Scalable culture of human pluripotent stem cells in 3D

McDevitt, Todd C.

doi:10.1073/pnas.1320575111

Cited by 18 publications

(13 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to 2D monolayers, 3D culture systems offer the benefits of native cell-cell and cell-matrix interactions that improve the efficiency of spatial-temporal signals [249] [250], essential for cell proliferation and functioning [251]. Additionally, 3D culture platforms augment the space available for cell proliferating, thus enabling a scaling up of iPSC expansion without triggering the formation of unfavorable clusters and yielding higher cell densities and larger spheroids than 2D systems [252]. Expansion of iPSCs has been proven to be highly efficient in 3D scalable, synthetic platforms [225].…”

Section: Application Of 3d Cell Technologymentioning

confidence: 99%

Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications

Liu

David

Trawczynski

et al. 2019

Stem Cell Rev and Rep

353

209

View full text Add to dashboard Cite

Over the past 20 years, and particularly in the last decade, significant developmental milestones have driven basic, translational, and clinical advances in the field of stem cell and regenerative medicine. In this article, we provide a systemic overview of the major recent discoveries in this exciting and rapidly developing field. We begin by discussing experimental advances in the generation and differentiation of pluripotent stem cells (PSCs), next moving to the maintenance of stem cells in different culture types, and finishing with a discussion of three-dimensional (3D) cell technology and future stem cell applications. Specifically, we highlight the following crucial domains: 1) sources of pluripotent cells; 2) next-generation in vivo direct reprogramming technology; 3) cell types derived from PSCs and the influence of genetic memory; 4) induction of pluripotency with genomic modifications; 5) construction of vectors with reprogramming factor combinations; 6) enhancing pluripotency with small molecules and genetic signaling pathways; 7) induction of cell reprogramming by RNA signaling; 8) induction and enhancement of pluripotency with chemicals; 9) maintenance of pluripotency and genomic stability in induced pluripotent stem cells (iPSCs); 10) feeder-free and xenon-free culture environments; 11) biomaterial applications in stem cell biology; 12) three-dimensional (3D) cell technology; 13) 3D bioprinting; 14) downstream stem cell applications; and 15) current ethical issues in stem cell and regenerative medicine. This review, encompassing the fundamental concepts of regenerative medicine, is intended to provide a comprehensive portrait of important progress in stem cell research and development. Innovative technologies and real-world applications are emphasized for readers interested in the exciting, promising, and challenging field of stem cells and those seeking guidance in planning future research direction.

show abstract

Section: Application Of 3d Cell Technologymentioning

confidence: 99%

Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications

Liu

David

Trawczynski

et al. 2019

Stem Cell Rev and Rep

353

209

View full text Add to dashboard Cite

show abstract

“…This hydrogel enables simple encapsulation and retrieval of hPSCs by going from liquid to a solid gel with temperature switches between 4°C and 37°C. This specific hydrogel matrix also demonstrates significantly increased cell expansion from 2D adherent formats, as well as a more homogenous population that retains its pluripotent phenotype (McDevitt, 2013), making it a scalable and compatible platform for manufacturing practices.…”

Section: Organoids: Self-organizing Systems Of Stem Cells and Their Pmentioning

confidence: 99%

Engineering Stem Cell Organoids

et al. 2016

View full text Add to dashboard Cite

Organoid systems leverage the self-organizing properties of stem cells to create diverse multi-cellular tissue proxies. Most organoid models only represent single or partial components of a tissue, and it is often difficult to control the cell type, organization, and cell-cell/cell-matrix interactions within these systems. Herein, we discuss basic approaches to generate stem cell-based organoids, their advantages and limitations, and how bioengineering strategies can be used to steer the cell composition and their 3D organization within organoids to further enhance their utility in research and therapies.

show abstract

“…To address the challenge of hPSC expansion, Schaffer and coworkers developed a fully defined and scalable 4D culture system using a non-covalent thermoreversible hydrogel, poly( N -isopropylacrylamide)-co-poly(ethylene glycol) (PNIPPAAm-PEG) [57,74]. These hydrogels dissolved at lower temperatures, when the polymer network becomes more hydrophilic, and reformed at high temperatures to encapsulate, passage, and expand stem cells.…”

Section: 4d Biomaterials For Stem Cell Culturesmentioning

confidence: 99%

Biomaterials for 4D stem cell culture

Hilderbrand

Ovadia

Rehmann

et al. 2016

Current Opinion in Solid State and Materials Science

View full text Add to dashboard Cite

Stem cells reside in complex three-dimensional (3D) environments within the body that change with time, promoting various cellular functions and processes such as migration and differentiation. These complex changes in the surrounding environment dictate cell fate yet, until recently, have been challenging to mimic within cell culture systems. Hydrogel-based biomaterials are well suited to mimic aspects of these in vivo environments, owing to their high water content, soft tissue-like elasticity, and often-tunable biochemical content. Further, hydrogels can be engineered to achieve changes in matrix properties over time to better mimic dynamic native microenvironments for probing and directing stem cell function and fate. This review will focus on techniques to form hydrogel-based biomaterials and modify their properties in time during cell culture using select addition reactions, cleavage reactions, or non-covalent interactions. Recent applications of these techniques for the culture of stem cells in four dimensions (i.e., in three dimensions with changes over time) also will be discussed for studying essential stem cell processes.

show abstract

Scalable culture of human pluripotent stem cells in 3D

Cited by 18 publications

References 20 publications

Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications

Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications

Engineering Stem Cell Organoids

Biomaterials for 4D stem cell culture

Contact Info

Product

Resources

About