Scaling-up production and field performance of micropropagated medicinal herb ‘Safed Musli’ (Chlorophytum borivilianum)

Dave, Ashish; Bilochi, Geeta; Purohit, S. D.

doi:10.1079/ivp2003431

Cited by 24 publications

(12 citation statements)

References 10 publications

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“…Cultures were established using stem disc explant containing shoot buds as reported by Dave et al (2003). Shoots sprouted from the buds were grown and subsequently maintained on MS medium supplemented with 5.0 μM BA for at least 2 -3 cycles before experimentation.…”

Section: Resultsmentioning

confidence: 99%

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Kumar¹,

Aggarwal²,

Gupta³

et al. 2010

Biologia plant.

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The effect of plant growth regulators (PGRs), gelling agents, sucrose and heat shock on shoot multiplication, shoot growth, rooting and subsequent survival of Chlorophytum borivilianum Sant. et Fernand was evaluated. Benzyladenine (BA) was found to be better cytokinin over kinetin (KIN) for shoot multiplication. Sucrose concentrations from 116 -290 mM in the basal medium (BM) promoted shoot multiplication. Heat shock (50 °C, 1 h) also promoted shoot multiplication at these sucrose concentrations on both BM medium and BM supplemented with 5.0 µM BA. Beneficial effect of sucrose was also observed on rooting of shoots on BM as well as BM supplemented with 5.0 μM indole-3-butyric acid (IBA). Phytagel as a gelling agent was found to be more effective for shoot proliferation and growth compared to agar. Amongst various soil mixtures tested, higher survival of plants was observed in soil containing Vermicompost. It was interesting to note that a maximum plant survival (> 95 %) was observed when plants were directly transferred to net-house (irradiance reduced to 50 % with green net, without humidity and temperature control) than poly-house (with humidity and temperature control). Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) analysis of regenerated plants showed genetic similarity to mother plant.

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Section: Resultsmentioning

confidence: 99%

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Kumar¹,

Aggarwal²,

Gupta³

et al. 2010

Biologia plant.

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“…Among all the combinations, the best shoot multiplication was observed in BAP (4.43 μM) supplemented with 0.007% adenine sulphate, while the combination of NAA, IAA and IBA comparably resulted in the lowest multiplication. The superiority of BAP has been suggested over other cytokinins and auxins on medicinal plants (Dave et al 2003, Martin 2003. There are several reasons for preferred use of BAP and slow degradation may be the one so that it can be autoclaved easily.…”

Section: Resultsmentioning

confidence: 99%

Effects of Additives in Shoot Multiplication and Genetic Validation in Swertica chirayita Revealed through RAPD Analysis

Sharma¹,

Kamal²,

Dobriyal

et al. 2013

Plant Tissue Cult. & Biotech.

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Enhanced in vitro caulogenesis has been tested in Swertia chirayita on MS supplemented with BAP, IAA, IBA, NAA and additives like adenine sulfate and d-glutamine with 2.5% sucrose. The best in vitro caulogenesis was observed in MS fortified with 4.43 μM BAP in combination with IAA (0.8 μM) that resulted increase in shoot multiplication rate. The multiplication and elongation of shoots were further enhanced by the addition of adenine sulfate (0.007%) that resulted in the further increase in multiplication fold. The addition of adenine sulfate reduced the use of other cytokinins with different auxins reported in the previous studies on Swertia chirayita. The study suggests adenine sulfate as a primary assimilable reduced nitrogen source for enhancing the shoot multiplication and elongation in Swertia chirayita. RAPD markers were employed to check the genetic variation among the clonal stock that resulted in 97% similarity.

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“…Similar results were observed in Ormocarpum sennoides 24 , Adhathoda vasica 25 and Chlorophytum borivilianum 26 . The superiority of BAP has been suggested over other cytokinins and auxins on medicinal plants 6,[27][28] . The effects of higher concentration of cytokinins have been reported to reduce the number of shoots generated per explant 29,30 .…”

Section: Discussionmentioning

confidence: 99%

Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis

Sharma

Belwal²,

Kamal³

et al. 2016

Braz. arch. biol. technol.

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Scaling-up production and field performance of micropropagated medicinal herb ‘Safed Musli’ (Chlorophytum borivilianum)

Cited by 24 publications

References 10 publications

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Factors affecting in vitro propagation and field establishment of Chlorophytum borivilianum

Effects of Additives in Shoot Multiplication and Genetic Validation in Swertica chirayita Revealed through RAPD Analysis

Assessment of Genetic Fidelity of in vitro Raised Plants in Swertia chirayita through ISSR, RAPD analysis and Peroxidase Profiling during Organogenesis

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