Scale‐Up Preparation of Manganese‐Iron Prussian Blue Nanozymes as Potent Oral Nanomedicines for Acute Ulcerative Colitis

Abstract

Prussian blue (PB) nanozymes have been demonstrated as effective therapeutics for ulcerative colitis (UC), yet an unmet practical challenge remains in the scalable production of these nanozymes and uncertainty over their efficacy. With a novel approach, we prepare a series of porous manganese‐iron PB (MnPB) colloids, which have been shown to be efficient scavengers for reactive oxygen species (ROS) including hydroxyl radical, superoxide anion, and hydrogen peroxide. In vitro cellular experiments confirm the capability of the nanozyme to protect cells from ROS attack. In vivo, the administration of MnPB nanozyme through gavage at a dosage of 10 mg k−1g/day for three doses in total potently ameliorates the pathological symptoms of acute UC in a murine model, resulting in mitigated inflammatory responses and improved viability rate. Significantly, the nanozyme produced at a large scale can be achieved at an unprecedented yield weighting approximately 11 g per batch of reaction, demonstrating comparable anti‐ROS activities and treatment efficacy to its small‐scale counterpart. This work represents the first demonstration of the scale‐up preparation of PB analogue nanozymes for UC without compromising treatment efficacy, laying the foundation for further testing of these nanozymes on larger animals and promising clinical translation.This article is protected by copyright. All rights reserved

Dental Caries Management with Antibacterial Silver-Doped Prussian Blue Hydrogel by the Combined Effects of Photothermal Response and Ion Discharge

“…The mechanism by which the AgPB nanoparticles are formed can be explained by the air-assisted oxidation of Fe­(CN) 6 4– to Fe­(CN) 6 3– , which then combine with Fe 2+ ions from the dissociation of Fe­(CN) 6 4– ions to generate PB, during the process the doping events of Ag + and K + ions occur in the interstitial sites of PB. By applying this strategy, we recently reported the preparation of a series of novel structured Mn-doped PB colloids . Compared to the conventional use of Fe­(CN) 6 3– ions as the precursor for preparing PB nanoparticles, the use of Fe­(CN) 6 4– ions is featured by a lower reaction temperature, reductant free, and a high prospect for large-scale preparation.…”