Scalable Synthesis of Biodegradable Black Mesoporous Silicon Nanoparticles for Highly Efficient Photothermal Therapy

Xu, Wujun; Tamarov, Konstantin; Fan, Li; Granroth, Sari; Rantanen, Jimi; Nissinen, Tuomo; Peräniemi, Sirpa; Uski, Oskari; Hirvonen, Maija-Riitta; Lehto, Vesa‐Pekka

doi:10.1021/acsami.8b04557

Cited by 39 publications

(46 citation statements)

References 43 publications

(73 reference statements)

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“…We firstly detected differential gene expression when cells fed with the dual PEG functional black porous silica nanoparticles (BPSi NPs) (offered by our collaborating lab [12]). The change of the zeta potential from − 18.5 to + 2.8 mV also indicated that the surface dual-PEGylation was successful (Fig.…”

Section: Resultsmentioning

confidence: 99%

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The Establishment and Application Studies on Precise Lysosome pH Indicator Based on Self-Decomposable Nanoparticles

Pang

Song

et al. 2020

Nanoscale Res Lett

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Acidic pH of lysosomes is closely related to autophagy; thus, well known of the precise lysosomes, pH changes will give more information on the autophagy process and status. So far, however, only pH changes in a relatively broad range could be indicated, the exact lysosomes pH detection has never arrived. In our study, we established an endo/ lysosome pH indicator based on the self-decomposable SiO 2 nanoparticle system with specific synthesis parameters. The central concentrated methylene blue (MB) in the central-hollow structural nanoparticles presented sensitive release as a function of pH values from pH 4.0-4.8, which is exactly the pH range of lysosomes. The linear correlation of the optical density (OD) values and the pH values has been built up, which has been used for the detection of lysosomes pH in 6 different cell lines. Moreover, by this system, we succeeded in precisely detecting the pH average changes of lysosomes before and after black mesoporous silicon (BPSi) NP endocytosis, clarifying the mechanism of the autophagy termination after BPSi endocytosis. So, the self-decomposable nanoparticle-based luminal pH indicator may provide a new methodology and strategy to know better of the lysosome pH, then indicate more details on the autophagy process or other important signaling about metabolisms.

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Section: Resultsmentioning

confidence: 99%

“…The BPSi nanoparticles were prepared by our previous method [12] and supplied by our collaborator (Wujun Xu, Department of Applied Physics, University of Eastern Finland). The preparation of BPSi, NaSi, ammonium salt, and NaBr (NaSi: NH 4 Br: NaBr of 1:4:4, w/w/w) was ground in a glove box with an Ar atmosphere.…”

Section: Bpsi Nanoparticle Synthesismentioning

confidence: 99%

The Establishment and Application Studies on Precise Lysosome pH Indicator Based on Self-Decomposable Nanoparticles

Pang

Song

et al. 2020

Nanoscale Res Lett

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“…We firstly detected differential gene expression when cells fed with the dual PEG functional black porous silica nanoparticles (BPSi NPs) (offered by our collaborating lab [11]). Based on the Cluster heat map of differential gene expression ( Figure S1), we selected more than 2 fold differential gene expressions for further investigation.…”

Section: Resultsmentioning

confidence: 99%

“…The BPSi nanoparticls were prepared by our previous method [11], and supplied by our collaborator (Wujun Xu, Department of Applied Physics, University of Eastern Finland).…”

Section: Bpsi Nanoparticles Synthesismentioning

confidence: 99%

Precise lysosome pH indicator based on self-decomposable nanoparticles

Pang

Song

et al. 2020

Preprint

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Background Acidic pH of lysosomes are closely related to the autophagy, thus well known of the precise lysosomes pH changes will give more information on the autophagy process and status. So far, however, only pH changes in a relative broad range could be indicated, the exactly lysosomes pH detection has never arrived. Methods In our study, we established an endo/lysosome pH indicator based on self-decomposable SiO 2 nanoparticle system with specific synthesis parameters. The central concentrated MB in the central-hollow structural nanoparticles presented sensitive release as a function of pH values from pH 4.0-4.8, which is the exactly the pH range of lysosomes. The linear correlation of the OD values and the pH values has been built up, which has been used for detection of lysosomes pH in 6 different cell lines. Results By this system, we succeeded in precise detecting the pH average changes of lysosomes before and after BPSi NPs endocytosis, clarifying the mechanism of the autophagy termination after BPSi endocytosis. Conclusions The self-decomposable nanoparticles based luminal pH indicator may provide a new methodology and strategy to know better of the lysosome pH, then indicate more details on autophagy process or other important signaling about metabolism.

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“…In our previous study, 22 a new type of black mesoporous silicon (BPSi) nanoparticles was developed via the chemical oxidation method. The BPSi nanoparticles possessed better photothermal performance than gold nanoparticles, carbon nanotubes, and Fe 3 O 4 , 22 which have widely been studied as contrast agents in photoacoustic imaging. 10 , 12 , 23 Therefore, due to their outstanding photothermal performance, we hypothesize that the BPSi nanoparticles can serve as novel contrast agents to enhance the imaging sensitivity of PAT.…”

Section: Introductionmentioning

confidence: 99%

Black Mesoporous Silicon as a Contrast Agent for LED-Based 3D Photoacoustic Tomography

Leskinen

Tick

et al. 2020

ACS Appl. Mater. Interfaces

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Mesoporous silicon (PSi) nanoparticles have been widely studied in different biomedical imaging modalities due to their several beneficial material properties. However, they have not been found to be suitable for photoacoustic imaging due to their poor photothermal conversion performance. In the present study, biodegradable black mesoporous silicon (BPSi) nanoparticles with strong light absorbance were developed as superior image contrast agents for photoacoustic tomography (PAT), which was realized with a light-emitting diode (LED) instead of the commonly used laser. LED-based PAT offers the advantages of low cost, compactness, good mobility, and easy operation as compared to the traditional laser-based PAT modality. Nevertheless, the poor imaging sensitivity of the LED-PAT systems has been the main barrier to prevent their wide biomedical application because the LED light has low optical energy. The present study demonstrated that the imaging sensitivity of the LED-PAT system was significantly enhanced with the PEGylated BPSi (PEG–BPSi) nanoparticles. The PEG–BPSi nanoparticles were clearly detectable with a low concentration of 0.05 mg/mL in vitro and with an LED radiation energy of 5.2 μJ. The required concentration of the PEG–BPSi nanoparticles was 10 times lesser than that of the reference gold nanoparticles to reach the corresponding level of the imaging contrast. The ex vivo studies demonstrated that the submillimeter BPSi nanoparticle-based absorbers were distinguishable in chicken breast tissues. The strong contrast provided by the BPSi particles indicated that these particles can be utilized as novel contrast agents in PAT, especially in LED-based systems with low light intensity.

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Scalable Synthesis of Biodegradable Black Mesoporous Silicon Nanoparticles for Highly Efficient Photothermal Therapy

Cited by 39 publications

References 43 publications

The Establishment and Application Studies on Precise Lysosome pH Indicator Based on Self-Decomposable Nanoparticles

The Establishment and Application Studies on Precise Lysosome pH Indicator Based on Self-Decomposable Nanoparticles

Precise lysosome pH indicator based on self-decomposable nanoparticles

Black Mesoporous Silicon as a Contrast Agent for LED-Based 3D Photoacoustic Tomography

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