (2007) have reported that the number of shoot formation increases with increase in Kn concentration up to a certain level after that higher concentration will decrease the formation of multiple shoots.…”

Section: Variable Responses Of Two Banana Varieties 97supporting

confidence: 89%

Variable Responses of Two Banana Varieties during in vitro Multiplication

Krishna¹,

Sabitha²,

Gayathri³

et al. 2022

Plant Tissue Cult. & Biotech.

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Banana is the choicest crop for the micropropagation industries due to the recurrent requirement of large volume of plants in the domestic market. However, all the banana varieties cannot be cultured in media with the same composition to get the optimum shoots in multiplication cycleswith desirable shoot qualities. Banana varieties like Robusta grow optimally in Murashige and Skoog medium containing 3-5 mg/L BA (6- benzyl adenine). Under the same condition, the growth of Nendran is found to be poor with a low number of rootable shoots. The poor multiplication ratio causes a higher cost for the micropropagated plantlets of var. Nendran. Hence the current study was carried out to optimize the shoot multiplication of the two varieties of banana (Nendran and Robusta) in MS medium supplemented with various combinations and concentrations of plant growth regulators. The multiplying shoots were cultured in MS medium supplemented with different concentrations of BA, combinations of BA, NAA (- naphthalene acetic acid) and combinations of KN (kinetin) and NAA. Combinations of BA and NAA (0.5 mg/L BA+0.1 mg/L NAA or 0.5mg/L BA+0.5 mg/L NAA) was found to result in responses ideal for commercial production of var. Nendran. For the var. Robusta, 2 mg/L BA was ideal for shoot multiplication and 1 mg/L BA+0.5 mg/L NAA could be optimal for use at elongation stage to get maximum desirable shoots for rooting. The study could optimize a suitable plant growth regulator regime for the commercial production of banana var. Nendran and could impact cost reduction of plantlets. Plant Tissue Cult. & Biotech. 32(1): 89-101, 2022 (June)

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Section: Variable Responses Of Two Banana Varieties 97supporting

confidence: 89%

Variable Responses of Two Banana Varieties during in vitro Multiplication

Krishna¹,

Sabitha²,

Gayathri³

et al. 2022

Plant Tissue Cult. & Biotech.

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“…Scalps, a cauliflower-like structure, are actively proliferating meristems, derived from shoot tip culture and appear as a white bulbous structure and have been reported by several researchers in monocot plants, especially banana [ 41 ]. It was also reported in C. orchioides as a bulbil or bulblet [ 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

An EfficientIn VitroPlantlet Regeneration from Shoot Tip Cultures ofCurculigo latifolia, a Medicinal Plant

Babaei

Abdullah

Saleh

et al. 2014

The Scientific World Journal

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A procedure was developed for in vitro propagation of Curculigo latifolia through shoot tip culture. Direct regeneration and indirect scalp induction of Curculigo latifolia were obtained from shoot tip grown on MS medium supplemented with different concentrations and combinations of thidiazuron and indole-3-butyric acid. Maximum response for direct regeneration in terms of percentage of explants producing shoot, shoot number, and shoot length was obtained on MS medium supplemented with combination of thidiazuron (0.5 mg L−1) and indole-3-butyric acid (0.25 mg L−1) after both 10 and 14 weeks of cultures. Indole-3-butyric acid in combination with thidiazuron exhibited a synergistic effect on shoot regeneration. The shoot tips were able to induce maximum scalp from basal end of explants on the medium with 2 mg L−1 thidiazuron. Cultures showed that shoot number, shoot length, and scalp size increased significantly after 14 weeks of culture. Transferring of the shoots onto the MS medium devoid of growth regulators resulted in the highest percentage of root induction and longer roots, while medium supplemented with 0.25 mg L−1 IBA produced more numbers of roots.

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“…The phenomenon of genotype dependency in response to in vitro date palm manipulations is well documented in various plant species (Karami, 2008;Shirani et al, 2010) as well as date palm (Al-Khayri and Al-Bahrany, 2004). The results have shown that the effect of basal media formulations can be influenced by various date palm genotypes.…”

Section: Discussionmentioning

confidence: 86%

Basal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic Embryogenesis

Al-Khayri¹

2011

American Journal of Biochemistry and Biotechnology

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Problem statement: Various media formulations differing in basal salt composition are arbitrary selected to provide essential nutrients for plant in vitro cultures. Evidence suggests that in vitro response to various media formulations varies among genotypes and depends on the culture stage. This study examined the efficacy of five basal salt formulations on callus growth and somatic embryogenesis in date palm Phoenix dactylifera L. using three commercial cultivars, Khusab, Berny and Barhee. Approach: Callus from shoot tip explants maintained on MS medium was introduced to various media formulations including SH, W, MS, WPM and NN media containing 53.7 μM NAA and 7.4 μM 2iP. To assess the effect on callus growth, fresh callus weight was measured 4 and 8 weak later. To evaluate embryogenesis response, callus was transferred to hormone-free media corresponding to those during callus growth stage. Results: The optimum medium formulation varied according to cultivar and culture stage. Extending callus growth to 8 weak allowed for greater discernment of differences as compared to 4 weak because of the inherent slow growth nature of date palm callus. The best callus growth was achieved in cv. Khusab using W and WPM media, cv. Berny using SH and NN medium and cv. Barhee using SH, W and MS media. An optimal medium for callus growth was not necessarily the best for somatic embryogenesis. The highest regeneration percentage in cv. Berny occurred on WPM medium, cv. Khusab on W medium and cv. Barhee using W and WPM media. The highest number of resultant embryos was achieved in cv. Khusab using W and SH media, cv. Berny on WPM and MS media and cv. Barhee using W and SH media. Conclusion: This study provides important information to optimize medium formulation in micropropagation protocols of various date palm cultivars, particularly recalcitrant genotypes. It showed that the best basal salt formulation differed among date palm genotypes and suggested culture stage-specific requirement.

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Scalp Induction Rate Responses to Cytokinins on Proliferating Shoot-Tips of Banana Cultivars (<i>Musa</i> spp.)

Cited by 11 publications

References 20 publications

Variable Responses of Two Banana Varieties during in vitro Multiplication

Variable Responses of Two Banana Varieties during in vitro Multiplication

An EfficientIn VitroPlantlet Regeneration from Shoot Tip Cultures ofCurculigo latifolia, a Medicinal Plant

Basal Salt Requirements Differ According to Culture Stage and Cultivar in Date Palm Somatic Embryogenesis

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