Scale-up and fed-batch cultivation strategy for the enhanced co-production of microbial lipids and carotenoids using renewable waste feedstock

Villegas-Méndez, Miguel Ángel; Montañez, Julio; Contreras-Esquivel, Juan Carlos; Salmerón, Iván; Koutinas, Apostolis; Morales‐Oyervides, Lourdes

doi:10.1016/j.jenvman.2023.117866

Cited by 9 publications

(5 citation statements)

References 55 publications

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“…Specifically, it achieved biomass yields of 89.84 ± 0.41 g/L with glucose and 87.78 ± 0.30 g/L with sucrose, and β-carotene yields of 2.80 ± 0.02 mg/g cell with glucose and 3.25 ± 0.03 mg/g cell with sucrose. The productivity rates were also remarkable higher than that reported in all literature reviewed 47 – 52 in Table 3 . The biomass production rates ( Q x ) of 0.94 ± 0.00 g/L.h for glucose and 0.91 ± 0.01 g/L.h for sucrose, and β-carotene production rates ( Q p ) of 2.62 ± 0.01 mg/L.h for glucose and 2.97 ± 0.02 mg/L.h for sucrose were achived in this present work.…”

Section: Resultscontrasting

confidence: 58%

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Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Thumkasem,

On-mee,

Kongsinkaew

et al. 2024

Sci Rep

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This study assessed Rhodotorula paludigena CM33's growth and β-carotene production in a 22-L bioreactor for potential use as an aquatic animal feed supplement. Optimizing the feed medium's micronutrient concentration for high-cell-density fed-batch cultivation using glucose as the carbon source yielded biomass of 89.84 g/L and β-carotene concentration of 251.64 mg/L. Notably, using sucrose as the carbon source in feed medium outperforms glucose feeds, resulting in a β-carotene concentration of 285.00 mg/L with a similar biomass of 87.78 g/L. In the fed-batch fermentation using Sucrose Feed Medium, R. paludigena CM33 exhibited high biomass production rates (Qx) of 0.91 g/L.h and remarkable β-carotene production rates (Qp) of 2.97 mg/L.h. In vitro digestibility assays showed that R. paludigena CM33, especially when cultivated using sucrose, enhances protein digestibility affirming its suitability as an aquatic feed supplement. Furthermore, R. paludigena CM33's nutrient-rich profile and probiotic potential make it an attractive option for aquatic nutrition. This research highlights the importance of cost-effective carbon sources in large-scale β-carotene production for aquatic animal nutrition.

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Section: Resultscontrasting

confidence: 58%

“…Table 3 presents a comparative analysis with other literature reviews focusing on fed-batch cultivation 47 – 52 . Among these, R. paludigena CM33 stands out for its superior β-carotene production, particularly notable when employing a DOT stat feed control strategy.…”

Section: Resultsmentioning

confidence: 99%

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Thumkasem,

On-mee,

Kongsinkaew

et al. 2024

Sci Rep

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“…The fed-batch strategy is commonly used in microbial fermentation to avoid substrate inhibition and achieve high cell/product concentrations under controlled dynamics of the addition of concentrated substrate/s solution, such as in the production of bioethanol, microbial lipids, lactic acid, etc. [ 88 , 89 , 90 ]. Initial nutrient concentration in the medium was equal to the optimal ratio Si:N:P = 7:23:1 mol:mol:mol.…”

Section: Resultsmentioning

confidence: 99%

Enhancement of Biomass Production of Diatom Nitzschia sp. S5 through Optimisation of Growth Medium Composition and Fed-Batch Cultivation

Grubišić,

Šantek,

Kuzmić

et al. 2024

Marine Drugs

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The growing commercial application of microalgae in different industry sectors, including the production of bioenergy, pharmaceuticals, nutraceuticals, chemicals, feed, and food, demands large quantities of microalgal biomass with specific compositions produced at reasonable prices. Extensive studies have been carried out on the design of new and improvement of current cultivation systems and the optimisation of growth medium composition for high productivity of microalgal biomass. In this study, the concentrations of the main macronutrients, silicon, nitrogen and phosphorus, essential for the growth of diatom Nitzschia sp. S5 were optimised to obtain a high biomass concentration. The effect of main macronutrients on growth kinetics and cell composition was also studied. Silicon had the most significant effect on diatom growth during batch cultivation. The concentration of biomass increased 5.45-fold (0.49 g L−1) at 1 mM silicon concentration in modified growth medium compared to the original Guillard f/2 medium. Optimisation of silicon, nitrogen, and phosphorus quantities and ratios further increased biomass concentration. The molar ratio of Si:N:P = 7:23:1 mol:mol:mol yielded the highest biomass concentration of 0.73 g L−1. Finally, the fed-batch diatom cultivation of diatom using an optimised Guillard f/2 growth medium with four additions of concentrated macronutrient solution resulted in 1.63 g L−1 of microalgal biomass. The proteins were the most abundant macromolecules in microalgal biomass, with a lower content of carbohydrates and lipids under all studied conditions.

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“…The sugars were released from wastes after enzymatic treatment using Aspergillus luchuensis . After centrifugation and filtration, the substrate was adjusted to pH 5.0, supplemented with selected salts and successfully used for simultaneous lipids and carotenoid production (Villegas-Méndez et al 2023 ). A highly demanded product in medicine, cosmetology, and pharmacy is bacterial cellulose membrane.…”

Section: Exploiting Low Ph In Waste Management and In The Revalorizat...mentioning

confidence: 99%

Exploitation of microbial activities at low pH to enhance planetary health

Atasoy,

Álvarez Ordóñez,

Cenian

et al. 2023

FEMS Microbiology Reviews

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Awareness is growing that human health cannot be considered in isolation but is inextricably woven with the health of the environment in which we live. It is however under-recognised that the sustainability of human activities strongly relies on preserving the equilibrium of the microbial communities living in/on/around us. Microbial metabolic activities are instrumental for production, functionalization, processing and preservation of food. For circular economy, microbial metabolism would be exploited to produce building blocks for the chemical industry, to achieve effective crop protection, agri-food waste revalorization or biofuel production, as well as in bioremediation and bioaugmentation of contaminated areas. Low pH is undoubtedly a key physical-chemical parameter that needs to be considered for exploiting the powerful microbial metabolic arsenal. Deviation from optimal pH conditions has profound effects on shaping the microbial communities responsible for carrying out essential processes. Furthermore, novel strategies to combat contaminations and infections by pathogens rely on microbial-derived acidic molecules that suppress/inhibit their growth. Herein, we present the state-of-the-art of the knowledge on the impact of acidic pH in many applied areas and how this knowledge can guide us to use the immense arsenal of microbial metabolic activities for their more impactful exploitation in a Planetary Health perspective.

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Scale-up and fed-batch cultivation strategy for the enhanced co-production of microbial lipids and carotenoids using renewable waste feedstock

Cited by 9 publications

References 55 publications

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Enhanced high β-carotene yeast cell production by Rhodotorula paludigena CM33 and in vitro digestibility in aquatic animals

Enhancement of Biomass Production of Diatom Nitzschia sp. S5 through Optimisation of Growth Medium Composition and Fed-Batch Cultivation

Exploitation of microbial activities at low pH to enhance planetary health

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