Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation

Abstract

Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate.

Optimization of Fermentation Medium for Enhanced Glucansucrase and Glucan Production from Weissella confusa

“…Dols et al, 1996 reported effect of added glucose, fructose and mannitol on dextransucrase and dextran yield from Leuconostoc mesenteroides NRRL B-1299. The aeration conditions were optimized for production and scale up of dextransucrase from Leuconostoc mesenteroides in a bioreactor (Michelena et al, 2003). In our earlier study high glucan production ability of Weissella confusa (Cab3) isolated from fermented cabbage was explored and compared with other lactic acid bacteria (Shukla and Goyal, 2011).…”

Production and Characterization of Dextran Biosynthesizing Glucosyltransferase From L. Meseneteroides Kibge-Ib40

“…In another study, L. mesenteroides AA1 also produces maximum dextransucrase at 08 hours of fermentation period (Aman et al, 2012). Dextransucrase from L. mesenteroides B/110-1-1 achieved maximum growth rate and enzyme titer between 6 to 7 hours of fermentation (Michelena et al, 2003). Optimum temperature was also observed by incubating the microbes at different temperatures (20°C, 25°C and 30°C).…”

Oxygen transfer rate modulates the dextransucrase production by Acetobacter tropicalis

“…The values of K L a for 1.0 vvm at 300 rpm, 0.5 vvm at 450 rpm and 1.0 vvm at 600 rpm were 0.18 min -1 , 0.28 min -1 and 0.29 min -1 respectively. The fermentation kinetics of Leuconostoc mesenteroides in a 5 L bioreactor for the production of dextransucrase was studied and K L a obtained was 30.85 h -1 at 0.15 vvm aeration rate and 225 rpm [31]. In case of ß-glucuronidase production by recombinant E. coli, highest enzyme production was obtained at 300 rpm agitation and 2 vvm aeration having K L a value 1.686 h -1 [32].…”