Scale-up cultivation enhanced arachidonic acid accumulation by red microalgae Porphyridium purpureum

Chang, Jingyu; Le, Kai; Song, Xiaoqiang; Jiao, Kailin; Zeng, Xianhai; Ling, Xueping; Shi, Tianshu; Tang, Xing; Sun, Yong

doi:10.1007/s00449-017-1831-x

Cited by 14 publications

(3 citation statements)

References 31 publications

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“…Porphyridium purpureum has the ability to grow in different culture systems and locations [14], coupled with the capacity to produce and accumulate a large amount of PE, which production can be enhanced when the microalgae are cultured under stress conditions [11]. The effect of different physical and nutritional factors on the culture conditions of different microalgae groups has been investigated for several purposes, including the production of biomass, pigments, oil, and other metabolites [11,15,16,17,18]. Light intensity is one of the most critical factors that influence microalgal pigment composition.…”

Section: Introductionmentioning

confidence: 99%

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

et al. 2019

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Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity.

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Section: Introductionmentioning

confidence: 99%

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

et al. 2019

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“…The concentration of PUFAs generally decreases at increasing temperatures. For example, EPA accumulation declined markedly with the increase of temperature also in the red alga Porphirium purpureum [38]. A significant decrease of EPA, DHA, and other PUFAs with increasing temperature (from 15 to 25 • C) was also observed in the diatom Phaeodactylum tricornutum [39].…”

Section: Temperaturementioning

confidence: 84%

Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture

et al. 2021

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Microalgae have a great potential for the production of healthy food and feed supplements. Their ability to convert carbon into high-value compounds and to be cultured in large scale without interfering with crop cultivation makes these photosynthetic microorganisms promising for the sustainable production of lipids. In particular, microalgae represent an alternative source of polyunsaturated fatty acids (PUFAs), whose consumption is related to various health benefits for humans and animals. In recent years, several strategies to improve PUFAs’ production in microalgae have been investigated. Such strategies include selecting the best performing species and strains and the optimization of culturing conditions, with special emphasis on the different cultivation systems and the effect of different abiotic factors on PUFAs’ accumulation in microalgae. Moreover, developments and results obtained through the most modern genetic and metabolic engineering techniques are described, focusing on the strategies that lead to an increased lipid production or an altered PUFAs’ profile. Additionally, we provide an overview of biotechnological applications of PUFAs derived from microalgae as safe and sustainable organisms, such as aquafeed and food ingredients, and of the main techniques (and their related issues) for PUFAs’ extraction and purification from microalgal biomass.

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“…For the control sample (L0), the continuous illumination system aids in increasing the percentage of polyunsaturated fatty acids in the lipid fraction: C18:0 decreases, and C18:1 and C18:2 concentration increases. The shift in fatty acid composition could be a result of the stress factors present during microalgae growth, specifically light stress, which favors the metabolic pathway resulting in the transformation of C18:0 to polyunsaturated fatty acids [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Nannochloris sp. Microalgae Strain for Treatment of Dairy Wastewaters

et al. 2023

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This paper focuses on a process for dairy wastewater treatment by mixotrophic cultivation of microalgae Nannochloris sp., using cheese whey obtained as a side flow from cheese production as an organic carbon source. The microalgae samples were prepared by adding to the standard growth medium increasing amounts of cheese whey, calculated to ensure a lactose concentration between 0 and 10 g/L. The samples were incubated at a constant temperature of 28 °C and 175 rpm stirring speed for a total time of seven days. Two LED (Light Emitting Diode) illumination schemes were applied in order to assess the effect of this parameter on microalgae development and bioactive compound accumulation: continuous illumination (light stress) versus alternative cycles of 12 h light—12 h dark (day–night cycle). The growth medium was analyzed before and after microalgae cultivation in order to determine the reduction of carbon, nitrogen, and phosphorus. The results obtained for this process, after a seven-day cultivation period, were as follows: reduction of 99–100% of lactose from the growth medium, up to 96% reduction in chemical oxygen demand, up to 91% reduction in nitrogen content, and up to 70% reduction in phosphorus content.

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Scale-up cultivation enhanced arachidonic acid accumulation by red microalgae Porphyridium purpureum

Cited by 14 publications

References 31 publications

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture

Nannochloris sp. Microalgae Strain for Treatment of Dairy Wastewaters

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