2015
DOI: 10.3791/52755
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Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System

Abstract: Continued advancement in pluripotent stem cell culture is closing the gap between bench and bedside for using these cells in regenerative medicine, drug discovery and safety testing. In order to produce stem cell derived biopharmaceutics and cells for tissue engineering and transplantation, a cost-effective cell-manufacturing technology is essential. Maintenance of pluripotency and stable performance of cells in downstream applications (e.g., cell differentiation) over time is paramount to large scale cell pro… Show more

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Cited by 18 publications
(20 citation statements)
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“…Furthermore, a lack of standardization in methodology between laboratories likely introduces further variability 9 . While previous work has automated the expansion of individual lines [10][11][12][13] , we reasoned that developing a fully automated, modular platform for parallel iPSC derivation, expansion and differentiation would allow us to identify, and minimize, factors contributing to variability in iPSC behavior as well as provide a platform for large-scale in vitro iPSC studies.…”
mentioning
confidence: 99%
“…Furthermore, a lack of standardization in methodology between laboratories likely introduces further variability 9 . While previous work has automated the expansion of individual lines [10][11][12][13] , we reasoned that developing a fully automated, modular platform for parallel iPSC derivation, expansion and differentiation would allow us to identify, and minimize, factors contributing to variability in iPSC behavior as well as provide a platform for large-scale in vitro iPSC studies.…”
mentioning
confidence: 99%
“…Additional limitations of this technique include the difficulty of using this piece of equipment with aseptic techniques; however, we did not encounter high percentages of contamination due to ½ MS media lacking sucrose. One could circumvent any contamination issue by placing the robot in a sterile room, allowing for sterile conditions and cell culture, or by using a liquid handling robot with a sterile chamber 24,25 . Another limitation is tip size and seed aspiration.…”
Section: Discussionmentioning
confidence: 99%
“…This system allows for large sample-sized research, reduced variability, and future high-throughput analysis of the transcriptome and metabolome of progeny cells derived from patient iPSCs. The performance of our system could be deemed to lie between that of simple liquid handlers 11 and a more complex fully integrated platform. 9 Our system enables the maintenance of human fibroblasts undergoing reprogramming; allows for the long-term maintenance, passaging, and differentiation of iPSCs, which are not available with smaller liquid handlers; and offers an economical alternative to more complete equipment.…”
Section: Introductionmentioning
confidence: 99%