Scalable mesenchymal stem cell enrichment from bone marrow aspirate using deterministic lateral displacement (DLD) microfluidic sorting

Tan Kwan Zen, Nicholas; Zeming, Kerwin Kwek; Teo, Kim Leng; Loberas, Mavis; Lee, Jialing; Goh, Chin Ren; Yang, Da Hou; Oh, Steve; Hui Hoi Po, James; Cool, Simon M.; Hou, Han Wei; Han, Jongyoon

doi:10.1039/d3lc00379e

Cited by 7 publications

(1 citation statement)

References 65 publications

(87 reference statements)

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“…Selection of specific cells from complex biological samples using microfluidic technologies has been intensively studied over the past decades, and many new principles for continuous cell sorting have been devised. − The sorting targets of biological samples subjected to microfluidics are not limited to cells but vary from submicrometer-sized extracellular vesicles to large cell clusters or living organisms. Representative applications of microfluidic cell sorters include the detection of rare cells as disease markers, , sorting of highly potent stem cells, , and subpopulation characterization of specific cell populations . In particular, the isolation of circulating tumor cells (CTCs) from blood samples assisted by microengineered structures has proven to be significantly useful for early tumor diagnosis and evaluation of antitumor drug efficacy. , Various physicochemical properties of cells have been utilized for sorting, including size, density, deformability, and surface markers.…”

Section: Introductionmentioning

confidence: 99%

High-Density Microporous Drainage-Integrating Sheath Flow Generator for Streamlining Microfluidic Cell Sorting Systems

Hayashi,

Hemmi,

Saito

et al. 2024

Anal. Chem.

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Tremendous efforts have been made to develop practical and efficient microfluidic cell and particle sorting systems; however, there are technological limitations in terms of system complexity and low operability. Here, we propose a sheath flow generator that can dramatically simplify operational procedures and enhance the usability of microfluidic cell sorters. The device utilizes an embedded polydimethylsiloxane (PDMS) sponge with interconnected micropores, which is in direct contact with microchannels and seamlessly integrated into the microfluidic platform. The high-density micropores on the sponge surface facilitated fluid drainage, and the drained fluid was used as the sheath flow for downstream cell sorting processes. To fabricate the integrated device, a new process for sponge-embedded substrates was developed through the accumulation, incorporation, and dissolution of PMMA microparticles as sacrificial porogens. The effects of the microchannel geometry and flow velocity on the sheath flow generation were investigated. Furthermore, an asymmetric lattice-shaped microchannel network for cell/particle sorting was connected to the sheath flow generator in series, and the sorting performances of model particles, blood cells, and spiked tumor cells were investigated. The sheath flow generation technique developed in this study is expected to streamline conventional microfluidic cell-sorting systems as it dramatically improves versatility and operability.

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