Scale-up of recombinant hirudin production fromSaccharomyces cerevisiae

Kim, Chul Ho; Rao, K. Jagannadha; Youn, Duk Joong; Rhee, Shin Hyung

doi:10.1007/bf02949222

Cited by 15 publications

(8 citation statements)

References 7 publications

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“…4B). In contrast to the results reported by Castan [6], both the yield coefficients of r-hGH and biomass did not vary much for either the air supply or the high purity oxygen supply, as shown in Tab. 2.…”

Section: Effects Of Oxygen Supplycontrasting

confidence: 83%

Effects of Oxygen Supply Modes on the Production of Human Growth Hormone in Different Scale Bioreactors

Shang¹,

Tian²,

Kim³

et al. 2009

Chem Eng & Technol

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Recombinant Escherichia coli has been studied as a main host for recombinant protein productions, but it is still difficult to cultivate E. coli in a large industrialscale process due to the oxygen supply limitation. In this study, E. coli BL(21) harboring a new constructed plasmid (pEHUb-hGH) was used for producing recombinant human growth hormone (r-hGH) in 5-L and 30-L scale fermentors by supplying air and high purity oxygen, respectively, where the high purity oxygen was produced from a vacuum pressure swing adsorption (PSA). The impact of oxygen supply modes, i.e., air and high purity oxygen, on cell growth and r-hGH production was investigated in different scale fermentors. In the case of high purity oxygen supply, the final cell density and r-hGH concentrations were 63.0 and 4.8 g/L in the 5-L fermentor, 51.6 and 4.0 g/L in the 30-L fermentor, respectively. In addition, the productivity of r-hGH was doubled in the 5-L fermentor, and increased 4-fold in the 30-L fermentor, compared to the results obtained in the case of the air supply. The supply of high purity oxygen eliminated the oxygen limitation and acetate formation effectively, and apparently, did not affect the degradation of r-hGH. This shows that the recombinant E. coli cultivation with high purity oxygen produced from PSA may provide an effective method for large-scale industrial production of recombinant proteins.

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Section: Effects Of Oxygen Supplycontrasting

confidence: 83%

Effects of Oxygen Supply Modes on the Production of Human Growth Hormone in Different Scale Bioreactors

Shang¹,

Tian²,

Kim³

et al. 2009

Chem Eng & Technol

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“…The high importance of oxygen for microorganisms with a high oxygen demand like Corynebacterium glutamicum makes a constant volumetric mass transfer coefficient (k L a) an efficient scale-up strategy. The strategy was successfully applied based on experimental data for batch cultures by Gupta and Rao (2003), and by Kim et al (2003) for the scale-up from laboratory to industrial fermenters. However, it has not yet been applied based on empirical correlations or for continuous cultures.…”

Section: Introductionmentioning

confidence: 99%

Scale‐up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Seletzky

Noak

Fricke

et al. 2007

Biotech & Bioengineering

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Scale-up from shake flasks to fermenters has been hampered by the lack of knowledge concerning the influence of operating conditions on mass transfer, hydromechanics, and power input. However, in recent years the properties of shake flasks have been described with empirical models. A practical scale-up strategy for everyday use is introduced for the scale-up of aerobic cultures from shake flasks to fermenters in batch and continuous mode. The strategy is based on empirical correlations of the volumetric mass transfer coefficient (k(L) a) and the pH. The accuracy of the empirical k(L) a correlations and the assumptions required to use these correlations for an arbitrary biological medium are discussed. To determine the optimal pH of the culture medium a simple laboratory method based on titration curves of the medium and a mechanistic pH model, which is solely based on the medium composition, is applied. The effectiveness of the scale-up strategy is demonstrated by comparing the behavior of Corynebacterium glutamicum on lactic acid in shake flasks and fermenters in batch and continuous mode. The maximum growth rate (micro(max) = 0.32 h(-1)) and the oxygen substrate coefficient (Y O2 /S= 0.0174 mol/l) of C. glutamicum on lactic acid were equal for shake flask, fermenter, batch, and continuous cultures. The biomass substrate yield was independent of the scale, but was lower in batch cultures (Y(X/S) = 0.36 g/g) than in continuous cultures (Y(X/S) = 0.45 g/g). The experimental data (biomass, respiration, pH) could be described with a simple biological model combined with a mechanistic pH model.

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“…Furthermore, k L a and Pg V L have been reported as the most applied and successful scale-up parameters in aerobic processes, (Aiba et al, 1973;Perry Handbook, 2001;Wong et al, 2002Wong et al, , 2003Kim et al, 2003;Betts et al, 2006), where oxygen transfer is the limiting factor. …”

Section: Vesselmentioning

confidence: 98%

Scale up and design of processes in aerated-stirred fermenters for the industrial production of vinegar

González-Sáiz

Garrido-Vidal

Pizarro

2009

Journal of Food Engineering

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Scale-up of recombinant hirudin production fromSaccharomyces cerevisiae

Cited by 15 publications

References 7 publications

Effects of Oxygen Supply Modes on the Production of Human Growth Hormone in Different Scale Bioreactors

Effects of Oxygen Supply Modes on the Production of Human Growth Hormone in Different Scale Bioreactors

Scale‐up from shake flasks to fermenters in batch and continuous mode with Corynebacterium glutamicum on lactic acid based on oxygen transfer and pH

Scale up and design of processes in aerated-stirred fermenters for the industrial production of vinegar

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