Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives

Ragab, Tamer I.M.; Malek, Roslinda Abd; Elsehemy, Islam A.; Farag, Mohamed; Salama, Bassem M.; El-Baseer, Mohamed A. Abd; Gamal‐Eldeen, Amira M.; Enshasy, Hesham Ali El; Esawy, Mona A.

doi:10.1016/j.jbiosc.2018.09.008

Cited by 24 publications

(9 citation statements)

References 28 publications

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“…To investigate the biological activity of the purified P . fluorescens strain ES levan, cytotoxicity (SRB assay) was determined against A-431: Human epidermoid Skin carcinoma and HepG2: Hepatocellular carcinoma cell line that showed IC50 > 100 μg/ml, to assess the suitability of the polymer to be efficient in drug delivery applications since scientists are searching for causes that put the body at risk of cancer as well as factors that could protect the body from cancer ( Ragab et al, 2019 ). The results revealed that in the liver HepG2 cells, levan has moderate cytotoxicity with IC50 of 222.7 µg/ml, whereas lower cytotoxic effect indicated by elevated IC50 of 469 µg/ml was recorded against human epidermoid Skin carcinoma.…”

Section: Discussionmentioning

confidence: 99%

“…Differences in insensitivities of cell lines towards different anticancer substances could be due to their physiological behavior and structural characteristics ( Alqahtani et al, 2020 ). The previous study recorded the strong anti-cancer activity of levan from B. subtilis especially after sulfation, phosphorylation and carboxymethylation were done ( Esawy et al, 2013 , Ragab et al, 2019 ). Furthermore, bacterial levan was effective towards stomach cancer where its anticancer activity was increased proportionally to the complexity in the branching of levan structure ( Srikanth et al, 2015a , Srikanth et al, 2015b ).…”

Section: Discussionmentioning

confidence: 99%

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Partial characterization of levan polymer from Pseudomonas fluorescens with significant cytotoxic and antioxidant activity

Korany

El-Hendawy

Sonbol

et al. 2021

Saudi Journal of Biological Sciences

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Microbial levan has great potential as a functional biopolymer in different fields including foods, feeds, cosmetics, and the pharmaceutical and chemical industries. In this study, a good levan producer bacterial strain of Pseudomonas fluorescens strain ES, isolated from soil in Egypt in a previous study, was used. Levan production by this strain was optimized using Plackett-Burman experimental design (PBD) to screen the critical factors of several process variables and Centered Central Composite Design (CCD) was applied for further estimation of the relationship between the variables and the response as well as optimization of the levels. Plackett-Burman (P-B) design showed a p-value 0.0144 less than 0.05 indicated the significance of the model. Sucrose, potassium dihydrogen phosphate, yeast extract and pH value showed the most significant effect on levan concentration at the values of 89.17, 65.83, 24.17, and 15.83, respectively. The purified levan polymer was characterized using different Physico-chemical methods such as Fourier Transform Infrared Spectrometer (FTIR), Nuclear magnetic resonance (NMR), and High-Performance Liquid Chromatography (HPLC) to determine the main composition and functional groups in the obtained polymer. HPLC results indicated that the polymer purification increased the percentage of fructose residue from 75 up to 89. Furthermore, 1 H and 13 C NMR spectroscopy analysis showed great matching between the obtained signal for our polymer with that reported in other people ’ s work. The obtained levan polymer exhibited cytotoxic activity against Human epidermoid Skin carcinoma and Hepatocellular carcinoma with IC50 of 469 and 222.7 µg/ml, respectively. Antioxidant activity was determined using DPPH assay and the percentage of inhibition at 1000 µg/ml was found to be <50 (13.89 ± 1.07) with IC50 of (24.42 ± 0.87).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Partial characterization of levan polymer from Pseudomonas fluorescens with significant cytotoxic and antioxidant activity

Korany

El-Hendawy

Sonbol

et al. 2021

Saudi Journal of Biological Sciences

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“…In the spectrum of levan, the band at 3303 cm −1 was assigned to OH stretching vibration of the polysaccharide, while the bands at 2903 cm −1 and 2885 cm −1 were ascribed to C-H stretching vibration of fructose rings. The absorption bands appearing at 1123 cm −1 , 1012 cm −1 and 925 cm −1 , represent the C − O − C symmetric bending vibrations of fructofuranose rings and glycosidic linkages [42]. In the ZnPc spectrum, the characteristic bands over the region 430-1600 cm −1 were revealed.…”

Section: Characterization Of Lev-fe 3 O 4 and Lev-znpc-fe 3 O 4 Nanopmentioning

confidence: 95%

Photosensitizing potential of tailored magnetite hybrid nanoparticles functionalized with levan and zinc (II) phthalocyanine

Chełminiak-Dudkiewicz

Rybczyński

Smolarkiewicz-Wyczachowski

et al. 2020

Applied Surface Science

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Phototherapies, including photodynamic therapy (PDT), have been widely used in the treatment of various diseases, especially for cancer. However, there is still a lack of effective, safe photosensitizers that would be well tolerated by patients. The combination of several methods (like phototherapy and hyperthermia) constitutes a modern therapeutic approach, which demands new materials based on components that are non-toxic without irradiation. Therefore, this study presents the synthesis and properties of novel, advanced nanomaterials in which the advantage features of the magnetic nanoparticles and photoactive compounds were combined. The primary purpose of this work was the synthesis of magnetic nanoparticles coated with biocompatible and antitumor polysaccharide-levan, previously unknown from scientific literature, and the deposition of potent photosensitizerzinc(II) phthalocyanine on their surface. In order to better characterize the nature of the coating covering the magnetic core, the atomic force microscope analysis, a contact angle measurement, and the mechanical properties of pure levan and its blend with zinc(II) phthalocyanine films were investigated. This magnetic nanomaterial revealed the ability to generate singlet oxygen upon exposure to light. Finally, preliminary toxicity of obtained nanoparticles was tested using the Microtox® testwith and without irradiation.

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“…It will be interesting to compare sulfated LOS and flg22 in equimolar concentrations and compare the observed bursts. Sulfated levan already showed interesting effects in animal systems [79,126] and a direct induction of plant immunity has already been observed for other sulfated carbohydrates, such as algal ulvans, fucans and laminarins [127,128]. Generally, it will also be interesting to study fructans as direct elicitors on fructan-primed plants instead of unprimed ones, as this will give more insight into the effect of multiple fructan priming events, which should be considered for potential future applications in pest management.…”

Section: Discussionmentioning

confidence: 99%

Sweet Immunity Aspects during Levan Oligosaccharide-Mediated Priming in Rocket against Botrytis cinerea

Versluys

Ende

2022

Biomolecules

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New strategies are required for crop protection against biotic stress. Naturally derived molecules, including carbohydrates such as fructans, can be used in priming or defense stimulation. Rocket (Eruca sativa) is an important leafy vegetable and a good source of antioxidants. Here, we tested the efficacy of fructan-induced immunity in the Botrytis cinerea pathosystem. Different fructan types of plant and microbial origin were considered and changes in sugar dynamics were analyzed. Immune resistance increased significantly after priming with natural and sulfated levan oligosaccharides (LOS). No clear positive effects were observed for fructo-oligosaccharides (FOS), inulin or branched-type fructans. Only sulfated LOS induced a direct ROS burst, typical for elicitors, while LOS behaved as a genuine priming compound. Total leaf sugar levels increased significantly both after LOS priming and subsequent infection. Intriguingly, apoplastic sugar levels temporarily increased after LOS priming but not after infection. We followed LOS and small soluble sugar dynamics in the apoplast as a function of time and found a temporal peak in small soluble sugar levels. Although similar dynamics were also found with inulin-type FOS, increased Glc and FOS levels may benefit B. cinerea. During LOS priming, LOS- and/or Glc-dependent signaling may induce downstream sweet immunity responses.

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Scaling up of levan yield in Bacillus subtilis M and cytotoxicity study on levan and its derivatives

Cited by 24 publications

References 28 publications

Partial characterization of levan polymer from Pseudomonas fluorescens with significant cytotoxic and antioxidant activity

Partial characterization of levan polymer from Pseudomonas fluorescens with significant cytotoxic and antioxidant activity

Photosensitizing potential of tailored magnetite hybrid nanoparticles functionalized with levan and zinc (II) phthalocyanine

Sweet Immunity Aspects during Levan Oligosaccharide-Mediated Priming in Rocket against Botrytis cinerea

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